Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilK   Type   Machinery gene
Locus tag   GIJ09_RS07005 Genome accession   NZ_CP045832
Coordinates   1284346..1284957 (-) Length   203 a.a.
NCBI ID   WP_010359922.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain AUSMDU00010541     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1232033..1298820 1284346..1284957 within 0


Gene organization within MGE regions


Location: 1232033..1298820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GIJ09_RS06650 (GIJ09_06650) - 1232033..1232686 (-) 654 WP_105211373.1 IS1595 family transposase -
  GIJ09_RS06655 (GIJ09_06655) purM 1233689..1234723 (-) 1035 WP_153707868.1 phosphoribosylformylglycinamidine cyclo-ligase -
  GIJ09_RS06660 (GIJ09_06660) - 1234964..1235152 (+) 189 WP_003689039.1 hypothetical protein -
  GIJ09_RS06665 (GIJ09_06665) - 1235412..1236401 (+) 990 WP_003689040.1 tyrosine-type recombinase/integrase -
  GIJ09_RS06670 (GIJ09_06670) - 1236743..1237222 (+) 480 WP_002241413.1 DUF4760 domain-containing protein -
  GIJ09_RS06675 (GIJ09_06675) - 1237282..1240329 (-) 3048 WP_153707869.1 tape measure protein -
  GIJ09_RS06680 (GIJ09_06680) - 1240359..1240508 (-) 150 WP_003691402.1 hypothetical protein -
  GIJ09_RS06685 (GIJ09_06685) - 1240820..1240993 (-) 174 WP_162855556.1 hypothetical protein -
  GIJ09_RS06690 (GIJ09_06690) - 1240977..1241318 (-) 342 WP_003700102.1 hypothetical protein -
  GIJ09_RS12270 - 1241302..1241460 (-) 159 WP_003691816.1 hypothetical protein -
  GIJ09_RS06695 (GIJ09_06695) - 1241460..1241999 (-) 540 WP_047951643.1 TIGR02594 family protein -
  GIJ09_RS13010 - 1242041..1242166 (-) 126 WP_255294325.1 hypothetical protein -
  GIJ09_RS06700 (GIJ09_06700) - 1242206..1242442 (+) 237 WP_003689049.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  GIJ09_RS06705 (GIJ09_06705) - 1242442..1242789 (+) 348 WP_003689051.1 type II toxin-antitoxin system PemK/MazF family toxin -
  GIJ09_RS06710 (GIJ09_06710) - 1243157..1243489 (-) 333 WP_003691408.1 hypothetical protein -
  GIJ09_RS06715 (GIJ09_06715) - 1243490..1243960 (-) 471 WP_003689057.1 hypothetical protein -
  GIJ09_RS06720 (GIJ09_06720) - 1244031..1244336 (-) 306 WP_003689058.1 hypothetical protein -
  GIJ09_RS06725 (GIJ09_06725) - 1244448..1248593 (-) 4146 WP_153707870.1 phage tail protein -
  GIJ09_RS06730 (GIJ09_06730) - 1248833..1249111 (+) 279 WP_003692877.1 helix-turn-helix domain-containing protein -
  GIJ09_RS06735 (GIJ09_06735) - 1249139..1249570 (-) 432 WP_003689064.1 NlpC/P60 family protein -
  GIJ09_RS06740 (GIJ09_06740) - 1249572..1250426 (-) 855 WP_003698614.1 DUF2163 domain-containing protein -
  GIJ09_RS06745 (GIJ09_06745) - 1250423..1251022 (-) 600 WP_003692874.1 DUF2460 domain-containing protein -
  GIJ09_RS06750 (GIJ09_06750) - 1251022..1251288 (-) 267 WP_003689070.1 hypothetical protein -
  GIJ09_RS06755 (GIJ09_06755) - 1251300..1251629 (-) 330 WP_003689072.1 hypothetical protein -
  GIJ09_RS06760 (GIJ09_06760) - 1251690..1252463 (-) 774 WP_050305212.1 hypothetical protein -
  GIJ09_RS06765 (GIJ09_06765) - 1252489..1252917 (-) 429 WP_003689076.1 hypothetical protein -
  GIJ09_RS06770 (GIJ09_06770) - 1252914..1253396 (-) 483 WP_003703855.1 HK97 gp10 family phage protein -
