Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   HPM97_RS00475 Genome accession   NZ_CP053851
Coordinates   100473..101900 (+) Length   475 a.a.
NCBI ID   WP_074184834.1    Uniprot ID   -
Organism   Escherichia coli strain CE1803A     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 95473..106900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPM97_RS00435 (HPM97_00440) yeeW 95657..95851 (+) 195 WP_000988600.1 DUF5983 family protein -
  HPM97_RS00440 (HPM97_00445) - 95897..95977 (+) 81 Protein_93 hypothetical protein -
  HPM97_RS00445 (HPM97_00450) - 96266..96412 (-) 147 Protein_94 transposase domain-containing protein -
  HPM97_RS00450 (HPM97_00455) - 96466..96648 (+) 183 WP_001016346.1 EutP/PduV family microcompartment system protein -
  HPM97_RS00455 (HPM97_00460) tmaR 96749..97078 (-) 330 WP_000450409.1 DUF496 family protein -
  HPM97_RS00460 (HPM97_00465) yeeA 97250..98308 (-) 1059 WP_001200888.1 FUSC family protein -
  HPM97_RS00465 (HPM97_00470) sbmC 98506..98979 (-) 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
  HPM97_RS00470 (HPM97_00475) dacD 99098..100264 (-) 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  HPM97_RS00475 (HPM97_00480) sbcB 100473..101900 (+) 1428 WP_074184834.1 exodeoxyribonuclease I Machinery gene
  HPM97_RS00480 (HPM97_00485) tsuB 101943..102170 (-) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  HPM97_RS00485 (HPM97_00490) tsuA 102184..103242 (-) 1059 WP_063624967.1 thiosulfate utilization transporter TsuA/YeeE -
  HPM97_RS00490 (HPM97_00495) plaP 103421..104779 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  HPM97_RS00495 (HPM97_00500) yoeI 104769..104831 (-) 63 WP_010723108.1 membrane protein YoeI -
  HPM97_RS00500 (HPM97_00505) yeeY 105046..105975 (-) 930 WP_000803363.1 LysR substrate-binding domain-containing protein -
  HPM97_RS00505 (HPM97_00510) yeeZ 106021..106845 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54527.84 Da        Isoelectric Point: 5.4720

>NTDB_id=395763 HPM97_RS00475 WP_074184834.1 100473..101900(+) (sbcB) [Escherichia coli strain CE1803A]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGENAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=395763 HPM97_RS00475 WP_074184834.1 100473..101900(+) (sbcB) [Escherichia coli strain CE1803A]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGATGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGAGAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCAGGGACGCTGGATTATGCCGAGCAGCAACGTTG
GCTGGAGCACCGCCGCCAGGTATTCACACCCGAGTTTTTACAAGGCTATGCTGATGAATTGCAGATGCTGGTACAACAAT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATCGTCTAG

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583