Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   HPM96_RS18975 Genome accession   NZ_CP053852
Coordinates   3951460..3952119 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain CE1628     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3946460..3957119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPM96_RS18960 (HPM96_18955) ygiS 3948665..3950272 (-) 1608 WP_001361224.1 ABC transporter substrate-binding protein -
  HPM96_RS18965 (HPM96_18960) ygiV 3950381..3950863 (-) 483 WP_000183492.1 GyrI-like domain-containing protein -
  HPM96_RS18970 (HPM96_18965) ygiW 3950916..3951308 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  HPM96_RS18975 (HPM96_18970) ciaR 3951460..3952119 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  HPM96_RS18980 (HPM96_18975) qseC 3952116..3953465 (+) 1350 WP_063624946.1 quorum sensing histidine kinase QseC -
  HPM96_RS18985 (HPM96_18980) mdaB 3953575..3954156 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  HPM96_RS18990 (HPM96_18985) ygiN 3954187..3954501 (+) 315 WP_000633738.1 putative quinol monooxygenase -
  HPM96_RS18995 (HPM96_18990) - 3954546..3955433 (-) 888 WP_033560346.1 MurR/RpiR family transcriptional regulator -
  HPM96_RS19000 (HPM96_18995) - 3955430..3956377 (-) 948 WP_001305106.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=395899 HPM96_RS18975 WP_001221493.1 3951460..3952119(+) (ciaR) [Escherichia coli strain CE1628]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=395899 HPM96_RS18975 WP_001221493.1 3951460..3952119(+) (ciaR) [Escherichia coli strain CE1628]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTATTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment