Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   HR072_RS00885 Genome accession   NZ_CP054230
Coordinates   171648..172316 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain EcPF14     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 166648..177316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR072_RS00855 (HR072_00855) yicC 166667..167530 (-) 864 WP_000621330.1 YicC/YloC family endoribonuclease -
  HR072_RS00860 (HR072_00860) rph 167657..168373 (+) 717 WP_001247093.1 ribonuclease PH -
  HR072_RS00865 (HR072_00865) pyrE 168439..169080 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  HR072_RS00870 (HR072_00870) slmA 169117..169713 (-) 597 WP_033551841.1 nucleoid occlusion factor SlmA -
  HR072_RS00875 (HR072_00875) dut 169820..170278 (-) 459 WP_000976070.1 dUTP diphosphatase -
  HR072_RS00880 (HR072_00880) coaBC 170256..171476 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  HR072_RS00885 (HR072_00885) radC 171648..172316 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  HR072_RS00890 (HR072_00890) rpmB 172533..172769 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  HR072_RS00895 (HR072_00895) rpmG 172790..172957 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  HR072_RS00900 (HR072_00900) mutM 173055..173864 (+) 810 WP_001114542.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  HR072_RS00905 (HR072_00905) coaD 173903..174382 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  HR072_RS00910 (HR072_00910) waaA 174390..175667 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  HR072_RS00915 (HR072_00915) rfaQ 176080..177138 (+) 1059 WP_000360415.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=398378 HR072_RS00885 WP_001297375.1 171648..172316(+) (radC) [Escherichia coli strain EcPF14]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=398378 HR072_RS00885 WP_001297375.1 171648..172316(+) (radC) [Escherichia coli strain EcPF14]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment