Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   TSC_RS02770 Genome accession   NC_014974
Coordinates   516035..516949 (-) Length   304 a.a.
NCBI ID   WP_015716476.1    Uniprot ID   E8PM39
Organism   Thermus scotoductus SA-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 511035..521949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TSC_RS02755 (TSC_c05600) - 511305..511724 (-) 420 WP_015716474.1 disulfide bond formation protein B -
  TSC_RS02760 (TSC_c05610) - 511837..513645 (-) 1809 WP_041438060.1 ATP-binding protein -
  TSC_RS02765 - 513650..514801 (-) 1152 WP_041438063.1 DNA double-strand break repair nuclease NurA -
  TSC_RS12570 - 514822..516000 (-) 1179 WP_200855933.1 Druantia anti-phage system protein DruA -
  TSC_RS02770 (TSC_c05620) pilA 516035..516949 (-) 915 WP_015716476.1 signal recognition particle-docking protein FtsY Machinery gene
  TSC_RS02775 (TSC_c05630) - 516993..517433 (-) 441 WP_015716477.1 hypothetical protein -
  TSC_RS02780 (TSC_c05640) gltX 517465..518883 (-) 1419 WP_015716478.1 glutamate--tRNA ligase -
  TSC_RS02785 (TSC_c05650) lptB 518938..519666 (+) 729 WP_015716479.1 LPS export ABC transporter ATP-binding protein -
  TSC_RS02790 (TSC_c05660) - 519670..521055 (+) 1386 WP_015716480.1 DUF3084 domain-containing protein -
  TSC_RS12575 prmC 520963..521820 (-) 858 WP_081454904.1 peptide chain release factor N(5)-glutamine methyltransferase -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 32924.18 Da        Isoelectric Point: 7.3622

>NTDB_id=39712 TSC_RS02770 WP_015716476.1 516035..516949(-) (pilA) [Thermus scotoductus SA-01]
MGFFDRLKAGLAKTRETLLKAIPWGGSPEEVLEELEMALLAADVGLEATEELLAEVRASGRKDLKEAVKEKLVQMLEPDE
RRATLRKLGFRPQNPKPIEPQGHVVLVVGVNGVGKTTTIAKLGRYYRNLGKKVMFCAGDTFRAAGGAQLSEWGKRLDIPV
IQGPEGADPAALAFDAASARKARGYDLLLVDTAGRLHTKHNLMEELKKVKRAIAKADPEEPKEVWLVLDAVTGQNGLEQA
KRFHEAVGLTGVIVTKLDGTAKGGVLIPIVRTLKVPIRFIGVGEGPDDLQPFDAEAFVEALLEG

Nucleotide


Download         Length: 915 bp        

>NTDB_id=39712 TSC_RS02770 WP_015716476.1 516035..516949(-) (pilA) [Thermus scotoductus SA-01]
ATGGGCTTCTTTGACCGATTGAAAGCCGGCCTCGCCAAGACGCGGGAAACCCTCCTCAAGGCCATCCCCTGGGGCGGAAG
CCCGGAGGAGGTCCTGGAGGAGCTGGAAATGGCCCTCCTTGCCGCCGACGTGGGCCTCGAGGCCACCGAGGAACTCCTGG
CCGAGGTAAGGGCCTCGGGGCGGAAGGACCTGAAGGAGGCCGTTAAGGAGAAGCTCGTCCAGATGCTGGAGCCGGACGAG
CGCCGGGCCACCTTGAGGAAGCTCGGCTTCCGCCCGCAAAACCCCAAGCCCATAGAACCCCAGGGCCACGTGGTCCTGGT
GGTGGGGGTGAACGGGGTGGGCAAGACCACCACCATCGCCAAGCTGGGCCGCTACTACCGGAACCTGGGCAAGAAGGTGA
TGTTCTGCGCCGGGGACACCTTCCGCGCCGCCGGGGGAGCCCAGCTTTCCGAGTGGGGGAAACGCCTGGACATCCCCGTG
ATCCAGGGGCCGGAAGGAGCCGACCCCGCCGCTTTGGCCTTTGATGCGGCAAGCGCCCGGAAAGCCAGGGGCTACGACCT
CCTCCTGGTGGACACCGCAGGCCGCCTCCACACCAAGCACAACCTGATGGAGGAGCTGAAGAAGGTGAAGCGGGCCATCG
CCAAGGCCGATCCCGAGGAGCCCAAGGAGGTCTGGCTGGTGCTGGATGCCGTCACCGGGCAAAACGGCCTGGAGCAGGCC
AAGCGCTTCCACGAGGCGGTGGGGCTCACCGGGGTCATCGTCACCAAGCTGGACGGCACCGCCAAGGGCGGGGTCCTCAT
CCCCATCGTGCGCACCCTTAAGGTGCCCATCCGGTTCATCGGGGTAGGGGAAGGCCCCGACGACCTCCAACCCTTCGACG
CCGAGGCCTTTGTGGAGGCGTTATTGGAGGGGTAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(29-70)

SRP54-type protein, GTPase domain (SRP54)

(104-302)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E8PM39

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.363

100

0.51

Multiple sequence alignment