Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LF543_RS05525 Genome accession   NZ_CP045562
Coordinates   1062258..1063640 (-) Length   460 a.a.
NCBI ID   WP_010021765.1    Uniprot ID   A0AAE6P112
Organism   Fructilactobacillus fructivorans strain LF543     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1057258..1068640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LF543_RS05490 (LF543_05445) nusG 1057751..1058311 (-) 561 WP_010021773.1 transcription termination/antitermination protein NusG -
  LF543_RS05495 (LF543_05450) secE 1058414..1058593 (-) 180 WP_010021772.1 preprotein translocase subunit SecE -
  LF543_RS05500 (LF543_05455) rpmG 1058607..1058756 (-) 150 WP_010021771.1 50S ribosomal protein L33 -
  LF543_RS05505 (LF543_05460) - 1058805..1059386 (-) 582 WP_010021770.1 sigma-70 family RNA polymerase sigma factor -
  LF543_RS05510 (LF543_05465) rlmB 1059576..1060328 (-) 753 WP_010021769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LF543_RS05515 (LF543_05470) - 1060318..1060743 (-) 426 WP_010021766.1 Mini-ribonuclease 3 -
  LF543_RS05520 (LF543_05475) cysS 1060749..1062158 (-) 1410 WP_056936290.1 cysteine--tRNA ligase -
  LF543_RS05525 (LF543_05480) radA 1062258..1063640 (-) 1383 WP_010021765.1 DNA repair protein RadA Machinery gene
  LF543_RS05530 (LF543_05485) - 1063656..1064192 (-) 537 WP_010021764.1 hypothetical protein -
  LF543_RS05535 (LF543_05490) rpiA 1064351..1065040 (+) 690 WP_010021762.1 ribose-5-phosphate isomerase RpiA -
  LF543_RS05540 (LF543_05495) - 1065136..1065807 (-) 672 WP_010021761.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
  LF543_RS05545 (LF543_05500) - 1065831..1066696 (-) 866 Protein_1058 DMT family transporter -
  LF543_RS05550 (LF543_05505) - 1066856..1067803 (-) 948 WP_010021757.1 AEC family transporter -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49998.48 Da        Isoelectric Point: 8.7704

>NTDB_id=395175 LF543_RS05525 WP_010021765.1 1062258..1063640(-) (radA) [Fructilactobacillus fructivorans strain LF543]
MAKTKTQYVCSNCGYISPRYMGRCPNCGEWNTMVEQTVQKTDPKKANVSRVSLSGNKSRPQLIGQVNAESAPRVQVDSNE
LNRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLAESGQRVLYVSGEESADQIKMRADRLNVSENGNLYIYPETDMAS
IRKAIEEVKPDTVIIDSVQTMQEDEIPSAIGSVTQVRGVTSDLMNIAKTNGITIFLVGHVTKGGTLAGPKTLEHMVDTVL
YFEGDKHHSYRLLRAVKNRFGSTNELGIFQMDEDGLKEVKNPSEIFLEERLKNATGSAIVVAMEGTRPILVELQALIVPS
VFGNAQRTASGLDRNRVSLIMAVLEKRAGLMLQNQDAYLKAAGGVKLNEPAIDLAMAISIASSYENVGTDPKECYIGELG
LTGEIRRVNRIEQRVIEAQKLGFKRVLVPKYNLKGWTPPKNIQVVGVSTIGEALKTALKK

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=395175 LF543_RS05525 WP_010021765.1 1062258..1063640(-) (radA) [Fructilactobacillus fructivorans strain LF543]
ATGGCTAAAACAAAAACACAATACGTTTGTTCCAACTGTGGATACATTTCACCACGTTATATGGGACGTTGTCCCAACTG
TGGCGAGTGGAACACGATGGTGGAGCAGACCGTTCAGAAGACCGATCCGAAAAAAGCTAATGTTAGTCGGGTTTCCCTTA
GTGGCAACAAATCACGACCGCAGTTAATCGGACAGGTTAACGCAGAAAGTGCTCCTAGGGTCCAAGTAGACTCAAATGAA
TTAAATCGTGTCCTAGGTGGTGGAATCGTACCCGGGTCACTGGTATTAATTGGTGGTGATCCAGGAATTGGTAAGTCGAC
CTTACTCTTGCAAGTTTCTGGTCAGTTGGCTGAATCAGGGCAAAGGGTCCTCTATGTTTCGGGGGAAGAAAGTGCCGATC
AGATTAAAATGAGGGCTGATCGACTGAATGTTTCCGAAAATGGTAATTTATACATTTATCCTGAGACTGACATGGCTTCA
ATCAGAAAAGCCATTGAAGAAGTAAAACCCGATACGGTCATTATTGATTCAGTTCAGACCATGCAGGAGGATGAAATTCC
CTCTGCCATTGGATCTGTGACCCAAGTTCGTGGAGTTACCTCTGACTTAATGAATATTGCTAAAACGAATGGAATTACTA
TTTTCTTAGTGGGTCATGTTACCAAAGGTGGAACGCTTGCAGGCCCTAAGACACTTGAACACATGGTGGATACCGTCCTT
TATTTTGAAGGAGACAAACATCACTCTTATCGACTATTACGGGCCGTTAAGAATCGATTTGGTTCTACTAACGAGCTAGG
AATCTTTCAGATGGACGAAGACGGTCTCAAAGAGGTTAAAAATCCATCCGAAATTTTTCTGGAAGAACGCTTAAAGAATG
CCACCGGCTCTGCCATTGTGGTAGCAATGGAGGGAACTCGTCCGATTCTAGTGGAATTACAGGCTCTGATTGTTCCATCG
GTTTTTGGGAACGCACAAAGGACCGCTTCTGGTCTTGATCGGAACCGGGTCTCGTTGATTATGGCGGTTTTAGAAAAACG
AGCTGGATTAATGTTGCAAAATCAGGACGCCTATTTAAAGGCTGCTGGTGGAGTGAAGTTAAACGAACCAGCAATTGACC
TGGCAATGGCAATCAGTATTGCTTCCAGTTACGAAAACGTGGGAACCGATCCGAAAGAATGCTACATTGGTGAACTTGGC
TTGACTGGTGAAATTCGACGGGTTAATCGAATTGAACAACGCGTCATTGAGGCCCAAAAGTTAGGCTTTAAGCGAGTACT
TGTTCCCAAATATAACTTAAAGGGATGGACGCCACCAAAGAACATTCAAGTGGTGGGCGTCTCGACCATTGGTGAAGCAT
TGAAGACGGCACTAAAAAAGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(79-224)

Subunit ChlI of Mg-chelatase (ChlI)

(365-432)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

65.055

98.913

0.643
  radA Streptococcus mitis SK321

64.835

98.913

0.641
  radA Streptococcus pneumoniae Rx1

64.835

98.913

0.641
  radA Streptococcus pneumoniae D39

64.835

98.913

0.641
  radA Streptococcus pneumoniae R6

64.835

98.913

0.641
  radA Streptococcus pneumoniae TIGR4

64.835

98.913

0.641
  radA Bacillus subtilis subsp. subtilis str. 168

62.009

99.565

0.617

Multiple sequence alignment