Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FIU95_RS19870 Genome accession   NZ_CP045369
Coordinates   4567818..4568825 (-) Length   335 a.a.
NCBI ID   WP_216646373.1    Uniprot ID   -
Organism   Microbulbifer sp. THAF38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4562818..4573825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU95_RS19845 (FIU95_20545) trmB 4563669..4564454 (-) 786 WP_152455831.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  FIU95_RS19850 (FIU95_20550) - 4564517..4564894 (-) 378 WP_152455833.1 DUF423 domain-containing protein -
  FIU95_RS19855 (FIU95_20555) rpoH 4565001..4565855 (-) 855 WP_152455835.1 RNA polymerase sigma factor RpoH -
  FIU95_RS19860 (FIU95_20560) ftsX 4566094..4567110 (-) 1017 WP_152455837.1 permease-like cell division protein FtsX -
  FIU95_RS19865 (FIU95_20565) ftsE 4567091..4567777 (-) 687 WP_152455839.1 cell division ATP-binding protein FtsE -
  FIU95_RS19870 (FIU95_20570) pilA 4567818..4568825 (-) 1008 WP_216646373.1 signal recognition particle-docking protein FtsY Machinery gene
  FIU95_RS19875 (FIU95_20575) rsmD 4569265..4569864 (+) 600 WP_152455843.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  FIU95_RS19880 (FIU95_20580) coaD 4569961..4570437 (+) 477 WP_152455845.1 pantetheine-phosphate adenylyltransferase -
  FIU95_RS19885 (FIU95_20585) ggt 4570500..4572218 (+) 1719 WP_152455847.1 gamma-glutamyltransferase -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 36097.28 Da        Isoelectric Point: 5.5021

>NTDB_id=393716 FIU95_RS19870 WP_216646373.1 4567818..4568825(-) (pilA) [Microbulbifer sp. THAF38]
MLEPAPQVQEPAPKPTPVQEKPAEKKKEGFFARIRRGLSRTSNQFVEGMGNLFLGAKEIDEDLLEELETQLLMADVGVEA
TSEIVDRLTERVSRRELADGDALYKALQQELAELLNSVDAPLVLDTSKKPYVILVVGVNGVGKTTTIGKLAHRFLGEGKS
VMLAAGDTFRAAAVEQLQVWGQRHDVPVVAQHTGADSASVIFDAVQSAQARGIDVVIADTAGRLHTKSNLMEELTKVRRV
MGKLDPSAPHEVLLVLDAGTGQNAINQAAQFREAAGVTGLVLTKLDGTAKGGVIFALARRFGIPVRFIGVGEQAEDLQPF
RAREFVSALFGRDTA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=393716 FIU95_RS19870 WP_216646373.1 4567818..4568825(-) (pilA) [Microbulbifer sp. THAF38]
GTGTTGGAACCGGCTCCTCAGGTACAGGAGCCCGCGCCCAAGCCCACTCCTGTGCAGGAAAAACCTGCAGAGAAGAAGAA
AGAGGGTTTCTTTGCGCGTATTCGCCGCGGGCTCAGCCGCACCAGCAATCAGTTTGTCGAGGGGATGGGCAACCTGTTCC
TCGGAGCCAAAGAGATCGATGAAGATCTGTTGGAAGAGCTGGAAACCCAGTTGCTGATGGCGGATGTGGGCGTCGAGGCC
ACCAGTGAAATTGTCGATCGCCTCACCGAGCGCGTTTCACGCCGCGAACTCGCCGATGGCGATGCGCTCTATAAAGCGCT
GCAGCAGGAGTTGGCAGAGCTGCTCAACAGTGTCGATGCGCCCCTGGTGCTGGACACCAGCAAAAAGCCCTATGTAATTC
TGGTGGTCGGCGTTAACGGCGTCGGCAAGACCACCACTATCGGCAAGTTGGCACACCGCTTCCTCGGCGAGGGCAAATCG
GTGATGCTCGCCGCCGGCGATACTTTCCGTGCCGCTGCGGTGGAGCAGTTACAGGTTTGGGGGCAGCGACACGATGTGCC
AGTAGTGGCCCAGCATACCGGTGCGGACAGCGCCTCGGTGATTTTCGATGCCGTGCAGTCGGCCCAGGCTCGAGGTATCG
ATGTGGTGATTGCCGATACAGCCGGCCGCTTGCACACCAAGTCCAACCTGATGGAAGAACTGACCAAGGTGCGCCGCGTA
ATGGGCAAGCTGGACCCCAGCGCCCCCCACGAGGTGCTGTTGGTATTGGATGCCGGCACCGGCCAGAATGCGATTAACCA
AGCCGCACAGTTTCGCGAGGCGGCCGGTGTTACCGGCCTGGTGCTCACCAAGCTCGACGGCACCGCCAAGGGTGGGGTGA
TTTTCGCTCTGGCGCGGCGCTTCGGTATTCCCGTGCGCTTTATCGGTGTGGGTGAACAGGCTGAGGACCTGCAGCCGTTT
CGCGCGCGCGAATTTGTCTCGGCGCTGTTTGGCCGCGATACCGCCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(131-330)

SRP54-type protein, helical bundle domain (SRP54_N)

(47-111)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

54.603

94.03

0.513

Multiple sequence alignment