Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GFH33_RS21455 Genome accession   NZ_CP045585
Coordinates   4175283..4175717 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain x023     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4170283..4180717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH33_RS21440 (GFH33_21440) - 4171461..4172018 (+) 558 WP_000062063.1 PadR family transcriptional regulator -
  GFH33_RS21445 (GFH33_21445) metE 4172530..4174818 (+) 2289 WP_001007605.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  GFH33_RS21450 (GFH33_21450) comJ 4174857..4175246 (-) 390 WP_000424030.1 competence protein ComJ -
  GFH33_RS21455 (GFH33_21455) nucA/comI 4175283..4175717 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  GFH33_RS21460 (GFH33_21460) - 4175944..4176693 (+) 750 WP_000388472.1 DUF3967 domain-containing protein -
  GFH33_RS21465 (GFH33_21465) kinB 4176732..4178006 (-) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  GFH33_RS21470 (GFH33_21470) - 4178317..4178517 (+) 201 WP_000929277.1 hypothetical protein -
  GFH33_RS21475 (GFH33_21475) - 4178640..4179803 (+) 1164 WP_000434578.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=395337 GFH33_RS21455 WP_000811494.1 4175283..4175717(-) (nucA/comI) [Bacillus thuringiensis strain x023]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=395337 GFH33_RS21455 WP_000811494.1 4175283..4175717(-) (nucA/comI) [Bacillus thuringiensis strain x023]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGTGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment