Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GFH32_RS01285 Genome accession   NZ_CP045652
Coordinates   302430..303818 (-) Length   462 a.a.
NCBI ID   WP_153509358.1    Uniprot ID   A0A5Q0Q4T3
Organism   Sphingobacterium zhuxiongii strain dk4302     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 297430..308818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH32_RS01265 (GFH32_01265) - 298061..298495 (+) 435 WP_153509356.1 TPM domain-containing protein -
  GFH32_RS01270 (GFH32_01270) - 298500..299336 (+) 837 WP_228384183.1 TPM domain-containing protein -
  GFH32_RS01275 (GFH32_01275) - 299383..300510 (+) 1128 WP_153509357.1 TlpA disulfide reductase family protein -
  GFH32_RS01280 (GFH32_01280) - 300662..302182 (+) 1521 Protein_257 IS1182 family transposase -
  GFH32_RS01285 (GFH32_01285) radA 302430..303818 (-) 1389 WP_153509358.1 DNA repair protein RadA Machinery gene
  GFH32_RS01290 (GFH32_01290) - 303888..304394 (+) 507 WP_153509359.1 RNA polymerase sigma factor -
  GFH32_RS01295 (GFH32_01295) - 304394..305017 (+) 624 WP_153509360.1 hypothetical protein -
  GFH32_RS01300 (GFH32_01300) - 305252..306430 (-) 1179 WP_153509361.1 dipeptidase -
  GFH32_RS01305 (GFH32_01305) - 306430..308160 (-) 1731 WP_153509362.1 serine hydrolase domain-containing protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 50818.07 Da        Isoelectric Point: 6.2716

>NTDB_id=395941 GFH32_RS01285 WP_153509358.1 302430..303818(-) (radA) [Sphingobacterium zhuxiongii strain dk4302]
MAKTKSAYFCQSCGYESPKWLGQCPSCKQWNSFVEEVVEKASSRVPEWRSTSSSKSETKRANKAAIIHEIVYSEEQRIET
PDKEFNRVLGGGIVPGSLVLIGGEPGIGKSTLMLQLALTVSHVKTLYISGEESEQQIKMRAERLVQTSNANCYILTETSM
QNIFKQIDQVQPNLLVIDSIQTLHSAQIESAPGSVSQVRECTAELLRFAKETNTPVFIVGHITKDGSIAGPKVLEHMVDT
VLQFEGDRNHVYRILRAVKNRFGSSSELGIYEMQGSGLREVSNPSEIMLSQREEQVSGTAVAAMLEGMRPLMIEVQALVS
NSAYGTPQRSSTGFDTKRLNMLLAVLEKRFGFRLSAQDVFLNIAGGIRVEDPAIDLAVIAAIISSQQDIPLGSNITFAGE
VGLSGEIRAINRIEQRIQEAEKLGFGQIFISKYNTKGLDAKKYDIAIRPVATLEDVFTALFG

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=395941 GFH32_RS01285 WP_153509358.1 302430..303818(-) (radA) [Sphingobacterium zhuxiongii strain dk4302]
ATGGCAAAAACGAAATCAGCATATTTCTGTCAATCCTGTGGATATGAATCTCCTAAATGGTTGGGACAGTGTCCTTCCTG
CAAACAATGGAACTCATTTGTAGAAGAAGTGGTTGAAAAAGCATCTTCTAGGGTTCCCGAGTGGCGAAGCACATCAAGCT
CAAAAAGCGAAACGAAACGCGCGAATAAAGCTGCGATTATCCACGAAATCGTTTATTCGGAGGAACAGCGCATCGAGACT
CCTGATAAGGAATTTAATCGTGTATTGGGTGGAGGTATTGTTCCAGGGTCTTTAGTGCTCATCGGTGGAGAACCGGGCAT
TGGAAAATCAACGTTGATGCTTCAATTAGCGCTAACCGTCTCCCATGTTAAGACTTTATATATCTCTGGAGAAGAAAGCG
AGCAACAGATTAAAATGCGAGCAGAACGTCTTGTACAGACATCAAACGCGAACTGCTACATCTTGACGGAGACGTCAATG
CAAAATATATTCAAACAGATCGATCAAGTTCAACCGAACTTGTTGGTCATTGATTCGATACAAACCTTGCATTCTGCACA
GATAGAATCAGCACCTGGTTCGGTATCTCAGGTTCGAGAATGTACGGCAGAGCTGCTTCGATTTGCAAAAGAAACGAATA
CACCTGTTTTCATCGTTGGGCATATTACCAAAGATGGTTCAATAGCTGGTCCAAAAGTATTAGAGCATATGGTCGACACG
GTTTTGCAATTTGAGGGCGACAGAAACCACGTTTACCGCATATTACGTGCAGTAAAAAATCGCTTTGGCTCTTCTTCGGA
ATTAGGGATCTATGAAATGCAAGGTTCAGGACTTCGTGAAGTTTCTAATCCTTCGGAAATTATGCTTTCTCAACGGGAGG
AGCAAGTCAGTGGAACCGCTGTTGCAGCCATGTTAGAAGGCATGCGACCATTGATGATTGAAGTACAGGCATTAGTTTCC
AACTCAGCATATGGAACACCGCAACGTAGTTCAACTGGCTTTGACACCAAACGACTGAATATGCTACTTGCAGTCTTGGA
AAAGCGTTTTGGCTTTCGATTAAGCGCGCAAGATGTCTTTTTGAATATTGCTGGAGGTATACGTGTAGAAGATCCTGCGA
TAGATTTAGCGGTTATAGCCGCTATTATTTCTTCTCAACAAGATATTCCTTTAGGTTCCAATATCACTTTTGCGGGGGAA
GTAGGTCTGTCTGGTGAAATACGTGCAATCAATCGTATTGAGCAGCGAATTCAAGAAGCAGAAAAACTAGGATTTGGGCA
AATCTTTATTTCCAAGTACAACACAAAAGGCTTAGACGCGAAAAAGTATGATATCGCTATTCGTCCAGTAGCCACATTGG
AAGACGTATTTACTGCGTTATTCGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(348-434)

AAA domain (AAA_25)

(75-226)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q0Q4T3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

54.279

96.104

0.522
  radA Streptococcus mitis NCTC 12261

51.082

100

0.511
  radA Streptococcus pneumoniae Rx1

51.082

100

0.511
  radA Streptococcus pneumoniae D39

51.082

100

0.511
  radA Streptococcus pneumoniae R6

51.082

100

0.511
  radA Streptococcus pneumoniae TIGR4

51.082

100

0.511
  radA Streptococcus mitis SK321

50.866

100

0.509

Multiple sequence alignment