Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GFU70_RS24675 Genome accession   NZ_CP045701
Coordinates   5689680..5691047 (-) Length   455 a.a.
NCBI ID   WP_030140305.1    Uniprot ID   A0A0B1YXD7
Organism   Pseudomonas brassicacearum strain S-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5684680..5696047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFU70_RS24650 (GFU70_24645) - 5684886..5685605 (+) 720 WP_116641300.1 GntR family transcriptional regulator -
  GFU70_RS24655 (GFU70_24650) yjiA 5685597..5686565 (-) 969 WP_058544062.1 GTPase -
  GFU70_RS24660 (GFU70_24655) - 5686744..5686941 (-) 198 WP_153388929.1 YbdD/YjiX family protein -
  GFU70_RS24665 (GFU70_24660) - 5686957..5689023 (-) 2067 WP_153388930.1 carbon starvation CstA family protein -
  GFU70_RS24670 (GFU70_24665) - 5689182..5689538 (+) 357 WP_058544500.1 PilZ domain-containing protein -
  GFU70_RS24675 (GFU70_24670) radA 5689680..5691047 (-) 1368 WP_030140305.1 DNA repair protein RadA Machinery gene
  GFU70_RS24680 (GFU70_24675) - 5691091..5691639 (-) 549 WP_058542553.1 ankyrin repeat domain-containing protein -
  GFU70_RS24685 (GFU70_24680) katB 5691792..5693333 (-) 1542 WP_153388931.1 catalase KatB -
  GFU70_RS24690 (GFU70_24685) mscL 5693616..5694029 (+) 414 WP_058542551.1 large-conductance mechanosensitive channel protein MscL -
  GFU70_RS24695 (GFU70_24690) - 5694260..5695036 (-) 777 WP_153388932.1 ferredoxin--NADP reductase -
  GFU70_RS24700 (GFU70_24695) - 5695327..5696040 (+) 714 WP_058542549.1 autoinducer binding domain-containing protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48774.24 Da        Isoelectric Point: 6.8987

>NTDB_id=396113 GFU70_RS24675 WP_030140305.1 5689680..5691047(-) (radA) [Pseudomonas brassicacearum strain S-1]
MAKAKRMYGCTECGSTFPKWAGQCSECGAWNTLTETMVESGGAAAPTGRTGWAGQQAQIKTLAEVSVEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLATRMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARLEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=396113 GFU70_RS24675 WP_030140305.1 5689680..5691047(-) (radA) [Pseudomonas brassicacearum strain S-1]
ATGGCCAAGGCAAAGCGCATGTACGGCTGCACCGAGTGCGGCTCGACCTTTCCCAAATGGGCCGGCCAGTGCAGCGAATG
CGGGGCCTGGAACACCCTGACTGAAACCATGGTGGAAAGCGGCGGTGCGGCGGCACCCACCGGTCGCACGGGCTGGGCTG
GTCAGCAGGCGCAGATCAAGACCCTGGCCGAAGTCAGCGTTGAAGAAATTCCGCGTTTCTCCACCGCCTCCGGTGAGCTG
GACCGCGTGCTGGGCGGCGGCCTGGTGGACGGTTCGGTGGTGCTGATCGGCGGCGATCCGGGCATCGGCAAATCCACCAT
CTTGTTGCAAACCTTGTGCAACCTCGCCACCCGCATGCCGGCACTGTATGTCACTGGCGAAGAGTCCCAGCAGCAAGTCG
CCATGCGCGCCCGACGCCTGGGCTTGCCCCAGGACCAGTTGCGGGTCATGACCGAAACCTGCATCGAGACCATCATCGCC
ACGGCGCGCCTGGAAAAACCCAAGGTGATGGTGATCGACTCGATCCAGACCATTTTCACCGAACAGCTGCAATCGGCACC
GGGCGGCGTGTCCCAGGTGCGCGAAAGCGCGGCGTTGCTGGTGCGCTACGCCAAGCAGAGTGGCACGGCGATTTTCCTGG
TGGGCCACGTCACCAAGGAGGGCGCACTGGCGGGGCCGCGGGTGCTGGAACACATGGTCGATACGGTGTTGTATTTCGAG
GGCGAATCCGACGGACGCCTGCGTTTGCTGCGGGCGGTGAAGAACCGCTTTGGCGCCGTCAACGAGCTGGGTGTGTTCGG
CATGACCGACAGAGGCTTGAAAGAAGTCTCAAACCCTTCGGCGATCTTTCTGACGCGTGCCCAGGAAGAAGTCCCCGGCA
GCGTGGTCATGGCAACATGGGAAGGCACCCGGCCGATGTTGGTCGAAGTGCAAGCGCTGGTGGACGACAGCCATCTGGCG
AACCCGCGCCGGGTCACCCTGGGCCTGGATCAGAATCGGTTGGCCATGTTGCTGGCGGTGTTGCATCGACACGGCGGGAT
TCCGACGCACGACCAGGATGTTTTCCTCAACGTCGTGGGCGGGGTCAAGGTGCTGGAAACCGCATCCGACCTGGCGTTGA
TGGCCGCGGTCATGTCCAGCCTGCGCAACCGGCCGTTGCCCCACGATCTGTTGGTGTTCGGCGAAGTGGGGCTGTCGGGG
GAAGTGCGGCCGGTGCCCAGTGGCCAGGAGCGGCTCAAGGAAGCGGCCAAGCATGGCTTCAAACGCGCCATCGTGCCCAA
GGGCAATGCGCCGAAGGAAGCGCCGGCGGGGTTGCAGATCATTGCCGTGACGCGCCTGGAACAGGCGCTGGATGCGTTGT
TCGAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(80-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B1YXD7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.015

100

0.492
  radA Streptococcus pneumoniae Rx1

46.304

100

0.468
  radA Streptococcus pneumoniae D39

46.304

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.304

100

0.468
  radA Streptococcus pneumoniae R6

46.304

100

0.468
  radA Streptococcus mitis NCTC 12261

46.087

100

0.466
  radA Streptococcus mitis SK321

46.272

100

0.464

Multiple sequence alignment