Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GFB63_RS03220 Genome accession   NZ_CP045596
Coordinates   643139..644500 (-) Length   453 a.a.
NCBI ID   WP_080998440.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain TK-P3A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 638139..649500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFB63_RS03190 (GFB63_03275) rplA 639885..640574 (-) 690 WP_024704232.1 50S ribosomal protein L1 -
  GFB63_RS03195 (GFB63_03280) rplK 640674..641099 (-) 426 WP_002953535.1 50S ribosomal protein L11 -
  GFB63_RS03200 (GFB63_03285) - 641271..641624 (+) 354 WP_002953536.1 DUF3397 domain-containing protein -
  GFB63_RS03205 (GFB63_03290) - 641716..642213 (-) 498 WP_002947059.1 carbonic anhydrase -
  GFB63_RS10505 - 642356..643050 (-) 695 Protein_648 TIGR00266 family protein -
  GFB63_RS03220 (GFB63_03305) radA 643139..644500 (-) 1362 WP_080998440.1 DNA repair protein RadA Machinery gene
  GFB63_RS03225 (GFB63_03310) - 644678..645124 (-) 447 WP_002947069.1 dUTP diphosphatase -
  GFB63_RS03230 (GFB63_03315) - 645338..645941 (+) 604 Protein_651 NADPH-dependent FMN reductase -
  GFB63_RS03235 (GFB63_03320) - 645960..647213 (+) 1254 WP_193838378.1 NAD(P)H-dependent oxidoreductase -
  GFB63_RS03240 (GFB63_03325) - 647331..648122 (+) 792 Protein_653 formate/nitrite transporter family protein -
  GFB63_RS11045 (GFB63_03330) - 648131..648703 (+) 573 Protein_654 MptD family putative ECF transporter S component -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49418.80 Da        Isoelectric Point: 6.6528

>NTDB_id=395456 GFB63_RS03220 WP_080998440.1 643139..644500(-) (radA) [Streptococcus thermophilus strain TK-P3A]
MAKKKSTFVCRECGYNSPKYLGRCPNCSSWSSFEEEVEVQEVKNARVSLTGEKSKPTKLKEVSSISYARIKTDMDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQAVRSQVE
GIKPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELIQIAKTNNIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAIVVTMEGSRPILAEVQALVTPTVFGNAK
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKELPTNPQEAFVGEIGLTGEIR
RVTRIEQRINEAAKLGFTKIYVPKNSLHGMKIPEGIQVIGVTTVGEVLKKVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=395456 GFB63_RS03220 WP_080998440.1 643139..644500(-) (radA) [Streptococcus thermophilus strain TK-P3A]
ATAGCTAAGAAAAAATCAACTTTTGTTTGTAGAGAATGTGGCTACAATTCACCCAAATATCTAGGGCGCTGTCCCAATTG
TTCTTCTTGGTCCTCTTTTGAGGAAGAGGTTGAGGTGCAGGAGGTTAAAAATGCCCGTGTCAGTTTGACGGGTGAAAAGT
CCAAACCTACTAAGTTAAAAGAAGTCTCTTCAATTAGTTATGCCCGCATAAAAACCGATATGGACGAGTTTAACCGTGTT
TTAGGTGGCGGTGTGGTTCCGGGGAGTTTGGTTCTTATTGGAGGAGATCCAGGAATAGGGAAATCGACCCTCCTCTTACA
GGTGTCAACACAGCTGGCTAACAAGGGAACTGTCCTCTATGTTTCTGGGGAAGAATCGGCCGAGCAAATCAAATTGCGGA
GTGAACGTTTAGGTGATATTGATAACGAATTTTATCTGTATGCTGAGACCAATATGCAAGCAGTCCGTAGTCAAGTGGAG
GGTATCAAACCCGATTTCCTCATTATTGATTCTATTCAGACCATCATGAGCCCAGAGATCTCTGGCGTTCAAGGTTCTGT
TTCCCAGGTTCGTGAAGTAACTGCCGAACTCATACAGATTGCCAAAACCAATAACATTGCCACTTTTATTGTTGGGCATG
TGACCAAGGAGGGACAGCTGGCTGGACCACGTATGTTGGAGCACATGGTGGATACTGTGCTTTATTTTGAGGGGGAACGT
CATCATACCTTCCGTATTCTGAGGGCAGTTAAAAACCGTTTTGGTTCAACCAATGAGATTGGGATTTTTGAAATGCAGTC
AGGTGGTCTGGTCGAGGTACTAAACCCTAGCCAAGTTTTCTTAGAAGAACGCCTAGACGGTGCCACAGGTTCTGCCATTG
TTGTCACAATGGAGGGGAGTCGACCTATCCTAGCTGAGGTTCAAGCTTTGGTGACACCGACCGTCTTTGGTAACGCAAAG
CGGACAACGACGGGACTGGACTTTAACCGTGTCAGCCTTATCATGGCTGTCTTAGAGAAACGTTGCGGCCTTCTTCTCCA
AAATCAAGATGCCTATCTCAAATCAGCTGGTGGTGTCAAGTTGGACGAGCCAGCTATTGACCTTGCAGTTGCCGTTGCTA
TCGCTTCTAGTTACAAGGAGCTTCCAACCAATCCACAAGAAGCCTTCGTAGGAGAAATTGGCTTGACCGGAGAGATTCGT
CGTGTGACTCGTATTGAGCAGCGTATCAATGAAGCCGCTAAACTAGGTTTTACTAAGATTTATGTTCCTAAAAATTCGCT
TCATGGCATGAAGATTCCAGAAGGTATCCAGGTCATTGGGGTCACAACGGTAGGAGAAGTACTCAAAAAAGTCTTTTCTT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

88.962

100

0.89
  radA Streptococcus pneumoniae D39

88.962

100

0.89
  radA Streptococcus pneumoniae R6

88.962

100

0.89
  radA Streptococcus pneumoniae TIGR4

88.962

100

0.89
  radA Streptococcus mitis NCTC 12261

88.962

100

0.89
  radA Streptococcus mitis SK321

88.962

100

0.89
  radA Bacillus subtilis subsp. subtilis str. 168

61.148

100

0.611

Multiple sequence alignment