Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   GFH33_RS09280 Genome accession   NZ_CP045585
Coordinates   1716219..1716821 (-) Length   200 a.a.
NCBI ID   WP_000155598.1    Uniprot ID   A0A9W5VGG2
Organism   Bacillus thuringiensis strain x023     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1711219..1721821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH33_RS09260 (GFH33_09260) dnaD 1711534..1712241 (+) 708 WP_000728552.1 DNA replication protein DnaD -
  GFH33_RS09265 (GFH33_09265) nth 1712257..1712904 (+) 648 WP_000933034.1 endonuclease III -
  GFH33_RS09270 (GFH33_09270) - 1712908..1713426 (+) 519 WP_000442652.1 YpoC family protein -
  GFH33_RS09275 (GFH33_09275) - 1713453..1716152 (-) 2700 WP_001283099.1 PBP1A family penicillin-binding protein -
  GFH33_RS09280 (GFH33_09280) recU 1716219..1716821 (-) 603 WP_000155598.1 Holliday junction resolvase RecU Machinery gene
  GFH33_RS09285 (GFH33_09285) - 1716925..1717950 (+) 1026 WP_000495649.1 DUF2515 domain-containing protein -
  GFH33_RS32245 - 1718102..1718281 (-) 180 WP_001987054.1 hypothetical protein -
  GFH33_RS09290 (GFH33_09290) - 1718367..1718609 (+) 243 WP_001252036.1 hypothetical protein -
  GFH33_RS09295 (GFH33_09295) - 1718625..1718969 (+) 345 WP_000786942.1 YppE family protein -
  GFH33_RS09300 (GFH33_09300) - 1718996..1719187 (-) 192 WP_000242652.1 YppF family protein -
  GFH33_RS09305 (GFH33_09305) - 1719365..1719874 (+) 510 WP_000487624.1 YppG family protein -
  GFH33_RS09310 (GFH33_09310) - 1720030..1720458 (+) 429 WP_000546268.1 CotD family spore coat protein -
  GFH33_RS09315 (GFH33_09315) - 1720515..1721069 (+) 555 WP_000862914.1 DUF1273 domain-containing protein -
  GFH33_RS09320 (GFH33_09320) gpsB 1721168..1721503 (+) 336 WP_000622431.1 cell division regulator GpsB -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23355.63 Da        Isoelectric Point: 9.4336

>NTDB_id=395327 GFH33_RS09280 WP_000155598.1 1716219..1716821(-) (recU) [Bacillus thuringiensis strain x023]
MTIRYPNGKRYNQASQPQKTPIKTHTYSNRGMSLEEELNETNQYYLTHNIACVHKKPTPLQIVKVDYPARSAAVVKEAYF
KQPSTTDYNGVYKGKYIDFEAKETKNKTSFPLQNFHLHQIEHMKQVVAHNGIAFVIIKFTLFDEFYLLDAKHIIAFWNRQ
NTGGRKSITKEEIEEHGSLLSCGYHPRIDYIRVLDMVYFS

Nucleotide


Download         Length: 603 bp        

>NTDB_id=395327 GFH33_RS09280 WP_000155598.1 1716219..1716821(-) (recU) [Bacillus thuringiensis strain x023]
ATGACCATTCGTTACCCAAATGGAAAAAGGTATAATCAAGCTTCACAACCTCAAAAAACACCTATAAAAACACATACTTA
TAGTAATAGAGGTATGTCCCTTGAAGAGGAATTGAATGAAACAAATCAATATTACTTAACCCATAATATTGCATGTGTAC
ATAAAAAGCCTACACCTCTTCAAATTGTAAAAGTAGATTACCCCGCTCGAAGTGCTGCAGTGGTAAAAGAAGCGTATTTT
AAACAACCTTCTACAACAGATTACAACGGTGTATACAAAGGGAAATACATCGATTTTGAAGCAAAGGAAACCAAAAATAA
AACTAGTTTCCCACTTCAAAACTTCCACCTTCATCAAATTGAACATATGAAGCAAGTCGTTGCTCATAATGGAATTGCAT
TTGTTATTATTAAATTTACACTTTTTGATGAATTTTATTTACTAGATGCAAAACATATTATTGCATTTTGGAATCGTCAA
AATACTGGTGGACGCAAGTCGATTACGAAAGAAGAAATAGAAGAGCATGGATCCTTATTATCATGCGGTTATCACCCTCG
GATTGACTATATCCGTGTACTAGACATGGTTTATTTTTCGTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(30-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

63.5

100

0.635

Multiple sequence alignment