Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   STH8232_RS00355 Genome accession   NC_017581
Coordinates   58380..60323 (+) Length   647 a.a.
NCBI ID   WP_014607839.1    Uniprot ID   -
Organism   Streptococcus thermophilus JIM 8232     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 53380..65323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STH8232_RS00325 (STH8232_0066) argR 53584..54012 (+) 429 WP_024704313.1 arginine repressor -
  STH8232_RS00330 (STH8232_0067) - 54012..54371 (+) 360 WP_014621011.1 YlbF family regulator -
  STH8232_RS00335 (STH8232_0068) hexA 54364..56922 (+) 2559 WP_014621012.1 DNA mismatch repair protein MutS Machinery gene
  STH8232_RS00340 (STH8232_0069) - 56956..57411 (-) 456 WP_011680598.1 DUF3021 domain-containing protein -
  STH8232_RS00345 (STH8232_0070) - 57408..57799 (-) 392 Protein_43 LytTR family DNA-binding domain-containing protein -
  STH8232_RS00350 (STH8232_0071) - 58023..58328 (+) 306 WP_014621013.1 hypothetical protein -
  STH8232_RS00355 (STH8232_0072) hexB 58380..60323 (+) 1944 WP_014607839.1 DNA mismatch repair endonuclease MutL Machinery gene
  STH8232_RS00360 (STH8232_0073) - 60453..60674 (+) 222 WP_011680602.1 hypothetical protein -
  STH8232_RS00365 (STH8232_0074) ruvA 60826..61416 (+) 591 WP_011680603.1 Holliday junction branch migration protein RuvA Machinery gene
  STH8232_RS00370 (STH8232_0075) - 61423..61977 (+) 555 WP_014621014.1 DNA-3-methyladenine glycosylase I -
  STH8232_RS00375 (STH8232_0076) cinA 62073..63344 (+) 1272 WP_014621015.1 competence/damage-inducible protein A Machinery gene
  STH8232_RS00380 (STH8232_0077) recA 63380..64519 (+) 1140 WP_011680606.1 recombinase RecA Machinery gene
  STH8232_RS00385 (STH8232_0079) spx 64712..65092 (+) 381 WP_041828975.1 transcriptional regulator Spx -

Sequence

Protein


Download         Length: 647 a.a.        Molecular weight: 72531.80 Da        Isoelectric Point: 5.9934

>NTDB_id=39606 STH8232_RS00355 WP_014607839.1 58380..60323(+) (hexB) [Streptococcus thermophilus JIM 8232]
MPKIIELPEVLANQIAAGEVVERPASVVKELVENAIDAGSTQITIEVEESGLSKIQITDNGEGMAQADVAMSLRRHATSK
IKNQGDLFRIRTLGFRGEALPSIASISHLTIVTAADGEVYGTKLVAKGGEIESQDPISTPVGTKITVENLFYNTPARLKY
MKSLQAELAHIVDVVNRLSLAHPEVAFTLLNDGRQLTQTSGTGDLRQAIAGIYGLTTAKKMVEISNSDLDFEVSGYVSLP
ELTRANRNYITILINGRYIKNFLLNRAIFDGYGSKLMVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKEKELMALIKS
AIAQSLREQDLIPDALENLAKSSTRGATRSVQTSLPLKQTNLYYDSNRNDFFVTPETVQEDIKPLVSKSESSVSLVANKQ
QPTVKQAKRSADDSDSEHGKLDYKNKSKLKRMLENLTNEETSTFPELEFFGQMHGTYLFAQGQGGLYIIDQHAAQERVKY
EYYREKIGVVDSSLQQLLVPYLFEFSGSDYISLQEKMPLLNQVCIYLEPYGNNTFILREHPIWMKEEEIESAVYEMCDML
LLTNEVSVKTYRAELAIMMSCKRSIKANHALDDYSARDLLVQLAQCKNPYNCPHGRPVLVNFTKSDMEKMFRRIQENHTS
LRDLGKY

