Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   GFH33_RS27945 Genome accession   NZ_CP045585
Coordinates   5354888..5356237 (-) Length   449 a.a.
NCBI ID   WP_000879427.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain x023     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5349888..5361237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH33_RS27910 (GFH33_27910) - 5349932..5350627 (-) 696 WP_000466508.1 hypothetical protein -
  GFH33_RS27915 (GFH33_27915) - 5350614..5351402 (-) 789 WP_000120048.1 hypothetical protein -
  GFH33_RS27920 (GFH33_27920) - 5351430..5351999 (-) 570 WP_001211058.1 hypothetical protein -
  GFH33_RS27925 (GFH33_27925) - 5352117..5352590 (-) 474 WP_000511547.1 hypothetical protein -
  GFH33_RS27930 (GFH33_27930) raiA 5352995..5353537 (-) 543 WP_000671186.1 ribosome-associated translation inhibitor RaiA -
  GFH33_RS27935 (GFH33_27935) cspC 5353860..5354057 (-) 198 WP_001990088.1 cold shock protein CspC -
  GFH33_RS27940 (GFH33_27940) - 5354184..5354888 (-) 705 WP_016100030.1 ComF family protein -
  GFH33_RS27945 (GFH33_27945) comFA 5354888..5356237 (-) 1350 WP_000879427.1 ATP-dependent helicase ComFA Machinery gene
  GFH33_RS27950 (GFH33_27950) - 5356365..5357777 (-) 1413 WP_000499473.1 C40 family peptidase -
  GFH33_RS27955 (GFH33_27955) - 5357926..5358240 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  GFH33_RS27960 (GFH33_27960) - 5358413..5359255 (-) 843 WP_002101558.1 DegV family protein -
  GFH33_RS27965 (GFH33_27965) - 5359492..5360127 (+) 636 WP_000926665.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51000.91 Da        Isoelectric Point: 9.9654

>NTDB_id=395351 GFH33_RS27945 WP_000879427.1 5354888..5356237(-) (comFA) [Bacillus thuringiensis strain x023]
MLAGKQLLLEELSSDLQRELNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCAYCRKCITMGRVS
ECAVLVRGIAERKREKNSNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKVAAFKKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEMFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=395351 GFH33_RS27945 WP_000879427.1 5354888..5356237(-) (comFA) [Bacillus thuringiensis strain x023]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGGAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCGCATATTGCCGGAAGTGTATAACGATGGGGAGAGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTCAAATTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACTACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCGTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCATCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCTATTTTTTTA
TTCGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCAGGGAGAAAAGAAAAAGTAGCGGCTTTCAAAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTCGCAGTTTTGGGGGCGGAAGAAGAAATGTTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.905

98.441

0.501
  comFA Latilactobacillus sakei subsp. sakei 23K

41.191

89.755

0.37

Multiple sequence alignment