Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   SPSINT_RS01075 Genome accession   NC_014925
Coordinates   232259..233629 (+) Length   456 a.a.
NCBI ID   WP_015728606.1    Uniprot ID   -
Organism   Staphylococcus pseudintermedius HKU10-03     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 227259..238629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPSINT_RS01050 (SPSINT_0193) - 227403..228623 (+) 1221 WP_014614774.1 YcaO-like family protein -
  SPSINT_RS01055 (SPSINT_0194) - 228643..229455 (+) 813 WP_015728603.1 SagB family peptide dehydrogenase -
  SPSINT_RS01060 (SPSINT_0195) - 229455..230414 (+) 960 WP_233511255.1 hypothetical protein -
  SPSINT_RS01065 (SPSINT_0196) - 230429..231133 (+) 705 WP_014614771.1 ABC transporter ATP-binding protein -
  SPSINT_RS01070 (SPSINT_0197) - 231120..231866 (+) 747 WP_015728605.1 ABC transporter permease -
  SPSINT_RS01075 (SPSINT_0198) radA 232259..233629 (+) 1371 WP_015728606.1 DNA repair protein RadA Machinery gene
  SPSINT_RS01080 (SPSINT_0199) - 233648..234709 (+) 1062 WP_014614768.1 PIN/TRAM domain-containing protein -
  SPSINT_RS01085 (SPSINT_0200) ispD 234710..235408 (+) 699 WP_014614767.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  SPSINT_RS01090 (SPSINT_0201) ispF 235401..235880 (+) 480 WP_014614766.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  SPSINT_RS01095 (SPSINT_0202) gltX 235957..237411 (+) 1455 WP_015728607.1 glutamate--tRNA ligase -
  SPSINT_RS01100 (SPSINT_0203) cysE 237799..238464 (+) 666 WP_172425792.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50028.50 Da        Isoelectric Point: 7.0051

>NTDB_id=39500 SPSINT_RS01075 WP_015728606.1 232259..233629(+) (radA) [Staphylococcus pseudintermedius HKU10-03]
MAKTKTIFECTACGYQSPKWMGKCPNCGAWNSMEESFEQKTASPKHGVRAQKTSSAKIQKLDDIKQELAPRIQTNSGELN
RVLGGGIVEGSLVLIGGDPGIGKSTLLLQMCAALSKEKRVLYITGEESLNQTKIRADRLDEDASQLNVFAETDLMVIHEA
VKKVEPDLIVVDSIQTIYHPEISSAPGSVSQVRESTQSLMHIAKQMNVATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEG
DEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKSVLNPSEMFLEERTTNVAGSTIVATMEGTRPLLIEVQALVTPTTFNN
PRRMATGIDHNRLSLLMAVLEKKEGYLLQQQDAYIKVAGGVKLSEPAVDLGIVIATASSFKDQAVDGFDCYIGEVGLTGE
VRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWHFPEGIEVIGVTSVNEALKYALKN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=39500 SPSINT_RS01075 WP_015728606.1 232259..233629(+) (radA) [Staphylococcus pseudintermedius HKU10-03]
GTGGCAAAAACGAAAACAATTTTTGAATGTACGGCCTGTGGTTATCAATCGCCTAAATGGATGGGGAAATGTCCAAATTG
TGGTGCTTGGAATTCAATGGAGGAATCTTTTGAACAAAAAACTGCGAGTCCTAAACACGGTGTACGGGCGCAAAAAACAA
GTTCAGCAAAAATACAAAAGCTCGATGACATTAAACAAGAGCTTGCGCCACGTATCCAAACGAATAGTGGTGAATTAAAC
CGTGTGCTCGGTGGCGGTATTGTAGAAGGGTCACTCGTGCTGATTGGTGGCGATCCAGGAATAGGGAAGTCCACATTGTT
ACTTCAAATGTGCGCGGCTTTATCTAAAGAGAAAAGGGTGCTCTATATTACTGGTGAGGAATCACTCAATCAAACTAAAA
TTAGAGCGGATCGTTTGGATGAAGATGCGAGTCAACTCAACGTGTTTGCGGAAACGGACTTAATGGTTATTCATGAAGCG
GTGAAAAAGGTCGAACCTGATTTAATTGTAGTGGACTCTATTCAGACGATCTACCATCCCGAAATCAGTTCGGCGCCTGG
CTCTGTATCTCAAGTTCGTGAAAGTACACAAAGCTTGATGCACATCGCTAAACAAATGAATGTTGCGACGTTTATTGTAG
GCCATGTGACGAAAGAAGGCCAAATCGCGGGGCCGCGTTTACTTGAACATATGGTGGATACGGTGCTTTATTTTGAAGGT
GATGAACATCATGCGTATCGTATTTTAAGAGCGGTGAAAAACCGTTTTGGCTCTACGAATGAAATGGGTATTTTTGAAAT
GAAACAGTCAGGTTTAAAAAGCGTGCTTAACCCGTCAGAAATGTTTTTAGAAGAACGGACGACCAATGTTGCAGGTTCGA
CAATTGTCGCAACGATGGAAGGGACACGACCATTGTTAATTGAAGTACAAGCGCTTGTCACACCGACGACGTTCAATAAT
CCGAGACGTATGGCGACAGGTATTGACCATAATCGCCTCAGTTTGTTAATGGCGGTGTTAGAGAAAAAAGAAGGTTATTT
ATTACAACAACAAGATGCTTACATTAAGGTGGCAGGGGGCGTCAAACTCTCTGAACCTGCTGTCGACTTAGGTATCGTCA
TTGCGACAGCTTCTAGTTTTAAAGACCAAGCAGTAGACGGCTTCGATTGTTATATTGGCGAGGTTGGATTGACTGGTGAG
GTCCGCCGTGTTTCGCGCATTGAGCAACGTGTGCAAGAAGCAGCAAAATTGGGCTTTAAACGTGTGATTATCCCTAAAAA
CAATATTGGCGGATGGCACTTTCCAGAAGGTATTGAAGTGATTGGCGTGACTTCTGTCAATGAAGCATTGAAATACGCAT
TAAAAAATTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-220)

Subunit ChlI of Mg-chelatase (ChlI)

(345-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.789

100

0.658
  radA Streptococcus pneumoniae Rx1

58.628

99.123

0.581
  radA Streptococcus pneumoniae D39

58.628

99.123

0.581
  radA Streptococcus pneumoniae R6

58.628

99.123

0.581
  radA Streptococcus pneumoniae TIGR4

58.628

99.123

0.581
  radA Streptococcus mitis NCTC 12261

58.628

99.123

0.581
  radA Streptococcus mitis SK321

58.628

99.123

0.581

Multiple sequence alignment