Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EE896_RS16390 Genome accession   NZ_CP045720
Coordinates   3508634..3510016 (-) Length   460 a.a.
NCBI ID   WP_003854897.1    Uniprot ID   A0A1I5CYX4
Organism   Pantoea eucalypti strain LMG 24197     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3503634..3515016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EE896_RS16375 (EE896_16375) - 3505187..3506083 (-) 897 WP_003854894.1 LysR family transcriptional regulator -
  EE896_RS16380 (EE896_16380) - 3506270..3507271 (+) 1002 WP_003854895.1 zinc-binding alcohol dehydrogenase family protein -
  EE896_RS16385 (EE896_16385) nadR 3507317..3508558 (-) 1242 WP_008925532.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  EE896_RS16390 (EE896_16390) radA 3508634..3510016 (-) 1383 WP_003854897.1 DNA repair protein RadA Machinery gene
  EE896_RS16395 (EE896_16395) serB 3510030..3511007 (-) 978 WP_078804764.1 phosphoserine phosphatase -
  EE896_RS16400 (EE896_16400) - 3511115..3511765 (+) 651 WP_008925530.1 YtjB family periplasmic protein -
  EE896_RS16405 (EE896_16405) yjjG 3512087..3512767 (-) 681 WP_140033776.1 pyrimidine 5'-nucleotidase -
  EE896_RS16410 (EE896_16410) rimI 3512786..3513226 (-) 441 WP_003850861.1 ribosomal protein S18-alanine N-acetyltransferase -
  EE896_RS16415 (EE896_16415) - 3513198..3513608 (-) 411 WP_003850863.1 DNA polymerase III subunit psi -
  EE896_RS16420 (EE896_16420) rsmC 3513715..3514746 (+) 1032 WP_008925528.1 16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49323.93 Da        Isoelectric Point: 7.4220

>NTDB_id=396353 EE896_RS16390 WP_003854897.1 3508634..3510016(-) (radA) [Pantoea eucalypti strain LMG 24197]
MAKAAKRAFVCNECGADYPRWQGQCSACHAWNTITEVRIAASPAAARNERLSGYAGSAGTSRVQKLSEISLEALPRFSTS
FKEFDRVLGGGVVPGSAILIGGSPGAGKSTLLLQVMCRLSEGMKTLYVTGEESLQQVAMRAHRLGLPTENVNMLSETSIE
QICLIAEQEQPQLMVIDSIQVMHMAEIQSSPGSVAQVRETAAYLTRFAKTRGVAIIMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGMREVSNPSAIFLSRGEEVTSGSSVMVLWEGTRPLLVEIQALVDH
SMMGNPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALMLSMVSSLRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERISEAAKHGFKRAIVPAGNAPKKPIAGMQVFSAKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=396353 EE896_RS16390 WP_003854897.1 3508634..3510016(-) (radA) [Pantoea eucalypti strain LMG 24197]
TTGGCCAAAGCGGCAAAACGCGCATTTGTCTGTAACGAATGTGGCGCAGATTACCCACGCTGGCAGGGGCAATGCAGCGC
CTGTCACGCCTGGAACACCATCACCGAGGTGCGCATCGCTGCCTCGCCTGCTGCTGCGCGTAACGAGCGCCTGAGTGGCT
ATGCCGGCAGCGCCGGAACCAGCCGGGTGCAGAAGCTCTCTGAAATCAGCCTGGAGGCGTTACCGCGCTTCTCGACCAGC
TTCAAAGAGTTTGACCGGGTGCTGGGCGGCGGCGTTGTGCCGGGCAGCGCCATTCTGATTGGCGGCAGTCCTGGTGCGGG
GAAATCCACGCTGCTGCTGCAGGTGATGTGCCGGTTGTCAGAGGGGATGAAAACCCTGTACGTCACCGGTGAAGAGTCAC
TGCAACAGGTTGCGATGCGCGCCCATCGTCTGGGATTACCCACCGAAAATGTGAATATGCTGTCGGAAACCAGCATTGAG
CAGATCTGCCTGATCGCCGAACAGGAGCAGCCGCAGCTGATGGTGATCGACTCGATCCAGGTCATGCACATGGCAGAGAT
CCAGTCATCACCCGGCAGCGTCGCGCAGGTGCGTGAAACCGCCGCCTACCTGACGCGCTTCGCTAAAACGCGCGGCGTCG
CGATCATCATGGTCGGTCACGTGACTAAAGATGGCTCACTGGCCGGGCCGAAAGTGCTGGAGCACTGTATCGACTGTTCG
GTGCTGCTGGATGGCGATGCCGACTCCCGTTTCCGCACCCTTCGCAGCCATAAAAACCGCTTCGGTGCGGTCAATGAGCT
GGGGGTGTTTGCCATGACAGAGCAGGGCATGCGTGAAGTCAGCAACCCGTCCGCTATCTTCCTGTCACGGGGCGAAGAGG
TTACCTCTGGCAGCTCGGTGATGGTGCTGTGGGAAGGCACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCCATGATGGGCAACCCACGCCGCGTGGCGGTGGGTCTGGAGCAGAACCGCCTGGCGATACTGCTGGCCGTTTTACATCG
TCATGGCGGATTGCAGATGGCGGATCAGGATGTATTCGTCAACGTGGTTGGTGGTGTTAAAGTCACCGAAACCAGTGCGG
ACCTGGCGCTGATGCTGTCGATGGTGTCGAGCCTGCGCGATCGTCCGTTGCCGCAGGATCTGGTGATCTTCGGCGAAGTG
GGACTGGCCGGTGAGATCCGTCCGGTGCCCAGCGGTCAGGAGCGCATCTCTGAAGCGGCGAAGCATGGCTTCAAACGCGC
CATTGTACCGGCAGGGAATGCACCGAAAAAACCGATTGCCGGGATGCAGGTTTTCAGCGCGAAGAAGCTGGCCGATGCGC
TGGCGATTCTGGACGATCTGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(347-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(87-225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I5CYX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.373

100

0.485
  radA Streptococcus mitis NCTC 12261

44.82

96.522

0.433
  radA Streptococcus pneumoniae Rx1

45.244

93.696

0.424
  radA Streptococcus pneumoniae D39

45.244

93.696

0.424
  radA Streptococcus pneumoniae R6

45.244

93.696

0.424
  radA Streptococcus pneumoniae TIGR4

45.244

93.696

0.424
  radA Streptococcus mitis SK321

45.244

93.696

0.424

Multiple sequence alignment