Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GFH30_RS02305 Genome accession   NZ_CP045650
Coordinates   506174..506962 (+) Length   262 a.a.
NCBI ID   WP_196779986.1    Uniprot ID   -
Organism   Acinetobacter wanghuae strain dk386     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501174..511962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH30_RS02280 (GFH30_02285) - 502110..502961 (+) 852 WP_153370706.1 putative phage abortive infection protein -
  GFH30_RS02285 (GFH30_02290) - 503048..503830 (+) 783 WP_227551545.1 XAC2610-related protein -
  GFH30_RS02290 (GFH30_02295) iscX 504058..504258 (+) 201 WP_153370708.1 Fe-S cluster assembly protein IscX -
  GFH30_RS02295 (GFH30_02300) ndk 504370..504801 (+) 432 WP_035265623.1 nucleoside-diphosphate kinase -
  GFH30_RS02300 (GFH30_02305) rlmN 504924..506147 (+) 1224 WP_153370709.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GFH30_RS02305 (GFH30_02310) pilF 506174..506962 (+) 789 WP_196779986.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GFH30_RS02310 (GFH30_02315) - 506953..507738 (+) 786 WP_153370711.1 DUF4115 domain-containing protein -
  GFH30_RS02315 (GFH30_02320) ispG 507762..508883 (+) 1122 WP_153370712.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GFH30_RS02320 (GFH30_02325) hisS 508887..510182 (+) 1296 WP_153370713.1 histidine--tRNA ligase -
  GFH30_RS02325 (GFH30_02330) - 510212..510913 (+) 702 WP_153370714.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 262 a.a.        Molecular weight: 29538.62 Da        Isoelectric Point: 8.7833

>NTDB_id=395903 GFH30_RS02305 WP_196779986.1 506174..506962(+) (pilF) [Acinetobacter wanghuae strain dk386]
MSQLVLTSTLCVSIALTACQTQDIAKKDPEKAVLVRTQMAVEYLKSGDLDAAKRVLDEALALSSRDSQANMMMGVLLQQE
GSKVNLDKAEGYFKRAIASDPKNAQARNNYGTYLFQVARYNDAIQQFQIAGTTLGYDQRSRALENMGRSYLKLNDVANAE
KSFNQALQANRDSYISMLELAELAYLKQDFPAATRMYEQFVRVVGQKNQNARALWVGIRLARADGDQMGMQVLVNQLRAL
FPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 789 bp        

>NTDB_id=395903 GFH30_RS02305 WP_196779986.1 506174..506962(+) (pilF) [Acinetobacter wanghuae strain dk386]
ATGTCTCAACTTGTTTTAACGTCAACACTTTGTGTGTCTATTGCTTTAACTGCATGTCAAACACAGGATATCGCCAAGAA
AGATCCTGAAAAAGCGGTCTTAGTACGCACCCAAATGGCTGTTGAGTATTTAAAAAGTGGTGACTTGGATGCAGCTAAAC
GTGTTTTAGATGAAGCATTGGCGCTTAGTTCTCGTGATTCTCAAGCCAATATGATGATGGGCGTATTACTGCAACAAGAA
GGCAGTAAGGTCAATCTTGATAAAGCAGAAGGCTACTTTAAACGTGCGATTGCGAGCGATCCAAAGAATGCCCAAGCGCG
CAATAATTACGGCACTTACTTGTTCCAAGTGGCGCGTTATAATGATGCGATCCAACAGTTTCAAATTGCGGGCACAACCT
TGGGTTATGATCAACGCTCACGTGCGCTTGAGAACATGGGACGCAGTTATCTTAAGTTGAATGATGTTGCGAATGCTGAA
AAATCGTTCAACCAAGCCTTACAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAGCTGGCTTATTTAAA
GCAAGATTTCCCCGCTGCGACGCGTATGTATGAACAATTCGTGCGTGTCGTTGGACAAAAAAACCAGAATGCACGTGCAC
TCTGGGTCGGCATACGTCTTGCGCGTGCTGATGGCGATCAGATGGGTATGCAAGTATTGGTGAACCAATTACGTGCTTTG
TTCCCGGAAAGCCAAGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(146-193)

Tetratricopeptide repeat (TPR_16)

(85-129)

Tetratricopeptide repeat (TPR_16)

(39-76)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

72.932

100

0.74
  pilF Acinetobacter baylyi ADP1

67.416

100

0.687

Multiple sequence alignment