Detailed information NTG0393597

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HPT09_RS27485
• Genome accession: NZ_CP053706
• Coordinates: 5951271..5952419 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PAC1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5946271..5957419

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HPT09_RS27460 (HPT09_27465)	-	5947422..5947718 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HPT09_RS27465 (HPT09_27470)	-	5947715..5948308 (-)	594	WP_003084544.1	YggT family protein	-
HPT09_RS27470 (HPT09_27475)	proC	5948319..5949140 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HPT09_RS27475 (HPT09_27480)	-	5949152..5949844 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
HPT09_RS27480 (HPT09_27485)	pilT	5950059..5951093 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HPT09_RS27485 (HPT09_27490)	pilU	5951271..5952419 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HPT09_RS27490 (HPT09_27495)	czcD	5952427..5953326 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HPT09_RS27495 (HPT09_27500)	-	5953480..5953884 (+)	405	WP_003084560.1	NINE protein	-
HPT09_RS27500 (HPT09_27505)	-	5954127..5955500 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HPT09_RS27505 (HPT09_27510)	-	5955497..5956681 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=393597 HPT09_RS27485 WP_003100959.1 5951271..5952419(+) (pilU) [Pseudomonas aeruginosa strain PAC1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=393597 HPT09_RS27485 WP_003100959.1 5951271..5952419(+) (pilU) [Pseudomonas aeruginosa strain PAC1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382