  GIJ09_RS06775 (GIJ09_06775) - 1253396..1253926 (-) 531 WP_003689080.1 head-tail connector protein -
  GIJ09_RS06780 (GIJ09_06780) - 1253929..1254291 (-) 363 WP_003689082.1 hypothetical protein -
  GIJ09_RS06785 (GIJ09_06785) - 1254298..1255797 (-) 1500 WP_003689084.1 hypothetical protein -
  GIJ09_RS06790 (GIJ09_06790) - 1255838..1256995 (-) 1158 WP_010360058.1 HK97 family phage prohead protease -
  GIJ09_RS06795 (GIJ09_06795) - 1257063..1259210 (-) 2148 WP_064662124.1 phage portal protein -
  GIJ09_RS06800 (GIJ09_06800) terL 1259207..1260628 (-) 1422 WP_003689090.1 phage terminase large subunit -
  GIJ09_RS06805 (GIJ09_06805) - 1260690..1261139 (-) 450 WP_003695485.1 hypothetical protein -
  GIJ09_RS06810 (GIJ09_06810) - 1261432..1262301 (-) 870 WP_149032486.1 Bro-N domain-containing protein -
  GIJ09_RS06815 (GIJ09_06815) - 1262586..1263746 (-) 1161 WP_003691428.1 type I restriction endonuclease -
  GIJ09_RS06820 (GIJ09_06820) - 1263837..1264244 (-) 408 WP_047922557.1 hypothetical protein -
  GIJ09_RS13015 - 1264366..1264494 (+) 129 WP_012503747.1 hypothetical protein -
  GIJ09_RS06830 (GIJ09_06830) - 1264511..1264891 (-) 381 WP_033910829.1 RusA family crossover junction endodeoxyribonuclease -
  GIJ09_RS12690 - 1264882..1265163 (-) 282 WP_003689109.1 hypothetical protein -
  GIJ09_RS06835 (GIJ09_06835) - 1265191..1265340 (-) 150 WP_003689110.1 hypothetical protein -
  GIJ09_RS06840 (GIJ09_06840) - 1265517..1266011 (-) 495 WP_003692852.1 DUF3310 domain-containing protein -
  GIJ09_RS06845 (GIJ09_06845) - 1266103..1266333 (-) 231 WP_033909710.1 hypothetical protein -
  GIJ09_RS06850 (GIJ09_06850) - 1266350..1267711 (-) 1362 WP_003689132.1 DnaB-like helicase C-terminal domain-containing protein -
  GIJ09_RS06855 (GIJ09_06855) - 1267708..1268772 (-) 1065 WP_003689134.1 hypothetical protein -
  GIJ09_RS06860 (GIJ09_06860) - 1268890..1269117 (-) 228 WP_003698261.1 helix-turn-helix domain-containing protein -
  GIJ09_RS06865 (GIJ09_06865) - 1269290..1269478 (+) 189 WP_003696008.1 hypothetical protein -
  GIJ09_RS06870 (GIJ09_06870) - 1269455..1269610 (-) 156 WP_048654358.1 hypothetical protein -
  GIJ09_RS06875 (GIJ09_06875) - 1269690..1269920 (-) 231 WP_020997318.1 transcriptional regulator -
  GIJ09_RS06880 (GIJ09_06880) - 1270049..1270711 (+) 663 WP_012503489.1 LexA family transcriptional regulator -
  GIJ09_RS06885 (GIJ09_06885) - 1270826..1271263 (+) 438 WP_003687967.1 hypothetical protein -
  GIJ09_RS06890 (GIJ09_06890) - 1271280..1271741 (+) 462 WP_003687965.1 helix-turn-helix domain-containing protein -
  GIJ09_RS06895 (GIJ09_06895) - 1271738..1272151 (+) 414 WP_003687963.1 hypothetical protein -
  GIJ09_RS06900 (GIJ09_06900) - 1272349..1272549 (+) 201 WP_050161637.1 hypothetical protein -
  GIJ09_RS06905 (GIJ09_06905) - 1272582..1273058 (+) 477 WP_012504141.1 DUF6948 domain-containing protein -
  GIJ09_RS13020 - 1273055..1273186 (+) 132 WP_267237788.1 hypothetical protein -
  GIJ09_RS06910 (GIJ09_06910) - 1273327..1273659 (+) 333 WP_153707855.1 hypothetical protein -
  GIJ09_RS06915 (GIJ09_06915) - 1273812..1274087 (+) 276 WP_050156283.1 NGO1622 family putative holin -
  GIJ09_RS06920 (GIJ09_06920) - 1274084..1274245 (+) 162 WP_003702497.1 hypothetical protein -
