Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   HPK14_RS25030 Genome accession   NZ_CP053662
Coordinates   5128295..5130124 (-) Length   609 a.a.
NCBI ID   WP_001377144.1    Uniprot ID   -
Organism   Escherichia coli strain eco-241     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5123295..5135124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPK14_RS25005 (HPK14_24735) bioP 5124013..5124912 (-) 900 WP_001196244.1 biotin transporter -
  HPK14_RS25010 (HPK14_24740) yigL 5124988..5125788 (-) 801 WP_000285353.1 sugar/pyridoxal phosphate phosphatase YigL -
  HPK14_RS25015 (HPK14_24745) pldB 5125796..5126818 (-) 1023 WP_000487648.1 lysophospholipase L2 -
  HPK14_RS25020 (HPK14_24750) rhtB 5126929..5127549 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  HPK14_RS25025 (HPK14_24755) rhtC 5127611..5128231 (-) 621 WP_000928828.1 threonine export protein RhtC -
  HPK14_RS25030 (HPK14_24760) recQ 5128295..5130124 (-) 1830 WP_001377144.1 ATP-dependent DNA helicase RecQ Machinery gene
  HPK14_RS25035 (HPK14_24765) pldA 5130257..5131126 (-) 870 WP_001259694.1 phospholipase A -
  HPK14_RS25040 (HPK14_24770) yigI 5131291..5131758 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  HPK14_RS25045 (HPK14_24775) rarD 5131810..5132700 (+) 891 WP_000339094.1 EamA family transporter RarD -
  HPK14_RS25050 (HPK14_24780) yigG 5132795..5133175 (+) 381 WP_001309870.1 protein YigG -
  HPK14_RS25055 (HPK14_24785) yigF 5133189..5133569 (+) 381 WP_000032585.1 DUF2628 domain-containing protein -
  HPK14_RS25060 (HPK14_24790) corA 5133612..5134562 (-) 951 WP_000947159.1 magnesium/cobalt transporter CorA -
  HPK14_RS25065 ysgD 5134714..5134770 (-) 57 WP_211180520.1 protein YsgD -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68393.53 Da        Isoelectric Point: 7.2544

>NTDB_id=393307 HPK14_RS25030 WP_001377144.1 5128295..5130124(-) (recQ) [Escherichia coli strain eco-241]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRSESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=393307 HPK14_RS25030 WP_001377144.1 5128295..5130124(-) (recQ) [Escherichia coli strain eco-241]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
TATGACAGGCTGTCGCACCGGGCAAATCCGCCTGCTTTATATCGCGCCGGAACGTCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCACACTGTATCTCCCAATGGGGTCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGTTTTCCGACGCTGCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGTTATGTACAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGTAATAGTCGTGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTTCGCGCTGATGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTGCATTTTGATATTCCGCGCAACATTGAATCCTAT
TATCAGGAAACCGGACGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCGGATATGGCGTG
GCTGCGTCGTTGTCTGGAAGAGAAACCGCAGGGGCAGTTACAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTGCTGCTGAACTACTTTGGCGAAGGGCGGCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAATACGATGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCAGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCCTTCGGCGGCAACTACGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCGACCTTGATTGAGATGGCTGAACAGATGCCGATCACTGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTCATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

RQC domain (RQC)

(406-514)

HRDC domain (HRDC)

(533-599)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.034

97.209

0.389

Multiple sequence alignment