Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   GFB65_RS09865 Genome accession   NZ_CP045598
Coordinates   2101121..2102932 (-) Length   603 a.a.
NCBI ID   WP_002380671.1    Uniprot ID   -
Organism   Enterococcus faecalis strain TK-P4B     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2096121..2107932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFB65_RS09840 (GFB65_10440) - 2097146..2098039 (-) 894 WP_010707216.1 RluA family pseudouridine synthase -
  GFB65_RS09845 (GFB65_10445) - 2098047..2098844 (-) 798 WP_002356486.1 NAD kinase -
  GFB65_RS09850 (GFB65_10450) - 2098857..2099531 (-) 675 WP_002356484.1 GTP pyrophosphokinase family protein -
  GFB65_RS09855 (GFB65_10455) - 2099728..2100306 (+) 579 WP_002356483.1 CYTH domain-containing protein -
  GFB65_RS09860 (GFB65_10460) - 2100370..2101029 (+) 660 WP_002356482.1 ClpXP adapter SpxH family protein -
  GFB65_RS09865 (GFB65_10465) pepF 2101121..2102932 (-) 1812 WP_002380671.1 oligoendopeptidase F Regulator
  GFB65_RS09870 (GFB65_10470) - 2102980..2104158 (-) 1179 Protein_1939 competence protein CoiA -
  GFB65_RS09875 (GFB65_10475) - 2104248..2104910 (-) 663 WP_194848702.1 adaptor protein MecA -
  GFB65_RS09880 (GFB65_10480) spxA 2105253..2105651 (-) 399 WP_002356477.1 transcriptional regulator SpxA -
  GFB65_RS09885 (GFB65_10485) trpS 2106011..2107021 (+) 1011 WP_002378619.1 tryptophan--tRNA ligase -

Sequence

Protein


Download         Length: 603 a.a.        Molecular weight: 68849.19 Da        Isoelectric Point: 4.7490

>NTDB_id=395531 GFB65_RS09865 WP_002380671.1 2101121..2102932(-) (pepF) [Enterococcus faecalis strain TK-P4B]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLTEELNQAESFKGTLGNGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALEKYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide


Download         Length: 1812 bp        

>NTDB_id=395531 GFB65_RS09865 WP_002380671.1 2101121..2102932(-) (pepF) [Enterococcus faecalis strain TK-P4B]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTAACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGACAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGAACCTTAGGGA
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATAGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGACCCAGAGGTTTTAACATTAAGTGATGAACAAATCTGGGGATATTTTGAAGAGCAGC
CCAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAGTTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGTTTGTACAAAGTATACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGAGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTGAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGTGGCGCATATTCTTCAGGAGCTTATGACACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGCGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACCCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACAAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAAGGAGCTTTAGAGAAGTACCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAG

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(207-587)

Oligopeptidase F (Peptidase_M3_N)

(117-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

54.637

98.342

0.537

Multiple sequence alignment