Detailed information NTG0394021

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: GCU76_RS20770
• Genome accession: NZ_CP045425
• Coordinates: 3832788..3833933 (+)
• Length: 381 a.a.
• NCBI ID: WP_017696253.1
• Uniprot ID: A0A0C3JF17
• Organism: Bacillus subtilis strain JAAA
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3827788..3838933

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCU76_RS20745	albF	3827803..3829083 (+)	1281	WP_017696258.1	pitrilysin family protein	-
GCU76_RS20750	albG	3829080..3829781 (+)	702	WP_017696257.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
GCU76_RS20755	ywhL	3829787..3831154 (-)	1368	WP_017696256.1	YncE family protein	-
GCU76_RS20760	ywhK	3831203..3832558 (-)	1356	WP_017696255.1	YncE family protein	-
GCU76_RS23320	-	3832555..3832785 (-)	231	WP_041334639.1	hypothetical protein	-
GCU76_RS20770	rapF	3832788..3833933 (+)	1146	WP_017696253.1	tetratricopeptide repeat protein	Regulator
GCU76_RS20775	-	3833917..3834036 (+)	120	WP_014665774.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
GCU76_RS20780	ywhH	3834135..3834600 (+)	466	Protein_4009	YbaK/EbsC family protein	-
GCU76_RS20785	speB	3834632..3835504 (-)	873	WP_003227545.1	agmatinase	-
GCU76_RS20790	speE	3835565..3836395 (-)	831	WP_003227543.1	spermidine synthase	-
GCU76_RS20795	pbpG	3836598..3838673 (+)	2076	WP_017696252.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45593.24 Da
• Isoelectric Point: 5.0684

>NTDB_id=394021 GCU76_RS20770 WP_017696253.1 3832788..3833933(+) (rapF) [Bacillus subtilis strain JAAA]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDRREYVLAIQFFKKAESKLVFVKDKIDKAEFFFKMSEVYYYMKQTYVS
MDYARQAFEIYSEQTTYDIRMIQCQSLFGANFLDLKQYEKAIAHFQKAFTLAKTEQQPQLMGRTLYNIALCKQNQNSFES
AIEYVKKSIQVFEGSKMLPSLPQAFFLITQIYFKLGKIDNACEYHKKGKEYSAAAGDTVYLTEFEFLESLYLSGPDEERI
EKCFVFLESKMMYADIEDFALDVAKYYHQQENYKKASHYFLKVEEARQHIQGGVNLYEMEV

Nucleotide

• Length: 1146 bp

>NTDB_id=394021 GCU76_RS20770 WP_017696253.1 3832788..3833933(+) (rapF) [Bacillus subtilis strain JAAA]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGACCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGATCGCCGTGAATATGTATTAGCCATTCAATTCTTTAAAAAAGCTGAGAGCAAGCTGGTTTTTG
TCAAAGATAAAATCGATAAAGCTGAATTCTTTTTCAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTCTCC
ATGGACTATGCACGTCAAGCATTCGAAATATACAGTGAACAGACTACATATGATATCAGAATGATTCAATGCCAGTCCTT
ATTCGGAGCCAACTTTTTGGATTTAAAACAATACGAAAAAGCAATTGCCCATTTTCAAAAAGCCTTTACACTTGCAAAAA
CAGAGCAGCAGCCCCAATTAATGGGAAGAACACTTTATAACATAGCACTCTGCAAGCAAAACCAAAACAGTTTTGAATCA
GCGATAGAGTATGTAAAAAAATCTATTCAAGTATTTGAAGGATCTAAAATGCTTCCTTCATTGCCACAAGCTTTTTTCCT
AATTACACAAATTTATTTTAAATTAGGTAAGATTGATAACGCCTGTGAATATCATAAAAAAGGAAAAGAGTATTCTGCAG
CTGCGGGAGATACCGTGTATCTAACGGAGTTTGAATTTTTGGAATCTTTATACTTGTCTGGACCGGATGAGGAACGGATA
GAAAAATGTTTTGTTTTTCTTGAAAGTAAAATGATGTATGCAGATATTGAGGACTTCGCACTTGATGTTGCTAAATATTA
TCATCAACAGGAAAATTACAAAAAAGCATCTCATTACTTTTTAAAGGTTGAAGAAGCAAGACAGCACATTCAAGGGGGGG
TGAATTTATATGAAATGGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_8) (263-290)

• Tetratricopeptide repeat (TPR_12) (189-253)

3D structure

• AlphaFold DB: A0A0C3JF17

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	80.052	100	0.801
rapC	Bacillus subtilis subsp. subtilis str. 168	57.105	99.738	0.57