  GIJ09_RS06925 (GIJ09_06925) - 1274314..1275000 (+) 687 WP_012503754.1 phage replication initiation protein, NGO0469 family -
  GIJ09_RS06930 (GIJ09_06930) - 1275140..1275322 (+) 183 WP_003691535.1 hypothetical protein -
  GIJ09_RS06935 (GIJ09_06935) - 1275319..1275810 (+) 492 WP_003691537.1 siphovirus Gp157 family protein -
  GIJ09_RS06940 (GIJ09_06940) - 1275862..1276077 (+) 216 WP_003691538.1 hypothetical protein -
  GIJ09_RS13190 - 1276188..1276454 (+) 267 Protein_1295 hypothetical protein -
  GIJ09_RS06950 (GIJ09_06950) - 1276735..1277418 (+) 684 WP_003687929.1 DUF2786 domain-containing protein -
  GIJ09_RS06955 (GIJ09_06955) - 1277613..1277882 (+) 270 WP_003687928.1 hypothetical protein -
  GIJ09_RS06960 (GIJ09_06960) - 1278238..1279437 (-) 1200 WP_050155689.1 tyrosine-type recombinase/integrase -
  GIJ09_RS06975 (GIJ09_06975) yaaA 1279968..1280747 (-) 780 WP_003706583.1 peroxide stress protein YaaA -
  GIJ09_RS06980 (GIJ09_06980) dapC 1280903..1282090 (-) 1188 WP_149032485.1 succinyldiaminopimelate transaminase -
  GIJ09_RS06985 (GIJ09_06985) dut 1282168..1282620 (-) 453 WP_010359930.1 dUTP diphosphatase -
  GIJ09_RS06990 (GIJ09_06990) - 1282786..1283496 (+) 711 Protein_1302 AzlC family ABC transporter permease -
  GIJ09_RS06995 (GIJ09_06995) - 1283493..1283801 (+) 309 WP_010359926.1 AzlD family protein -
  GIJ09_RS07000 (GIJ09_07000) pilL 1283871..1284344 (-) 474 WP_025455985.1 PilX family type IV pilin Machinery gene
  GIJ09_RS07005 (GIJ09_07005) pilK 1284346..1284957 (-) 612 WP_010359922.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  GIJ09_RS07010 (GIJ09_07010) pilJ 1284936..1285868 (-) 933 WP_153707871.1 PilW family protein Machinery gene
  GIJ09_RS07015 (GIJ09_07015) pilV 1285865..1286476 (-) 612 WP_050164991.1 type IV pilus modification protein PilV Machinery gene
  GIJ09_RS07020 (GIJ09_07020) pilH 1286508..1287173 (-) 666 WP_047918678.1 Tfp pilus assembly protein FimT/FimU Machinery gene
  GIJ09_RS07025 (GIJ09_07025) dnaB 1287481..1288887 (-) 1407 WP_050164993.1 replicative DNA helicase -
  GIJ09_RS07030 (GIJ09_07030) - 1289051..1289632 (+) 582 WP_003690895.1 superoxide dismutase -
  GIJ09_RS07035 (GIJ09_07035) - 1289864..1290373 (-) 510 WP_003687909.1 isoprenylcysteine carboxyl methyltransferase family protein -
  GIJ09_RS07040 (GIJ09_07040) - 1290703..1291035 (-) 333 WP_003687908.1 hypothetical protein -
  GIJ09_RS07045 (GIJ09_07045) cysT 1291221..1292050 (+) 830 Protein_1313 sulfate ABC transporter permease subunit CysT -
  GIJ09_RS07050 (GIJ09_07050) cysW 1292239..1293099 (+) 861 WP_047923066.1 sulfate ABC transporter permease subunit CysW -
  GIJ09_RS07055 (GIJ09_07055) - 1293096..1294172 (+) 1077 WP_003687905.1 sulfate/molybdate ABC transporter ATP-binding protein -
  GIJ09_RS07060 (GIJ09_07060) ilvA 1294228..1295754 (-) 1527 WP_003690890.1 threonine ammonia-lyase, biosynthetic -
  GIJ09_RS07065 (GIJ09_07065) - 1295903..1297072 (+) 1170 WP_003690889.1 D-alanyl-D-alanine carboxypeptidase family protein -
  GIJ09_RS07070 (GIJ09_07070) - 1297198..1297770 (-) 573 WP_003687901.1 50S ribosomal protein L25/general stress protein Ctc -
  GIJ09_RS07075 (GIJ09_07075) - 1297837..1298820 (-) 984 WP_003687900.1 ribose-phosphate pyrophosphokinase -

