Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GFB61_RS01710 Genome accession   NZ_CP045590
Coordinates   334147..335523 (+) Length   458 a.a.
NCBI ID   WP_014918250.1    Uniprot ID   A0A3S8SE54
Organism   Lactobacillus helveticus strain TK-J7A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 329147..340523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFB61_RS01690 (GFB61_01720) pepC 330094..331443 (-) 1350 WP_003625856.1 aminopeptidase C -
  GFB61_RS01695 (GFB61_01725) - 331754..333013 (-) 1260 WP_107775328.1 RNA-guided endonuclease TnpB family protein -
  GFB61_RS01700 (GFB61_01735) - 333158..333460 (-) 303 WP_003625857.1 hypothetical protein -
  GFB61_RS01705 (GFB61_01740) - 333596..334147 (+) 552 WP_012211405.1 dUTP diphosphatase -
  GFB61_RS01710 (GFB61_01745) radA 334147..335523 (+) 1377 WP_014918250.1 DNA repair protein RadA Machinery gene
  GFB61_RS01715 (GFB61_01750) gltX 335599..337098 (+) 1500 WP_003625860.1 glutamate--tRNA ligase -
  GFB61_RS01720 (GFB61_01755) cysS 337191..338621 (+) 1431 WP_041808634.1 cysteine--tRNA ligase -
  GFB61_RS01725 (GFB61_01760) - 338614..339054 (+) 441 WP_014918252.1 Mini-ribonuclease 3 -
  GFB61_RS01730 (GFB61_01765) rlmB 339041..339793 (+) 753 WP_003625865.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GFB61_RS01735 (GFB61_01770) - 339936..340481 (+) 546 WP_014918253.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50211.64 Da        Isoelectric Point: 6.0792

>NTDB_id=395401 GFB61_RS01710 WP_014918250.1 334147..335523(+) (radA) [Lactobacillus helveticus strain TK-J7A]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGLNEPVHLDKIKAEKEERIPTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLHIMSDLAEKYKVLYVSGEESANQIKLRADRLGVGQSNMLLYPETDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHTYRILHSVKNRFGAANEIGMFEMENEGLREVTNPSSIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGISFNKASLLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMSVASSYTDKEISPTDCFVGEVGL
TGEVRSVDKIEARVKEAAKVGFKRIFIPKHNMYQSLTENGIEVIPVASIPQALELVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=395401 GFB61_RS01710 WP_014918250.1 334147..335523(+) (radA) [Lactobacillus helveticus strain TK-J7A]
ATGGCTAGAGTAAAAACGCAATATAAATGTCGTTCCTGTGGTTATATTTCAGCTAGTTACTTGGGTCGTTGTCCTAACTG
CGGTGCATGGAATCAATTTGAAAAAGAAACTGAAGAAGTACAGAAAAGATCTACTAAAGCAACGGCAAGCCGTTTAATTC
AAAAAACAGGTCTTAATGAACCTGTTCATTTGGATAAAATCAAGGCTGAAAAAGAAGAACGAATTCCTACTAAGTCCGAA
GAATTAAATCGTGTATTAGGTGGCGGGATCGTTCCTGGATCCCTCGTCTTAATTGGTGGGGACCCTGGTATTGGTAAGTC
AACTTTAATGTTGCATATTATGAGTGATCTGGCAGAAAAATATAAAGTGCTTTATGTTTCAGGAGAAGAGTCAGCTAATC
AAATCAAGCTGAGGGCAGATCGGCTTGGAGTTGGTCAAAGCAACATGCTTTTATATCCAGAAACAGATATGCATGATATT
CGTGAGCAGATCAATGATGTTAAGCCTGATTTTGTCGTTATTGATTCTATTCAAACGATGAATGAACCTAGTCTTGATTC
AATGACTGGTTCTGCTTCACAAGTACGTGAGGTAACAAGTGAATTGATGAAGATTGCTAAGATGGATGCAATTACTGTTT
TTGTTATTGGACACGTGACTAAAGAAGGTGCGATTGCTGGCCCCAAAATTTTGGAACACATGGTGGATACTGTCCTTTAT
TTTGAGGGGGATGAACACCACACGTACCGGATTTTGCATTCCGTAAAAAATCGTTTTGGTGCTGCTAACGAAATCGGCAT
GTTTGAAATGGAAAATGAAGGCTTACGTGAAGTAACCAATCCATCTTCGATTTTCCTTGATCAAAGATTACCTAATTCAA
CTGGGTCAGCCGTAGTAGTTTCACTTGAAGGAACGCGGCCACTTTTGGCAGAAATTCAAGCATTAGTAACGCCAACTGCA
TTTGGCTATGCCAAACGAACTACTTCAGGAATTAGTTTTAATAAAGCTTCACTATTATTAGCGGTTCTTGAAAAACGCGG
CAATTTAATGCTTCAAAATCAGGATGTTTATTTAACGGCTACTGGCGGTATTAAATTAAATGAACCTGCAATCGATCTAG
CTATTGCTATGTCAGTAGCATCAAGCTACACCGATAAAGAAATCTCACCGACTGATTGTTTTGTAGGGGAAGTGGGATTA
ACCGGTGAAGTTCGCAGCGTTGATAAAATCGAAGCTAGAGTAAAGGAAGCTGCCAAGGTAGGTTTTAAACGTATTTTTAT
TCCTAAGCACAATATGTATCAAAGTCTAACGGAAAATGGCATTGAAGTAATTCCAGTTGCCAGCATTCCACAGGCATTAG
AATTAGTTTTTGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)

AAA domain (AAA_25)

(66-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S8SE54

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

56.893

99.782

0.568
  radA Streptococcus pneumoniae Rx1

56.893

99.782

0.568
  radA Streptococcus pneumoniae D39

56.893

99.782

0.568
  radA Streptococcus pneumoniae R6

56.893

99.782

0.568
  radA Streptococcus pneumoniae TIGR4

56.893

99.782

0.568
  radA Streptococcus mitis SK321

56.893

99.782

0.568
  radA Bacillus subtilis subsp. subtilis str. 168

55.773

100

0.559

Multiple sequence alignment