Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HPY14_RS11865 Genome accession   NZ_CP053798
Coordinates   2619505..2621232 (-) Length   575 a.a.
NCBI ID   WP_094392768.1    Uniprot ID   -
Organism   Vibrio cholerae strain SL5Y     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2614505..2626232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY14_RS11830 (HPY14_11830) - 2614807..2615577 (-) 771 WP_042989134.1 PilW family protein -
  HPY14_RS11835 (HPY14_11835) - 2615577..2616146 (-) 570 WP_162298898.1 type II secretion system protein -
  HPY14_RS11840 (HPY14_11840) - 2616136..2616648 (-) 513 WP_001249938.1 type II secretion system protein -
  HPY14_RS11845 (HPY14_11845) - 2616711..2617148 (-) 438 WP_094392766.1 type II secretion system protein -
  HPY14_RS11850 (HPY14_11850) - 2617183..2617737 (-) 555 WP_000738708.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HPY14_RS11855 (HPY14_11855) - 2617818..2618270 (-) 453 WP_094392767.1 MSHA biogenesis protein MshF -
  HPY14_RS11860 (HPY14_11860) - 2618273..2619496 (-) 1224 WP_000190444.1 type II secretion system F family protein -
  HPY14_RS11865 (HPY14_11865) pilB/pilB1 2619505..2621232 (-) 1728 WP_094392768.1 GspE/PulE family protein Machinery gene
  HPY14_RS11870 (HPY14_11870) - 2621222..2622373 (-) 1152 WP_162298899.1 lipopolysaccharide assembly protein LapB -
  HPY14_RS11875 (HPY14_11875) - 2622370..2623215 (-) 846 WP_000281360.1 ExeA family protein -
  HPY14_RS11880 (HPY14_11880) mshL 2623220..2624899 (-) 1680 WP_094392770.1 pilus (MSHA type) biogenesis protein MshL -
  HPY14_RS11885 (HPY14_11885) - 2624914..2625231 (-) 318 WP_000252812.1 MSHA biogenesis protein MshK -
  HPY14_RS11890 (HPY14_11890) gspM 2625224..2625874 (-) 651 WP_094392771.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63953.46 Da        Isoelectric Point: 6.1332

>NTDB_id=394915 HPY14_RS11865 WP_094392768.1 2619505..2621232(-) (pilB/pilB1) [Vibrio cholerae strain SL5Y]
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPEYVFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSEEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRVNQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDHETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGSPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
VAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVEPIYL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=394915 HPY14_RS11865 WP_094392768.1 2619505..2621232(-) (pilB/pilB1) [Vibrio cholerae strain SL5Y]
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGTTGGTTGAAGAGGGGATTGTGAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTACCTGTGATCGATTTGAGCCGCGCTCATGTTGATATTGATGCGGTTCCT
TTACTGCCTGAAGTCCATGCCCGCCGTTTGCGTGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATCTGTTTGCTCAAGAAGCCTTGCTGAACCAGTTGCCTGAATACGTCTTTGAGTTTGTCATCGCCCCTG
AAAAGCAGCTGGTGGATGGCTTTGATCGTTACTATCGCCGCACCAAAGAGATTGTCTCATTTGCCGAGCAATTGCATGCT
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACTGATTCAGATAGCGAAGAAGTGACGGTTGTTAAACTGATCAA
CTCGTTGTTTGAAGATGCGATTCAAGTGGGAGCCTCGGATATTCATATCGAGCCGGATGCCAACGTACTGCGTTTGCGGC
AACGGATCGATGGGGTATTACATGAAACCTTGCTCCATGAAGTGAATATCGCCTCTGCCTTGGTACTGCGCTTAAAATTG
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGCCGCTTTAACATCCGTGTAAAAGGACAATCAGTCGA
TATCCGGATGTCAACCATGCCTATCCAGCATGGTGAATCAGTGGTGATGCGTCTGCTTAACCAATCTGCTGGAGTTCGAA
AGCTCGAAGAGTCCGGTATTCCACCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCCCATGGCATGATTCTG
GTGACTGGACCTACCGGCTCAGGGAAAACCACCACCTTGTATGGCGCACTCAACGAGCTCAATACGTCCGGTAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATATCGAATTTCCAGGGTTAATCAGGTGCAAGTTAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACCTTCTTGCGCCAAGACCCAGATATTATTCTGGTCGGTGAGATGCGGGACCACGAGACAGTC
GAAATTGGGCTGCGCGCCGCATTAACCGGTCACTTAGTGTTAAGTACTTTGCATACCAATGATGCCGTCGATAGTGCGCT
GCGTTTGATTGATATGGGCTCGCCCGGTTATCTGGTTGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTACGAA
AAGTCTGTCCGGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAA
GTTGCCGCCAAATTCACTCGTGGACGCGGCTGTCAAAACTGTAACTTAACTGGTTATCGCGGCCGGATCGGTGTATTTGA
AATGCTTGAGCTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCTGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTTGGCGCTGCAAGGTATCGTCAGTCTTGATGAAGTGATGTCG
CTGGGTGAAGGTGACTCCTCTGGTTTGGTTGAACCGATTTATCTGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(189-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.265

100

0.37
  pilB Vibrio cholerae strain A1552

37.193

99.13

0.369