Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GII76_RS02340 Genome accession   NZ_CP045826
Coordinates   427505..427954 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain 73     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 422505..432954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII76_RS02305 (GII76_02305) yckA 422658..423338 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  GII76_RS02310 (GII76_02310) yckB 423348..424211 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  GII76_RS02315 (GII76_02315) - 424373..424570 (+) 198 WP_119122841.1 hypothetical protein -
  GII76_RS02320 (GII76_02320) yckC 424589..425044 (+) 456 WP_029727075.1 RDD family protein -
  GII76_RS02325 (GII76_02325) yckD 425126..425458 (+) 333 WP_015252849.1 YckD family protein -
  GII76_RS02330 (GII76_02330) bglC 425610..427043 (+) 1434 WP_029727076.1 6-phospho-beta-glucosidase -
  GII76_RS02335 (GII76_02335) nin/comJ 427080..427478 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  GII76_RS02340 (GII76_02340) nucA/comI 427505..427954 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  GII76_RS02345 (GII76_02345) tlpC 428122..429843 (-) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  GII76_RS02350 (GII76_02350) hxlB 429954..430511 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  GII76_RS02355 (GII76_02355) hxlA 430517..431149 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  GII76_RS02360 (GII76_02360) hxlR 431382..431744 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=398352 GII76_RS02340 WP_003234608.1 427505..427954(-) (nucA/comI) [Bacillus subtilis strain 73]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=398352 GII76_RS02340 WP_003234608.1 427505..427954(-) (nucA/comI) [Bacillus subtilis strain 73]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment