Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LP475_RS01670 Genome accession   NZ_CP045530
Coordinates   365164..366540 (+) Length   458 a.a.
NCBI ID   WP_057807500.1    Uniprot ID   -
Organism   Limosilactobacillus pontis strain LP475     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 360164..371540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP475_RS01640 (LP475_01645) - 360680..361543 (+) 864 WP_057807516.1 alpha/beta hydrolase -
  LP475_RS01645 (LP475_01650) - 361603..362943 (-) 1341 WP_057807514.1 aminopeptidase C -
  LP475_RS01650 - 363087..363242 (+) 156 WP_152936711.1 hypothetical protein -
  LP475_RS01655 (LP475_01655) rpiA 363490..364173 (-) 684 WP_057807504.1 ribose-5-phosphate isomerase RpiA -
  LP475_RS01660 (LP475_01660) - 364192..364500 (-) 309 WP_420814142.1 GNAT family N-acetyltransferase -
  LP475_RS01665 (LP475_01665) - 364614..365150 (+) 537 WP_057807502.1 hypothetical protein -
  LP475_RS01670 (LP475_01670) radA 365164..366540 (+) 1377 WP_057807500.1 DNA repair protein RadA Machinery gene
  LP475_RS01675 (LP475_01675) - 366564..367724 (+) 1161 WP_057807498.1 PIN/TRAM domain-containing protein -
  LP475_RS01680 (LP475_01680) cysS 368098..369510 (+) 1413 WP_057807496.1 cysteine--tRNA ligase -
  LP475_RS01685 (LP475_01685) - 369523..369936 (+) 414 WP_057807494.1 Mini-ribonuclease 3 -
  LP475_RS01690 (LP475_01690) rlmB 369926..370675 (+) 750 WP_152936712.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50115.39 Da        Isoelectric Point: 8.9730

>NTDB_id=394913 LP475_RS01670 WP_057807500.1 365164..366540(+) (radA) [Limosilactobacillus pontis strain LP475]
MAKLRTQYVCQTCGYNSPRYLGRCPNCGEWNTFVEEQVQSSTTTATKAKTTTLTGLVSKPQKISEINSQETPRVRTRLNE
LNRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSDDHHRVLYVSGEESGTQIKMRAERLHVAGDNFYVYPETNMDSI
RDTIRELKPEFVVIDSVQTMQATDVSSAIGSVSQIREVTAQLMQIAKSNNITIFVVGHVTKGGALAGPKILEHMVDTVLY
FEGDLHHTYRILRSVKNRFGSTNELGIFEMHTNGLTEVKNPSEIFLEERLRDATGSAVVVSLEGTRPILVEIQALVTPTV
YGNAQRTATGLNRNRVSLIMAVLEKRASLMLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTQASDAFVGEVGL
TGEIRRVNRIEQRVAEAQKLGFRRIFVPKNNLKGWTPPTGIEVIGVATLREALRLVLA

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=394913 LP475_RS01670 WP_057807500.1 365164..366540(+) (radA) [Limosilactobacillus pontis strain LP475]
ATGGCAAAGCTAAGAACACAGTACGTCTGCCAGACCTGCGGTTATAATTCGCCCCGCTACCTGGGGCGGTGCCCAAACTG
TGGGGAGTGGAACACCTTCGTCGAGGAGCAGGTGCAAAGCAGCACTACGACGGCCACCAAGGCCAAGACAACCACACTGA
CCGGGCTGGTGTCTAAACCGCAGAAAATCAGTGAGATCAACAGTCAGGAGACACCCCGGGTCAGGACACGACTCAACGAG
CTCAACCGGGTCCTTGGCGGCGGAATCGTGCCCGGCTCCCTGATCCTGATCGGTGGGGACCCCGGTATTGGGAAGTCAAC
CCTCCTCCTCCAGGTTTCGGGCCAGCTGAGTGATGACCACCATCGAGTTCTCTACGTTTCTGGGGAGGAGAGCGGCACCC
AGATTAAGATGCGGGCGGAACGGCTTCATGTGGCAGGAGACAACTTCTACGTCTACCCGGAGACCAACATGGATAGTATT
CGCGACACAATCCGTGAGCTTAAGCCGGAGTTCGTGGTGATTGACTCCGTGCAGACGATGCAGGCGACCGACGTCAGTTC
TGCGATCGGTAGTGTTTCCCAGATTCGGGAAGTGACCGCCCAGCTGATGCAGATTGCCAAGAGCAACAACATCACGATTT
TCGTGGTCGGCCACGTCACCAAGGGCGGGGCACTGGCCGGGCCGAAGATCTTGGAGCACATGGTGGATACCGTCCTCTAC
TTTGAGGGGGACCTCCACCACACCTACCGGATCCTGCGTTCGGTGAAGAACCGGTTCGGGTCGACCAACGAGCTGGGAAT
CTTTGAGATGCACACCAACGGGTTGACCGAGGTCAAGAACCCGTCCGAGATCTTCTTGGAGGAGCGGCTCCGTGACGCTA
CCGGGTCGGCAGTCGTGGTTTCTTTGGAGGGAACCCGCCCGATTTTAGTTGAGATTCAGGCCCTAGTTACGCCGACGGTC
TACGGGAATGCCCAGCGAACGGCTACCGGTTTGAACCGGAATCGGGTATCATTGATTATGGCGGTACTGGAGAAGCGAGC
CAGCCTGATGTTGCAAAACCAGGATGCCTATCTGAAGGCAGCCGGTGGGGTCAAGCTGGATGAGCCAGCGATTGACCTGG
CCATTGCGGTGGCGATCGCGTCGAGTTACCGTGACAAGGGGACCCAGGCGAGTGACGCCTTCGTCGGTGAGGTTGGATTG
ACCGGGGAAATTCGCCGGGTCAACCGGATCGAACAGCGAGTGGCAGAGGCGCAGAAGCTGGGCTTCCGGCGGATCTTCGT
ACCGAAGAATAACCTGAAGGGCTGGACACCCCCGACCGGGATCGAGGTTATTGGGGTCGCTACCCTGCGTGAGGCCCTGC
GTCTCGTCCTGGCATGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(64-223)

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.376

100

0.664
  radA Streptococcus mitis NCTC 12261

66.376

100

0.664
  radA Streptococcus pneumoniae Rx1

66.157

100

0.662
  radA Streptococcus pneumoniae D39

66.157

100

0.662
  radA Streptococcus pneumoniae R6

66.157

100

0.662
  radA Streptococcus pneumoniae TIGR4

66.157

100

0.662
  radA Bacillus subtilis subsp. subtilis str. 168

63.02

99.782

0.629

Multiple sequence alignment