Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GE569_RS08315 Genome accession   NZ_CP045576
Coordinates   1825076..1826452 (+) Length   458 a.a.
NCBI ID   WP_186048201.1    Uniprot ID   -
Organism   Burkholderia gladioli strain KACC 18963     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1820076..1831452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GE569_RS08295 (GE569_08275) - 1820528..1821484 (+) 957 WP_186110728.1 DUF1853 family protein -
  GE569_RS08300 (GE569_08280) thiD 1821563..1822375 (-) 813 WP_096748625.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  GE569_RS08305 (GE569_08285) lplT 1822476..1823783 (-) 1308 WP_096748626.1 lysophospholipid transporter LplT -
  GE569_RS08310 (GE569_08290) alr 1823995..1825065 (+) 1071 WP_013698534.1 alanine racemase -
  GE569_RS08315 (GE569_08295) radA 1825076..1826452 (+) 1377 WP_186048201.1 DNA repair protein RadA Machinery gene
  GE569_RS08320 (GE569_08300) - 1826584..1826847 (+) 264 WP_013698532.1 DUF2866 domain-containing protein -
  GE569_RS08325 (GE569_08305) - 1826987..1828912 (-) 1926 WP_186110729.1 ATP-binding cassette domain-containing protein -
  GE569_RS08330 (GE569_08310) - 1829103..1829588 (+) 486 WP_036050850.1 glutathione peroxidase -
  GE569_RS08335 (GE569_08315) cls 1829620..1831059 (-) 1440 WP_096748630.1 cardiolipin synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 48714.12 Da        Isoelectric Point: 7.2038

>NTDB_id=395298 GE569_RS08315 WP_186048201.1 1825076..1826452(+) (radA) [Burkholderia gladioli strain KACC 18963]
MAKQKTVYICTECGGQSLKWQGQCPSCHAWNTLVESAAETPSQHRFQALAKSAPVQRLADIDAADVPRFSTGIGEFDRVL
GGGLVAGGVVLIGGDPGIGKSTLLLQSLAQLASQRRALYISGEESAAQIALRAQRLALLDHGGDAADLKLLAEIQLEKIQ
ATIAAERPDVAVIDSIQTVYSEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGALAGPRVLEHIVDTVLYF
EGDTHSSYRLVRAFKNRFGAVNELGVFAMTERGLRGVSNPSALFLSQHEEVVPGSCVLVTQEGSRPLLVEVQALVDTANV
PNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKALPKGLVVFGEVGLA
GEIRPSPRGQERLREAAKLGFTIALIPKANAPKQPIEGLTVMAVERIEQAIDRVRGLE

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=395298 GE569_RS08315 WP_186048201.1 1825076..1826452(+) (radA) [Burkholderia gladioli strain KACC 18963]
GTGGCGAAACAGAAAACGGTCTATATCTGCACCGAATGCGGCGGCCAGTCGCTGAAATGGCAGGGCCAGTGCCCGTCCTG
CCATGCCTGGAACACGCTGGTCGAATCGGCCGCCGAAACCCCTTCGCAGCACCGCTTCCAGGCGCTCGCCAAGAGCGCGC
CGGTGCAGCGCCTGGCCGATATCGACGCGGCCGACGTGCCGCGCTTTTCCACCGGCATCGGCGAGTTCGACCGCGTGCTC
GGCGGCGGCCTGGTGGCCGGCGGCGTGGTGCTGATCGGCGGCGACCCCGGCATCGGCAAATCCACCCTGTTGCTGCAGTC
GCTGGCCCAGCTGGCCAGCCAGCGCCGCGCGCTCTATATCAGCGGCGAGGAATCGGCCGCCCAGATCGCCCTGCGCGCCC
AGCGCCTGGCCCTGCTCGATCACGGCGGCGACGCCGCCGATCTCAAGCTGCTGGCGGAAATCCAGCTCGAGAAGATCCAG
GCCACGATCGCCGCCGAGCGGCCCGACGTGGCCGTGATCGACTCGATCCAGACCGTCTATTCGGAAGCGCTCACCTCGGC
GCCCGGCTCGGTGGCCCAGGTGCGCGAATGCGCGGCCCAGCTCACGCGCATCGCCAAGCAGTCGGGCACCGCGATCATCA
TGGTCGGCCACGTCACCAAGGAGGGCGCGCTGGCGGGCCCGCGCGTGCTGGAGCACATCGTCGACACGGTGCTGTACTTC
GAAGGCGACACGCATTCTTCCTACCGGCTGGTGCGCGCCTTCAAGAACCGCTTCGGCGCGGTCAACGAGCTCGGGGTGTT
CGCGATGACTGAGCGCGGCCTGCGCGGCGTGTCCAACCCGTCCGCGCTGTTCCTCTCGCAGCACGAGGAGGTGGTGCCGG
GTTCCTGCGTGCTGGTCACGCAGGAGGGCTCGCGGCCGCTGCTGGTGGAGGTGCAGGCGCTGGTCGACACCGCCAACGTG
CCGAATCCGCGGCGCCTGGCGGTGGGCCTGGAGCAGAACCGGCTGGCCATGCTGCTGGCCGTGCTGCACCGCCATGCGGG
CATCGCCTGCTTCGACCAGGACGTGTTCCTGAACGCGGTGGGGGGCGTCAAGATTACCGAGCCGGCCGCTGACCTGGCGG
TGCTGCTGGCGATCCATTCGTCGATGCGTAACAAAGCGTTGCCGAAGGGCCTGGTGGTGTTCGGCGAGGTCGGGCTGGCC
GGCGAGATCCGGCCTTCGCCGCGCGGCCAGGAGCGTCTGCGCGAGGCGGCCAAGCTGGGTTTCACGATCGCGCTGATTCC
CAAGGCCAATGCGCCCAAGCAGCCGATCGAGGGCCTCACCGTGATGGCCGTCGAGCGCATCGAGCAGGCCATCGACCGCG
TGCGCGGCCTCGAATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.707

100

0.493
  radA Streptococcus pneumoniae Rx1

46.32

100

0.467
  radA Streptococcus pneumoniae D39

46.32

100

0.467
  radA Streptococcus pneumoniae R6

46.32

100

0.467
  radA Streptococcus pneumoniae TIGR4

46.32

100

0.467
  radA Streptococcus mitis SK321

46.507

100

0.465
  radA Streptococcus mitis NCTC 12261

46.171

99.782

0.461

Multiple sequence alignment