Sequence


Protein


Download         Length: 203 a.a.        Molecular weight: 22082.01 Da        Isoelectric Point: 8.0862

>NTDB_id=398493 GIJ09_RS07005 WP_010359922.1 1284346..1284957(-) (pilK) [Neisseria gonorrhoeae strain AUSMDU00010541]
MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGEFQVLDLEYAA
DSKVTFSENCEKGLCTAVNVRTNNNGNEEAFGNIVVKGKPTVEAVKRSCPAKSGKNSTDLCIDNQGVEYNKGTAGVSKMP
RYIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=398493 GIJ09_RS07005 WP_010359922.1 1284346..1284957(-) (pilK) [Neisseria gonorrhoeae strain AUSMDU00010541]
ATGCGCAAACAGAACACTTTGACAGGAATCCCGACTTCTGACGGACAGAGGGGGTTCGCACTGTTTATCGTGCTGATGGT
GATGATAGTCGTGGCCTTTTTGGTTGTAACTGCCGCCCAGTCCTACAATACCGAACAGAGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTAGCCGAGGCGGCTTTGCGGGAGGGCGAATTTCAGGTTTTGGATTTGGAATATGCTGCG
GATAGTAAGGTTACATTTAGCGAAAACTGTGAAAAAGGCCTGTGTACCGCAGTGAATGTGCGGACAAATAATAATGGTAA
TGAAGAGGCTTTTGGCAATATCGTGGTGAAAGGCAAGCCCACCGTTGAGGCGGTGAAGCGTTCTTGCCCTGCAAAGTCTG
GCAAAAATTCTACCGACCTGTGCATTGACAATCAGGGAGTGGAATATAACAAAGGCACGGCAGGCGTCAGCAAAATGCCG
CGTTATATTATCGAATATTTAGGCGTGAAGAACGGACAAAATGTTTATCGGGTTACTGCCAAGGCTTGGGGTAAGAATGC
CAATACCGTGGTCGTCCTTCAATCTTATGTAGGCAATAATGATGAGCAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilK Neisseria gonorrhoeae MS11

94.089

100

0.941


Multiple sequence alignment