Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GII81_RS14000 Genome accession   NZ_CP045820
Coordinates   2680282..2680692 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain MB9_B1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2675282..2685692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII81_RS13970 (GII81_13970) - 2675404..2675544 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  GII81_RS13975 (GII81_13975) yqeF 2675850..2676581 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  GII81_RS13980 (GII81_13980) cwlH 2676833..2677585 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  GII81_RS13985 (GII81_13985) yqeD 2677772..2678398 (+) 627 WP_029726699.1 TVP38/TMEM64 family protein -
  GII81_RS13990 (GII81_13990) gnd 2678417..2679285 (-) 869 Protein_2731 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  GII81_RS13995 (GII81_13995) yqeB 2679527..2680249 (+) 723 WP_010886572.1 hypothetical protein -
  GII81_RS14000 (GII81_14000) nucA/comI 2680282..2680692 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  GII81_RS14005 (GII81_14005) - 2680888..2681235 (+) 348 Protein_2734 sigma-70 family RNA polymerase sigma factor -
  GII81_RS14010 (GII81_14010) spoIVCA 2681266..2682726 (-) 1461 WP_250620716.1 site-specific DNA recombinase SpoIVCA -
  GII81_RS22460 - 2682684..2682862 (-) 179 Protein_2736 hypothetical protein -
  GII81_RS14015 (GII81_14015) arsC 2683217..2683636 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  GII81_RS14020 (GII81_14020) acr3 2683648..2684688 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  GII81_RS14025 (GII81_14025) arsK 2684711..2685151 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  GII81_RS14030 (GII81_14030) arsR 2685212..2685529 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=397993 GII81_RS14000 WP_009967785.1 2680282..2680692(-) (nucA/comI) [Bacillus subtilis strain MB9_B1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=397993 GII81_RS14000 WP_009967785.1 2680282..2680692(-) (nucA/comI) [Bacillus subtilis strain MB9_B1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment