Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BSN5_RS12165 Genome accession   NC_014976
Coordinates   2320994..2322370 (+) Length   458 a.a.
NCBI ID   WP_015715115.1    Uniprot ID   A0A8E0SBR9
Organism   Bacillus subtilis BSn5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2315994..2327370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSN5_RS12145 (BSn5_11985) ctsR 2316347..2316811 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  BSN5_RS12150 (BSn5_11990) mcsA 2316825..2317382 (+) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  BSN5_RS12155 (BSn5_11995) mcsB 2317382..2318473 (+) 1092 WP_003235007.1 protein arginine kinase -
  BSN5_RS12160 (BSn5_12000) clpC 2318470..2320902 (+) 2433 WP_015715114.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BSN5_RS12165 (BSn5_12005) radA 2320994..2322370 (+) 1377 WP_015715115.1 DNA repair protein RadA Machinery gene
  BSN5_RS12170 (BSn5_12010) disA 2322374..2323456 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  BSN5_RS12175 (BSn5_12015) yacL 2323572..2324672 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  BSN5_RS12180 (BSn5_12020) ispD 2324687..2325385 (+) 699 WP_015252991.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BSN5_RS12185 (BSn5_12025) ispF 2325378..2325854 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49454.94 Da        Isoelectric Point: 8.2399

>NTDB_id=39780 BSN5_RS12165 WP_015715115.1 2320994..2322370(+) (radA) [Bacillus subtilis BSn5]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPSFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=39780 BSN5_RS12165 WP_015715115.1 2320994..2322370(+) (radA) [Bacillus subtilis BSn5]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCATCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCTGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCACTCATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTAGG
GCTGCTGCTGCAAAATCAGGATGCATATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGCTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTAAAAGAAGCGGCAAAGCTTGGTTTTAAGCGCATGATCATACC
CGCGGCAAATCTGGACGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGAA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.782

100

0.998
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624

Multiple sequence alignment