Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GFD20_RS19675 Genome accession   NZ_CP045768
Coordinates   4296482..4297843 (-) Length   453 a.a.
NCBI ID   WP_003094867.1    Uniprot ID   A0A0H2ZHH7
Organism   Pseudomonas aeruginosa strain CFSAN084950     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4291482..4302843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFD20_RS19650 (GFD20_19650) yjiA 4291669..4292673 (-) 1005 WP_003094852.1 GTPase -
  GFD20_RS19655 (GFD20_19655) - 4292733..4292936 (-) 204 WP_003094856.1 YbdD/YjiX family protein -
  GFD20_RS19660 (GFD20_19660) - 4292985..4295051 (-) 2067 WP_003094859.1 carbon starvation CstA family protein -
  GFD20_RS19665 (GFD20_19665) - 4295389..4295895 (-) 507 WP_003094862.1 DUF3015 domain-containing protein -
  GFD20_RS19670 (GFD20_19670) mapZ 4296098..4296475 (+) 378 WP_003094864.1 cyclic di-GMP-binding protein MapZ -
  GFD20_RS19675 (GFD20_19675) radA 4296482..4297843 (-) 1362 WP_003094867.1 DNA repair protein RadA Machinery gene
  GFD20_RS19680 (GFD20_19680) - 4297953..4298387 (+) 435 WP_003104889.1 CopD family protein -
  GFD20_RS19685 (GFD20_19685) - 4298449..4298703 (-) 255 WP_003104887.1 YdcH family protein -
  GFD20_RS19690 (GFD20_19690) - 4298778..4299329 (-) 552 WP_025271364.1 ankyrin repeat domain-containing protein -
  GFD20_RS19695 (GFD20_19695) katB 4299383..4300924 (-) 1542 WP_128570765.1 catalase KatB -
  GFD20_RS29725 - 4300921..4301409 (-) 489 WP_169433242.1 hypothetical protein -
  GFD20_RS19705 (GFD20_19705) mscL 4301452..4301865 (+) 414 WP_003104883.1 large-conductance mechanosensitive channel protein MscL -
  GFD20_RS19710 (GFD20_19710) - 4301971..4302747 (-) 777 WP_023084835.1 ferredoxin--NADP reductase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48410.81 Da        Isoelectric Point: 6.8357

>NTDB_id=396981 GFD20_RS19675 WP_003094867.1 4296482..4297843(-) (radA) [Pseudomonas aeruginosa strain CFSAN084950]
MAKAKRMYGCTECGATFPKWAGQCADCGAWNTLVETVVEAAPSGSGRGGWAGQQANLKTLAEVSVEEMPRFTTGSTELDR
VLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLASRVPALYVTGEESQQQVAMRARRLSLPEDKLKVMTETSIETIIATA
RQEQPRVMVIDSIQTIFTEQLQSAPGGVAQVRESAAMLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFEGE
SDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEAVPGSVVMATWEGSRPMLVEVQALVDTSHLANP
RRVTLGLDQNRLAMLLAVLHRHGGIPTYDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSGEV
RPVPSGQERLKEAGKHGFKRAIVPLGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=396981 GFD20_RS19675 WP_003094867.1 4296482..4297843(-) (radA) [Pseudomonas aeruginosa strain CFSAN084950]
ATGGCCAAGGCCAAGCGCATGTACGGCTGCACCGAGTGTGGCGCGACCTTCCCGAAATGGGCCGGCCAGTGCGCGGACTG
CGGGGCCTGGAACACCCTGGTGGAGACCGTCGTCGAGGCCGCGCCGAGCGGCTCCGGCCGCGGCGGCTGGGCCGGCCAGC
AGGCCAACCTGAAGACCCTGGCGGAGGTCAGCGTCGAGGAAATGCCGCGCTTCACGACCGGCTCCACCGAACTGGACCGG
GTTCTCGGCGGCGGCCTGGTGGACGGTTCGGTGGTGCTGATCGGCGGCGATCCCGGCATCGGCAAGTCGACCATTCTCCT
GCAGACCCTTTGCAACCTGGCCAGCCGCGTCCCGGCGCTGTACGTCACCGGCGAGGAATCCCAGCAGCAGGTGGCGATGC
GCGCGCGGCGCCTGTCGTTGCCGGAAGACAAGCTCAAGGTGATGACCGAGACCAGCATCGAAACCATCATCGCCACCGCG
CGCCAGGAGCAGCCGCGGGTGATGGTGATCGACTCGATCCAGACCATCTTCACCGAGCAGTTGCAATCGGCGCCGGGCGG
CGTCGCCCAGGTCCGCGAGAGCGCCGCGATGCTGGTGCGCTATGCCAAGCAGAGCGGCACGGCGATCTTCCTGGTCGGCC
ACGTGACCAAGGAAGGCGCCCTGGCCGGCCCGCGGGTGCTCGAACACATGGTCGACACCGTACTGTATTTCGAGGGCGAA
TCAGACGGCCGCCTGCGCCTGCTGCGGGCGGTGAAGAACCGCTTCGGCGCGGTCAACGAACTGGGAGTGTTCGGCATGAC
CGACAAAGGCCTGAAGGAAGTGAGCAACCCTTCGGCGATCTTCCTCACGCGGGCCCAGGAAGCGGTGCCCGGCAGCGTGG
TGATGGCCACCTGGGAAGGCTCGCGGCCGATGCTGGTGGAGGTCCAGGCGCTGGTCGACACCAGTCACCTGGCGAATCCG
CGGCGGGTGACCCTGGGCCTCGACCAGAACCGCCTGGCGATGCTGCTGGCGGTGCTGCACCGGCACGGCGGTATCCCGAC
CTACGACCAGGACGTGTTCCTCAACGTGGTGGGTGGGGTCAAGGTGCTGGAAACCGCCTCCGACCTGGCGTTGATGGCGG
CGGTGATGTCCAGCCTGCGCAACCGCCCGCTGCCGCACGACCTGCTGGTGTTCGGCGAGGTCGGGCTGTCCGGCGAGGTG
CGTCCGGTGCCGAGCGGCCAGGAGCGCCTGAAGGAAGCCGGCAAGCATGGTTTCAAGCGTGCCATCGTACCCCTCGGCAA
CGCGCCGAAGGAGGCGCCGGCGGGATTGCAGGTGATCGCCGTGACGCGCCTCGAGCAGGCCCTGGACGCCCTCTTCGAGT
GA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(74-219)

Subunit ChlI of Mg-chelatase (ChlI)

(361-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZHH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.458

100

0.486
  radA Streptococcus pneumoniae Rx1

46.288

100

0.468
  radA Streptococcus pneumoniae D39

46.288

100

0.468
  radA Streptococcus pneumoniae R6

46.288

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.288

100

0.468
  radA Streptococcus mitis SK321

46.389

100

0.468
  radA Streptococcus mitis NCTC 12261

46.171

100

0.466

Multiple sequence alignment