Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HQN81_RS03075 Genome accession   NZ_CP053787
Coordinates   645275..645715 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli isolate J31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 640275..650715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQN81_RS03055 (HQN81_03055) coaE 640765..641385 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  HQN81_RS24095 - 641410..641454 (+) 45 WP_120795372.1 protein YacM -
  HQN81_RS03060 (HQN81_03060) guaC 641610..642653 (+) 1044 WP_001217338.1 GMP reductase -
  HQN81_RS03065 (HQN81_03065) hofC 642688..643890 (-) 1203 WP_000157266.1 protein transport protein HofC -
  HQN81_RS03070 (HQN81_03070) pilB 643880..645265 (-) 1386 WP_172614059.1 type II secretion system protein GspE Machinery gene
  HQN81_RS03075 (HQN81_03075) pilA 645275..645715 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  HQN81_RS03080 (HQN81_03080) nadC 645918..646811 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HQN81_RS03085 (HQN81_03085) ampD 646899..647450 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HQN81_RS03090 (HQN81_03090) ampE 647447..648301 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  HQN81_RS03095 (HQN81_03095) aroP 648344..649717 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=394294 HQN81_RS03075 WP_000360904.1 645275..645715(-) (pilA) [Escherichia coli isolate J31]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=394294 HQN81_RS03075 WP_000360904.1 645275..645715(-) (pilA) [Escherichia coli isolate J31]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363