Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   GCU50_RS18040 Genome accession   NZ_CP045300
Coordinates   3643836..3645269 (-) Length   477 a.a.
NCBI ID   WP_090121639.1    Uniprot ID   A0A1I7BK58
Organism   Kosakonia arachidis strain KACC 18508     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3638836..3650269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCU50_RS18010 elbB 3640404..3641057 (+) 654 WP_090121647.1 isoprenoid biosynthesis glyoxalase ElbB -
  GCU50_RS18015 mtgA 3641054..3641785 (+) 732 WP_090121645.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  GCU50_RS18020 npr 3641787..3642059 (-) 273 WP_090121644.1 PTS phosphocarrier protein NPr -
  GCU50_RS18025 rapZ 3642056..3642910 (-) 855 WP_090121642.1 RNase adapter RapZ -
  GCU50_RS18030 ptsN 3642953..3643444 (-) 492 WP_007369895.1 PTS IIA-like nitrogen regulatory protein PtsN -
  GCU50_RS18035 hpf 3643526..3643813 (-) 288 WP_090121641.1 ribosome hibernation promoting factor -
  GCU50_RS18040 rpoN/rpoN1 3643836..3645269 (-) 1434 WP_090121639.1 RNA polymerase factor sigma-54 Machinery gene
  GCU50_RS18045 lptB 3645318..3646043 (-) 726 WP_090121638.1 LPS export ABC transporter ATP-binding protein -
  GCU50_RS18050 lptA 3646050..3646604 (-) 555 WP_090121637.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  GCU50_RS18055 lptC 3646573..3647148 (-) 576 WP_090121635.1 LPS export ABC transporter periplasmic protein LptC -
  GCU50_RS18060 kdsC 3647145..3647711 (-) 567 WP_090121633.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  GCU50_RS18065 kdsD 3647726..3648712 (-) 987 WP_090121632.1 arabinose-5-phosphate isomerase KdsD -
  GCU50_RS18070 - 3648726..3649700 (-) 975 WP_090121631.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53932.78 Da        Isoelectric Point: 4.3913

>NTDB_id=393253 GCU50_RS18040 WP_090121639.1 3643836..3645269(-) (rpoN/rpoN1) [Kosakonia arachidis strain KACC 18508]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQADLHEEVDSQQTQENEGLDTVDALEQKEMPE
ELPLDTSWDEIYTAGTPSGSSHDYIDDELPIYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVTLED
VLESMGDEEVGLDEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFARDTPWCEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWMVELNSDSIPRLQINQHYASMGAGARNDADTQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=393253 GCU50_RS18040 WP_090121639.1 3643836..3645269(-) (rpoN/rpoN1) [Kosakonia arachidis strain KACC 18508]
ATGAAGCAAGGTTTGCAATTAAGGCTTAGCCAACAGCTAGCTATGACGCCGCAACTGCAACAGGCTATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTTCAGCAGGAACTTCAGCAAGCGCTGGAGAGTAATCCATTGCTGGAACAAGCCGATCTTCATG
AAGAAGTGGATAGCCAACAAACTCAGGAAAATGAAGGCCTGGATACTGTAGACGCGCTCGAACAGAAAGAGATGCCTGAA
GAGTTGCCGCTTGACACCAGTTGGGATGAGATTTATACCGCCGGAACACCTTCCGGTAGCAGCCATGATTATATCGATGA
TGAACTGCCGATTTATCAGGGTGAAACAACACAGTCCCTTCAGGATTACCTGATGTGGCAAGTGGAGTTAACGCCTTTTT
CCGATACCGATCGCGCCATCGCAACCTCTATTGTCGATGCTGTCGATGAGACCGGGTACCTTACCGTAACGCTTGAAGAT
GTACTGGAAAGCATGGGCGATGAAGAAGTCGGTCTTGATGAAGTTGAAGCGGTATTAAAGCGCGTTCAACGTTTCGATCC
GGTTGGCGTCGCTGCGCGCGATCTACGTGACTGCCTGTTGATCCAACTTTCCCAGTTCGCAAGAGATACGCCCTGGTGCG
AAGAGGCTCGCCTGATCATCAGCGATCACCTCGATCTGCTCGCCAACCACGACTTCCGTACGCTTATGCGCGTCACGCGT
CTTAAAGAAGATGTGCTGAAAGAAGCAGTCAATCTGATCCAGTCGCTGGATCCGCGCCCGGGTCAGTCGATCCAAACCGG
CGAACCAGAATATGTCATCCCGGACGTGCTAGTGCGTAAGCATAACGGGCGCTGGATGGTTGAACTGAACTCCGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCTTCAATGGGCGCTGGTGCTCGCAATGACGCCGACACCCAATTTATCCGC
AGTAATTTGCAGGAAGCGAAGTGGCTGATCAAAAGCCTTGAAAGTCGTAACGACACGCTATTGCGTGTCAGCCGCTGCAT
CGTCGAGCAGCAGCAGGCATTCTTTGAACAAGGTGAAGAGTTTATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAAATGCACGAATCCACGATTTCCCGTGTGACAACTCAGAAGTACCTGCACAGTCCTCGCGGCATTTTTGAACTGAAG
TATTTCTTCTCCAGCCATGTGAATACGGAAGGCGGCGGCGAAGCCTCGTCAACGGCCATTCGAGCGCTGGTTAAGAAATT
AATCGCCGCAGAAAACCCTGCAAAACCACTGAGCGACAGTAAGCTGACGACGCTACTTTCCGAGCAAGGAATTATGGTGG
CACGGCGTACCGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGTTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I7BK58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.331

100

0.488

Multiple sequence alignment