Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GII78_RS01975 Genome accession   NZ_CP045824
Coordinates   370422..370871 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain MB8_B10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365422..375871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII78_RS01940 (GII78_01940) yckA 365575..366255 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  GII78_RS01945 (GII78_01945) yckB 366265..367128 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  GII78_RS01950 (GII78_01950) - 367290..367487 (+) 198 WP_119122841.1 hypothetical protein -
  GII78_RS01955 (GII78_01955) yckC 367506..367961 (+) 456 WP_029727075.1 RDD family protein -
  GII78_RS01960 (GII78_01960) yckD 368043..368375 (+) 333 WP_015252849.1 YckD family protein -
  GII78_RS01965 (GII78_01965) bglC 368527..369960 (+) 1434 WP_029727076.1 6-phospho-beta-glucosidase -
  GII78_RS01970 (GII78_01970) nin/comJ 369997..370395 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  GII78_RS01975 (GII78_01975) nucA/comI 370422..370871 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  GII78_RS01980 (GII78_01980) tlpC 371039..372760 (-) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  GII78_RS01985 (GII78_01985) hxlB 372871..373428 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  GII78_RS01990 (GII78_01990) hxlA 373434..374066 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  GII78_RS01995 (GII78_01995) hxlR 374299..374661 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=398192 GII78_RS01975 WP_003234608.1 370422..370871(-) (nucA/comI) [Bacillus subtilis strain MB8_B10]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=398192 GII78_RS01975 WP_003234608.1 370422..370871(-) (nucA/comI) [Bacillus subtilis strain MB8_B10]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment