Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   GFH33_RS22990 Genome accession   NZ_CP045585
Coordinates   4447110..4447856 (-) Length   248 a.a.
NCBI ID   WP_000487013.1    Uniprot ID   A0A9W5QDK4
Organism   Bacillus thuringiensis strain x023     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4442110..4452856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH33_RS22970 (GFH33_22970) dnaG 4442871..4444667 (-) 1797 WP_000528222.1 DNA primase -
  GFH33_RS22975 (GFH33_22975) - 4444749..4444892 (-) 144 WP_000479733.1 hypothetical protein -
  GFH33_RS22980 (GFH33_22980) - 4445528..4446340 (-) 813 WP_000368936.1 pyruvate, water dikinase regulatory protein -
  GFH33_RS22985 (GFH33_22985) - 4446370..4447002 (-) 633 WP_000583756.1 helix-turn-helix transcriptional regulator -
  GFH33_RS22990 (GFH33_22990) recO 4447110..4447856 (-) 747 WP_000487013.1 DNA repair protein RecO Machinery gene
  GFH33_RS22995 (GFH33_22995) - 4447890..4448033 (-) 144 WP_000883924.1 YqzL family protein -
  GFH33_RS23000 (GFH33_23000) era 4448210..4449115 (-) 906 WP_001080229.1 GTPase Era -
  GFH33_RS23005 (GFH33_23005) - 4449108..4449506 (-) 399 WP_001086282.1 cytidine deaminase -
  GFH33_RS23010 (GFH33_23010) - 4449606..4449959 (-) 354 WP_000715469.1 diacylglycerol kinase family protein -
  GFH33_RS23015 (GFH33_23015) ybeY 4449956..4450426 (-) 471 WP_000054679.1 rRNA maturation RNase YbeY -
  GFH33_RS23020 (GFH33_23020) - 4450423..4452567 (-) 2145 WP_000091514.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28609.20 Da        Isoelectric Point: 8.0488

>NTDB_id=395341 GFH33_RS22990 WP_000487013.1 4447110..4447856(-) (recO) [Bacillus thuringiensis strain x023]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGQFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=395341 GFH33_RS22990 WP_000487013.1 4447110..4447856(-) (recO) [Bacillus thuringiensis strain x023]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAACTTGGTAAGGTAAGTGCAATGGCAAGAGGGGCAAAAAAACCGAAAAGCCGATTAGCATCTGTGTCGCAATTAATGA
CACATGGCCAGTTTCTTATTCAAATGGGATCTGGGCTTGGAACATTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAATTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAC
TTTGTCGCCTTCTCCGTTAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCGGAGCAAGACCCGTATCGTATACCGGTAGG
AGAAGCTGTTCATAAATTATTACGTCTTTTCTTCCATTTCGATTTACATAGGCTCGGAAATGTATCGGTAAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTTTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment