Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GFB68_RS01600 Genome accession   NZ_CP045605
Coordinates   348314..349687 (+) Length   457 a.a.
NCBI ID   WP_003667336.1    Uniprot ID   A0A2R7DVQ1
Organism   Limosilactobacillus reuteri strain TK-F8A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 343314..354687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFB68_RS01570 (GFB68_01580) - 343590..344339 (+) 750 WP_003667324.1 amino acid ABC transporter ATP-binding protein -
  GFB68_RS01575 (GFB68_01585) - 344355..345146 (+) 792 WP_003667327.1 transporter substrate-binding domain-containing protein -
  GFB68_RS01580 (GFB68_01590) - 345254..346594 (-) 1341 WP_003667329.1 aminopeptidase C -
  GFB68_RS01585 (GFB68_01595) rpiA 346623..347306 (-) 684 WP_003666281.1 ribose-5-phosphate isomerase RpiA -
  GFB68_RS01590 (GFB68_01600) - 347318..347614 (-) 297 WP_011953394.1 GNAT family N-acetyltransferase -
  GFB68_RS01595 (GFB68_01605) - 347753..348289 (+) 537 WP_003667334.1 dUTP diphosphatase -
  GFB68_RS01600 (GFB68_01610) radA 348314..349687 (+) 1374 WP_003667336.1 DNA repair protein RadA Machinery gene
  GFB68_RS01605 (GFB68_01615) - 349706..350857 (+) 1152 WP_003667337.1 PIN/TRAM domain-containing protein -
  GFB68_RS01610 (GFB68_01625) cysS 351232..352644 (+) 1413 WP_003673191.1 cysteine--tRNA ligase -
  GFB68_RS01615 (GFB68_01630) - 352656..353069 (+) 414 WP_003667341.1 Mini-ribonuclease 3 -
  GFB68_RS01620 (GFB68_01635) rlmB 353059..353808 (+) 750 WP_003667343.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GFB68_RS01625 (GFB68_01640) rpmG 353874..354023 (+) 150 WP_003666293.1 50S ribosomal protein L33 -
  GFB68_RS01630 (GFB68_01645) secE 354035..354211 (+) 177 WP_003666294.1 preprotein translocase subunit SecE -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49960.22 Da        Isoelectric Point: 8.1257

>NTDB_id=395597 GFB68_RS01600 WP_003667336.1 348314..349687(+) (radA) [Limosilactobacillus reuteri strain TK-F8A]
MAKVRTQYVCQNCGYNSPRYLGRCPNCGEWNTLVEEQVEANSAPTKKATTTLTGLVAKPQKINEIDSTETPRVKTKLNEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSDEHHRVLYVSGEESGTQIKMRAERLKVAGDDFYVYPETNMDSIR
DTIRDLKPEYVVIDSVQTMQATDVSSAIGSVSQIREVTAQLMQIAKSNNITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMHTNGLTEVKNPSEIFLEERLHDATGSAVVVSLEGTRPILVEIQALVTPTVY
GNAQRTATGLSRNRVSLIMAVLEKRANLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSIASSYRDKGTQPTDAFVGEVGLT
GEIRRVNRIEQRVAEAEKLGFKRIFIPKNNLKGWKPSTNIQVIGVSTLKEALYLALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=395597 GFB68_RS01600 WP_003667336.1 348314..349687(+) (radA) [Limosilactobacillus reuteri strain TK-F8A]
ATGGCAAAAGTTCGAACACAATATGTTTGCCAAAACTGTGGTTACAACTCACCACGCTACTTAGGCCGGTGTCCAAACTG
TGGTGAGTGGAATACTTTAGTAGAAGAACAAGTAGAAGCCAATTCTGCACCAACAAAAAAAGCAACCACCACCTTAACCG
GCTTAGTTGCCAAACCACAGAAAATTAATGAGATTGATAGTACAGAGACTCCCCGCGTAAAAACAAAATTGAACGAGTTA
AATCGTGTTTTAGGTGGGGGAATCGTTCCAGGATCACTAATTCTAATTGGTGGGGATCCTGGAATCGGGAAATCTACCCT
CCTTTTACAAGTTTCTGGACAATTGAGCGATGAGCATCACCGAGTTTTATATGTGTCTGGAGAAGAAAGTGGGACACAAA
TTAAAATGCGGGCGGAACGGTTGAAGGTTGCTGGAGATGACTTTTATGTGTATCCAGAAACGAATATGGATAGTATTCGG
GATACTATTCGTGACCTCAAGCCAGAGTATGTCGTTATTGACTCCGTGCAAACGATGCAGGCAACTGATGTAAGTTCCGC
GATTGGGAGTGTATCGCAAATTCGTGAAGTTACCGCCCAGTTAATGCAAATTGCTAAAAGTAATAACATTACGATTTTTG
TTGTTGGTCATGTGACAAAGGGTGGTGCAATCGCTGGTCCAAAGATTTTAGAGCACATGGTTGACACGGTTTTGTATTTT
GAAGGAGATCTGCACCATACATATCGTATTTTGCGATCAGTGAAAAATCGATTTGGTTCAACCAACGAGCTTGGTATTTT
CGAGATGCATACAAATGGGCTGACGGAAGTAAAAAATCCATCAGAAATCTTTTTAGAGGAACGATTACATGATGCAACTG
GTTCAGCGGTGGTTGTTTCATTAGAAGGAACGCGACCGATTTTAGTGGAAATTCAGGCGCTGGTAACACCGACCGTTTAT
GGAAATGCACAGCGAACTGCTACCGGTTTGAGTCGAAACCGAGTATCATTGATAATGGCAGTATTAGAGAAACGTGCTAA
CCTAATGCTTCAAAATCAAGATGCTTACTTAAAAGCAGCTGGGGGCGTTAAGTTAGATGAGCCAGCGATTGATTTAGCAA
TTGCGGTAAGTATTGCCTCTAGTTACCGGGATAAAGGTACCCAACCAACTGACGCATTTGTCGGTGAAGTAGGTTTAACT
GGGGAAATTCGTCGGGTAAACCGCATTGAACAGCGGGTTGCTGAAGCAGAAAAACTTGGATTTAAACGGATCTTTATTCC
TAAAAATAATTTAAAGGGTTGGAAACCGTCAACTAATATTCAAGTAATTGGTGTCTCAACCCTGAAAGAAGCGTTATATT
TAGCGTTGGGGTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(65-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R7DVQ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.593

98.906

0.659
  radA Streptococcus mitis NCTC 12261

66.593

98.906

0.659
  radA Streptococcus pneumoniae Rx1

66.372

98.906

0.656
  radA Streptococcus pneumoniae D39

66.372

98.906

0.656
  radA Streptococcus pneumoniae R6

66.372

98.906

0.656
  radA Streptococcus pneumoniae TIGR4

66.372

98.906

0.656
  radA Bacillus subtilis subsp. subtilis str. 168

63.02

100

0.63

Multiple sequence alignment