Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GII83_RS12855 Genome accession   NZ_CP045818
Coordinates   2504665..2505075 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain MB9_B6     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2499665..2510075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII83_RS12825 (GII83_12825) - 2499787..2499927 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  GII83_RS12830 (GII83_12830) yqeF 2500233..2500964 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  GII83_RS12835 (GII83_12835) cwlH 2501216..2501968 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  GII83_RS12840 (GII83_12840) yqeD 2502155..2502781 (+) 627 WP_029726699.1 TVP38/TMEM64 family protein -
  GII83_RS12845 (GII83_12845) gnd 2502800..2503668 (-) 869 Protein_2497 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  GII83_RS12850 (GII83_12850) yqeB 2503910..2504632 (+) 723 WP_010886572.1 hypothetical protein -
  GII83_RS12855 (GII83_12855) nucA/comI 2504665..2505075 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  GII83_RS12860 (GII83_12860) - 2505271..2505618 (+) 348 Protein_2500 sigma-70 family RNA polymerase sigma factor -
  GII83_RS12865 (GII83_12865) spoIVCA 2505649..2507109 (-) 1461 WP_250620044.1 site-specific DNA recombinase SpoIVCA -
  GII83_RS21270 - 2507067..2507245 (-) 179 Protein_2502 hypothetical protein -
  GII83_RS12870 (GII83_12870) - 2508158..2508370 (+) 213 WP_029726695.1 spore germination protein -
  GII83_RS12875 (GII83_12875) - 2508738..2508831 (+) 94 Protein_2504 spore germination protein -
  GII83_RS12880 (GII83_12880) - 2509115..2509825 (-) 711 WP_029726694.1 DUF421 domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=397833 GII83_RS12855 WP_009967785.1 2504665..2505075(-) (nucA/comI) [Bacillus subtilis strain MB9_B6]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=397833 GII83_RS12855 WP_009967785.1 2504665..2505075(-) (nucA/comI) [Bacillus subtilis strain MB9_B6]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment