Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GE559_RS09360 Genome accession   NZ_CP045573
Coordinates   2017312..2018817 (+) Length   501 a.a.
NCBI ID   WP_013699407.1    Uniprot ID   A0A560VPW2
Organism   Burkholderia gladioli strain KACC 18962     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2012312..2023817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GE559_RS09335 (GE559_09260) kdsD 2013521..2014504 (+) 984 WP_186110957.1 arabinose 5-phosphate isomerase KdsD -
  GE559_RS09340 (GE559_09265) - 2014504..2015043 (+) 540 WP_098153593.1 HAD family hydrolase -
  GE559_RS09345 (GE559_09270) lptC 2015047..2015661 (+) 615 WP_017918294.1 LPS export ABC transporter periplasmic protein LptC -
  GE559_RS09350 (GE559_09275) lptA 2015680..2016348 (+) 669 WP_013699405.1 lipopolysaccharide transport periplasmic protein LptA -
  GE559_RS09355 (GE559_09280) lptB 2016345..2017121 (+) 777 WP_013699406.1 LPS export ABC transporter ATP-binding protein -
  GE559_RS09360 (GE559_09285) rpoN/rpoN1 2017312..2018817 (+) 1506 WP_013699407.1 RNA polymerase factor sigma-54 Machinery gene
  GE559_RS09365 (GE559_09290) raiA 2018968..2019333 (+) 366 WP_013699408.1 ribosome-associated translation inhibitor RaiA -
  GE559_RS09370 (GE559_09295) - 2019548..2020060 (+) 513 WP_096750181.1 PTS sugar transporter subunit IIA -
  GE559_RS09375 (GE559_09300) hprK 2020186..2021154 (+) 969 WP_013699410.1 HPr(Ser) kinase/phosphatase -
  GE559_RS09380 (GE559_09305) rapZ 2021265..2022158 (+) 894 WP_013699411.1 RNase adapter RapZ -
  GE559_RS09385 (GE559_09310) - 2022191..2022826 (+) 636 WP_013699412.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55047.01 Da        Isoelectric Point: 4.9483

>NTDB_id=395287 GE559_RS09360 WP_013699407.1 2017312..2018817(+) (rpoN/rpoN1) [Burkholderia gladioli strain KACC 18962]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDEWIASPLRVAADGSLIAQSPPSSTPEPMHG
GGSNGSEASGDRAERDDSRNGDDYDYNADSGDSGQWNLDDYGRSSGASDDDDLPPLQVHEASTSLREHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLGAGLDEVLTDLPEELEVDLDELGAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTRTL
SLDIVSQHLELLAARDFTRLRKQLKANDDELREAHALIRSLEPFPGAAYGKTEADYVVPDIMVRKSGQNWLAELNPEVVP
KLRINHLYANILRNSRGDPGAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDQKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=395287 GE559_RS09360 WP_013699407.1 2017312..2018817(+) (rpoN/rpoN1) [Burkholderia gladioli strain KACC 18962]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCACCTCGCGCTCACGCCCCAGCTCCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCCCAGAATCCGCTGCTCGAGAACGACGAATGGATCG
CGAGCCCGCTGCGGGTGGCCGCCGACGGCTCGCTGATCGCGCAGAGCCCGCCTTCCTCGACGCCCGAGCCGATGCACGGC
GGCGGCTCGAACGGCAGCGAAGCCTCGGGCGATCGCGCCGAACGCGACGACTCGCGCAATGGCGACGACTACGACTACAA
CGCCGACAGCGGCGATTCGGGTCAGTGGAATCTCGACGACTACGGCCGCTCCTCGGGCGCCTCCGACGATGACGACCTCC
CGCCGCTGCAGGTGCACGAGGCCTCGACCTCGCTGCGCGAGCATCTGTCCGCGCAATTGCGGGTGACCCAGGCGGGTCCG
CGCGACCGCGCGCTGGTGATGTTCCTGATCGAATCGCTCGACGACGACGGTTATCTCGGCGCGGGGCTCGACGAGGTGTT
GACCGACCTGCCCGAGGAACTGGAGGTCGATCTCGACGAACTGGGCGCCGCGCTGGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTGGGGGCGCGCTCCGCATCAGAATGCTTGCGCCTGCAATTGTTGCGACTCGATCCGTCCCCCACGCGCACGCTG
TCGCTCGACATCGTCTCGCAGCATCTCGAATTGCTGGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAGTTGAAGGCCAA
CGACGACGAGCTGCGCGAGGCCCATGCACTGATCCGCTCGCTCGAGCCCTTCCCGGGCGCCGCCTACGGCAAGACCGAGG
CGGACTACGTGGTGCCCGACATCATGGTCAGGAAGAGCGGCCAGAACTGGCTCGCGGAGCTCAATCCCGAGGTGGTGCCG
AAGCTGCGCATCAACCACCTGTACGCCAATATCCTGCGCAACAGCCGGGGCGATCCGGGCGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGAGACCATCCTGCGTGTCGCGCAGGCCATCGTGG
AGCGTCAGAAGAACTTTTTTGCGCATGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAAGTACTT
CTTCGGATCGCACGTGTCGACGGATACCGGGGGCGCTGCCTCTTCCACGGCAATCCGCGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCAGAAATCTCCTCTTTCGGACAGCCGAATAGCCGAGCTGCTGGCGGAACAAGGCTTCGTGGTCGCGCGC
CGCACCGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCCGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A560VPW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691

Multiple sequence alignment