Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GD578_RS02490 Genome accession   NZ_CP045560
Coordinates   531420..532196 (+) Length   258 a.a.
NCBI ID   WP_308646142.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain AC1530     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 526420..537196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GD578_RS02465 (GD578_02485) - 527306..528604 (+) 1299 WP_081401011.1 glycosyltransferase family 1 protein -
  GD578_RS02470 (GD578_02490) - 528629..529180 (+) 552 WP_002053282.1 phosphatase PAP2 family protein -
  GD578_RS02475 (GD578_02495) iscX 529280..529480 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  GD578_RS02480 (GD578_02500) ndk 529594..530025 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  GD578_RS02485 (GD578_02505) rlmN 530148..531380 (+) 1233 WP_002053401.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GD578_RS02490 (GD578_02510) pilF 531420..532196 (+) 777 WP_308646142.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GD578_RS02495 (GD578_02515) - 532187..532978 (+) 792 WP_002053491.1 RodZ domain-containing protein -
  GD578_RS02500 (GD578_02520) ispG 533000..534115 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GD578_RS02505 (GD578_02525) hisS 534112..535404 (+) 1293 WP_002053284.1 histidine--tRNA ligase -
  GD578_RS02510 (GD578_02530) - 535433..536131 (+) 699 WP_002053293.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 258 a.a.        Molecular weight: 29154.25 Da        Isoelectric Point: 8.5456

>NTDB_id=395131 GD578_RS02490 WP_308646142.1 531420..532196(+) (pilF) [Acinetobacter nosocomialis strain AC1530]
MLCMGVAVACLASGCQSSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGILLQQEGSKP
NLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDIASAEKTFK
QALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQLRALFPES
PEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 777 bp        

>NTDB_id=395131 GD578_RS02490 WP_308646142.1 531420..532196(+) (pilF) [Acinetobacter nosocomialis strain AC1530]
ATGTTATGTATGGGGGTTGCTGTTGCATGTCTGGCCAGTGGTTGTCAGTCTTCGCCAACACTTAAAAAGGACCCGGAAAA
AGCAGTTAAGGTGCGTACGCAGCTTGCCGCTGAATACATTCGTTCTGGTGACTTGGACTCTGCAAAGCGTTCCCTTGACC
AAGCTTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTATTGCAACAAGAAGGCAGTAAACCC
AATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCATCTGAGCCAGATAATGCTCAGGCGCGTAATAATTATGG
TACGTATTTGTACCAAATGGAGCGTTATAATGATGCCATTGAGCAATTCCGTATTGCTGGTGCAACATTAGGTTATGATC
AACGCTATCAAGCCTTAGAAAATCTAGGGCGGATTTATTTAAAATTAGGTGATATTGCGAGTGCTGAAAAAACATTCAAA
CAAGCACTCCTTGCTAACCGCGATTCTTACATTTCAATGTTAGAGTTAGCAGAAATTTTTTATTTGCAGCAGCAGATTCC
GGCTGCTACACAAATGTATGAACAATACGTTCGTACAGTGGGGCAGAAAAATCAGGGTGCTCGCGCACTTTGGATTGGTT
TAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAGTTACGTGCTTTATTCCCGGAAAGT
CCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(141-198)

Tetratricopeptide repeat (TPR_16)

(81-126)

Tetratricopeptide repeat (TPR_8)

(31-60)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

98.45

100

0.985
  pilF Acinetobacter baylyi ADP1

75

99.225

0.744

Multiple sequence alignment