Nucleotide


Download         Length: 1944 bp        

>NTDB_id=39606 STH8232_RS00355 WP_014607839.1 58380..60323(+) (hexB) [Streptococcus thermophilus JIM 8232]
ATGCCAAAAATTATCGAATTGCCTGAAGTTCTAGCCAATCAGATTGCTGCTGGTGAGGTCGTAGAGCGACCAGCTAGTGT
AGTTAAAGAGCTGGTGGAAAATGCTATTGATGCAGGCAGCACCCAGATTACTATTGAAGTTGAAGAATCAGGCCTCTCAA
AAATTCAAATCACAGACAATGGCGAAGGAATGGCTCAAGCTGATGTAGCTATGAGTTTGCGCCGTCATGCAACGAGTAAA
ATCAAAAATCAAGGAGATCTCTTTCGTATTCGAACACTTGGTTTTCGAGGTGAAGCCCTACCCTCTATAGCTTCTATTAG
TCACCTTACCATTGTGACAGCAGCGGATGGAGAAGTCTATGGGACCAAACTTGTTGCTAAAGGTGGAGAAATTGAGAGTC
AAGATCCTATTTCAACCCCAGTGGGAACAAAGATTACAGTTGAAAACTTGTTTTATAATACCCCAGCTCGTCTCAAGTAT
ATGAAGAGTTTACAGGCTGAGTTGGCTCATATTGTCGATGTTGTCAACCGTTTGAGTTTAGCCCACCCCGAAGTAGCCTT
CACCTTGCTTAATGACGGTCGTCAGTTGACACAGACATCAGGTACTGGAGACCTTCGCCAAGCTATTGCAGGGATTTATG
GCTTAACAACAGCAAAAAAAATGGTGGAAATTTCAAATTCCGACCTTGATTTTGAAGTTTCTGGTTATGTCAGTCTCCCT
GAATTAACGCGTGCTAACCGTAACTATATCACGATTCTTATTAACGGACGCTATATAAAAAACTTCCTCCTTAACCGTGC
TATTTTTGATGGTTATGGTTCTAAACTTATGGTGGGACGCTTCCCAATTGCAGTTATTGATATTCAGATTGATCCTTATT
TGGCAGATGTCAATGTTCACCCAACAAAGCAAGAAGTACGTATCTCTAAAGAAAAGGAGCTCATGGCCCTTATCAAATCT
GCCATTGCTCAAAGCCTTAGAGAGCAGGATTTGATTCCTGATGCCCTTGAAAATCTAGCAAAGTCAAGTACTAGAGGTGC
AACTAGGTCAGTTCAGACGAGTCTCCCACTCAAACAAACCAATCTTTACTATGACAGTAACCGTAATGATTTCTTTGTCA
CGCCAGAGACAGTTCAAGAGGATATCAAACCTCTTGTATCAAAGTCAGAGTCTTCAGTTTCATTGGTAGCAAACAAACAG
CAACCTACTGTTAAACAGGCAAAACGTTCAGCAGATGATAGTGATAGCGAACACGGAAAACTTGACTATAAAAATAAATC
GAAATTGAAGCGCATGCTTGAAAATCTGACCAATGAGGAAACATCAACCTTTCCTGAATTGGAGTTCTTTGGTCAAATGC
ATGGAACCTACCTCTTTGCCCAAGGACAAGGTGGCCTTTACATCATTGACCAGCATGCTGCCCAAGAACGTGTCAAGTAT
GAGTACTATCGCGAAAAGATAGGTGTGGTTGATAGTAGTTTACAACAACTTTTGGTTCCCTATCTTTTTGAATTTTCAGG
TTCAGATTACATTAGTTTACAAGAAAAAATGCCTCTTCTTAACCAAGTTTGCATCTATTTGGAGCCTTATGGGAATAATA
CCTTCATTCTTCGAGAGCACCCTATCTGGATGAAGGAAGAAGAAATCGAATCTGCAGTCTATGAGATGTGTGATATGCTT
CTTTTGACGAACGAAGTATCCGTCAAGACCTATCGTGCTGAGTTAGCCATCATGATGAGTTGTAAACGTTCAATAAAGGC
CAATCATGCTCTTGATGATTACTCTGCGCGTGACCTTCTAGTACAGCTTGCACAGTGTAAAAATCCCTATAATTGTCCTC
ACGGCCGTCCAGTACTTGTCAACTTTACCAAGTCTGATATGGAAAAAATGTTCCGTCGTATTCAAGAAAACCATACCAGC
CTACGTGATTTAGGAAAGTATTAA

Domains

Predicted by InterProScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-124)

MutL C terminal dimerisation domain (MutL_C)

(448-591)

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

69.419

100

0.702

Multiple sequence alignment