Detailed information NTG0393546

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FIV02_RS22180
• Genome accession: NZ_CP045349
• Coordinates: 4752792..4753937 (-)
• Length: 381 a.a.
• NCBI ID: WP_037056536.1
• Uniprot ID: A0A5P9CD92
• Organism: Pseudomonas sp. THAF187a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4747792..4758937

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIV02_RS22160 (FIV02_22075)	ntrC	4748612..4750045 (+)	1434	WP_037056525.1	nitrogen regulation protein NR(I)	-
FIV02_RS22165 (FIV02_22080)	-	4750185..4750583 (-)	399	WP_037056528.1	TM2 domain-containing protein	-
FIV02_RS22170 (FIV02_22085)	-	4750731..4751942 (+)	1212	WP_037056531.1	putative DNA modification/repair radical SAM protein	-
FIV02_RS22175 (FIV02_22090)	-	4751942..4752826 (+)	885	WP_152489805.1	TIGR03915 family putative DNA repair protein	-
FIV02_RS22180 (FIV02_22095)	pilU	4752792..4753937 (-)	1146	WP_037056536.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FIV02_RS22185 (FIV02_22100)	pilT	4754019..4755053 (-)	1035	WP_037056538.1	type IV pilus twitching motility protein PilT	Machinery gene
FIV02_RS22190 (FIV02_22105)	-	4755269..4755961 (+)	693	WP_134676426.1	YggS family pyridoxal phosphate-dependent enzyme	-
FIV02_RS22195 (FIV02_22110)	proC	4755979..4756797 (+)	819	WP_152489806.1	pyrroline-5-carboxylate reductase	-
FIV02_RS22200 (FIV02_22115)	-	4756807..4757400 (+)	594	WP_037056544.1	YggT family protein	-
FIV02_RS22205 (FIV02_22120)	-	4757405..4757695 (+)	291	WP_037056545.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42515.83 Da
• Isoelectric Point: 6.5937

>NTDB_id=393546 FIV02_RS22180 WP_037056536.1 4752792..4753937(-) (pilU) [Pseudomonas sp. THAF187a]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNPMSPEQTRETVLGVMNEAQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYNLYTQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMAKGLELEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=393546 FIV02_RS22180 WP_037056536.1 4752792..4753937(-) (pilU) [Pseudomonas sp. THAF187a]
ATGGAATTCGAAAAACTGCTGCGCCTGATGGTCGAAAAAGGTGGTTCCGACCTGTTCATCACTGCCGGTGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCCGTGACCAAGAACCCCATGTCGCCGGAGCAGACTCGCGAAACCGTGCTCGGCG
TGATGAACGAGGCGCAGCGCCGCGATTTCGCCGAAAACCACGAGTGCAACTTCGCCATCAGCGCGCGCGGCATCGGTCGT
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCCACCATCGA
TGACCTCAAGCTGCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGCTGTTCGTCGGTGCGACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAGTACATCCACCAGCACCAGAACTGCATCGTCACCCAGCGCGAGGTCGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGTCAGGCGCCGGACGTGATCCTCATCGGTGAGGTGCGCACCCGCGAGACCATGGACCACG
CCGTGGCATTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGCGGACCGGCAGAACCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACTCCGGATGGCAAGGGCCGCCGTGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCTGCCGACCTGA
TCCGCAAGGGTGAGGTGCACGAGCTCAAGGGCCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACACCCAGGGCGAGATCACCTACGAGGATGCCTTGCTCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAGACCGATGGCGACCACCTCACCAGCATGGCCAAGGGCCTGGAGCTGGAGGTCAGCGAGG
AAGATCCGGGGCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A5P9CD92

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	92.651	100	0.927
pilU	Acinetobacter baylyi ADP1	64.146	93.701	0.601
pilU	Vibrio cholerae strain A1552	58.571	91.864	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.75	88.189	0.386