Detailed information NTG0397315

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: N028_RS21770
• Genome accession: NZ_CP045799
• Coordinates: 4967524..4968891 (-)
• Length: 455 a.a.
• NCBI ID: WP_024640108.1
• Uniprot ID: -
• Organism: Pseudomonas syringae USA011
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	4969820..4971195	4967524..4968891	flank	929

Gene organization within MGE regions

Location: 4967524..4971195

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N028_RS21770 (N028_21770)	radA	4967524..4968891 (-)	1368	WP_024640108.1	DNA repair protein RadA	Machinery gene
N028_RS21775 (N028_21775)	mscL	4969088..4969534 (+)	447	WP_003399960.1	large-conductance mechanosensitive channel protein MscL	-
N028_RS21780 (N028_21780)	-	4969820..4971195 (+)	1376	WP_104722995.1	IS3-like element ISPsy9 family transposase	-

Sequence

Protein

• Length: 455 a.a.
• Molecular weight: 48941.53 Da
• Isoelectric Point: 7.1316

>NTDB_id=397315 N028_RS21770 WP_024640108.1 4967524..4968891(-) (radA) [Pseudomonas syringae USA011]
MVKAKRLYGCTECGATFPKWAGQCSECGAWNTLVETMIESGAAAPPTGRTGWTGSQAQIRTLAEVSVEEIPRFSTNSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIA
TAKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide

• Length: 1368 bp

>NTDB_id=397315 N028_RS21770 WP_024640108.1 4967524..4968891(-) (radA) [Pseudomonas syringae USA011]
ATGGTCAAGGCCAAACGCTTGTACGGCTGCACCGAGTGCGGCGCGACATTTCCCAAGTGGGCGGGCCAGTGCAGCGAATG
CGGTGCCTGGAACACACTGGTCGAAACCATGATTGAAAGCGGTGCCGCCGCGCCGCCTACCGGGCGTACCGGCTGGACCG
GTTCCCAGGCGCAGATCCGGACCCTGGCGGAAGTCAGCGTCGAAGAGATTCCGCGCTTCTCGACCAACTCCACCGAGCTG
GACAGGGTGCTCGGCGGCGGTCTGGTTGACGGTTCGGTGGTGCTGATTGGCGGCGACCCTGGCATCGGCAAGTCCACGAT
CCTCTTGCAGACGCTGTGCAACATCGCCCAGCGCATGCCCGCGCTGTATGTCACCGGTGAGGAATCCCAGCAGCAGGTCG
CCATGCGCGCCCGGCGCTTGGGGCTGCCGCAGGACAAGCTGCGGGTCATGACCGAAACCTGCATCGAGACCATCATTGCC
ACCGCCAAGGTCGAGAAGCCCAAGGTGATGGTGATCGATTCGATCCAGACGATTTTCACCGAACAGCTGCAGTCGGCCCC
TGGCGGCGTGTCGCAGGTTCGCGAAAGCGCCGCCTTGCTGGTGCGCTACGCCAAGCAGAGCGGTACGGCGATCTTCCTGG
TCGGCCACGTCACCAAGGAAGGTGCACTGGCCGGCCCGCGTGTGCTTGAGCACATGGTCGACACCGTGTTGTATTTCGAG
GGCGAATCGGACGGCCGCCTGCGTTTGCTGCGCGCGGTGAAGAACCGCTTTGGCGCGGTCAACGAGCTGGGCGTGTTCGG
CATGACCGACAAGGGCCTGAAAGAAGTCTCCAACCCGTCGGCGATTTTTCTCACCCGTGCGCAGGAAGAAGTGCCGGGCA
GTGTGGTCATGGCCACCTGGGAGGGCACGCGGCCGATGCTGGTCGAGGTGCAGGCGCTGGTGGACGACAGTCACATGTCC
AATCCGCGCCGCGTAACGCTGGGTCTGGATCAGAATCGTCTGGCCATGTTGCTGGCGGTCCTGCACCGACATGGCGGCAT
TCCCACTCACGATCAGGACGTGTTTCTCAACGTGGTGGGTGGTGTGAAGGTGCTGGAAACCGCCTCCGACCTGGCCTTGA
TGGCAGCGGTGATGTCCAGCCTGCGCAATCGTCCGTTGCCGCATGACTTGCTGGTGTTCGGTGAGGTTGGGCTGTCGGGT
GAAGTCAGGCCGGTGCCCAGCGGCCAGGAGCGCCTCAAGGAGGCCGCCAAGCACGGCTTCAAGCGCGCCATCGTGCCCAA
GGGCAATGCGCCGAAGGAAGCGCCGCCCGGCTTGCAGATCATTGCCGTTACGCGCCTGGAGCAGGCCCTGGATGCGCTGT
TCGAGTAA

Domains

Predicted by InterproScan.

• AAA domain (AAA_25) (73-221)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

• Subunit ChlI of Mg-chelatase (ChlI) (353-429)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	48.904	100	0.49
radA	Streptococcus pneumoniae Rx1	46.522	100	0.47
radA	Streptococcus pneumoniae R6	46.522	100	0.47
radA	Streptococcus pneumoniae TIGR4	46.522	100	0.47
radA	Streptococcus pneumoniae D39	46.522	100	0.47
radA	Streptococcus mitis SK321	46.711	100	0.468
radA	Streptococcus mitis NCTC 12261	46.491	100	0.466