Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LV515_RS01500 Genome accession   NZ_CP045240
Coordinates   336322..337701 (+) Length   459 a.a.
NCBI ID   WP_003717514.1    Uniprot ID   C2ET76
Organism   Limosilactobacillus vaginalis strain LV515     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 331322..342701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LV515_RS01475 (LV515_01475) - 331776..333395 (-) 1620 WP_003717509.1 malolactic enzyme -
  LV515_RS01480 (LV515_01480) - 333527..334417 (+) 891 WP_003717510.1 LysR family transcriptional regulator -
  LV515_RS01485 (LV515_01485) rpiA 334511..335203 (-) 693 WP_003717511.1 ribose-5-phosphate isomerase RpiA -
  LV515_RS01490 (LV515_01490) - 335215..335508 (-) 294 WP_003717512.1 GNAT family N-acetyltransferase -
  LV515_RS01495 (LV515_01495) - 335613..336149 (+) 537 WP_003717513.1 hypothetical protein -
  LV515_RS01500 (LV515_01500) radA 336322..337701 (+) 1380 WP_003717514.1 DNA repair protein RadA Machinery gene
  LV515_RS01505 (LV515_01505) - 337723..338886 (+) 1164 WP_003717515.1 PIN/TRAM domain-containing protein -
  LV515_RS01510 (LV515_01510) - 338998..340656 (+) 1659 WP_003717516.1 formate--tetrahydrofolate ligase -
  LV515_RS01515 (LV515_01515) - 341313..341918 (+) 606 WP_240102037.1 Fic family protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50082.40 Da        Isoelectric Point: 8.9443

>NTDB_id=392867 LV515_RS01500 WP_003717514.1 336322..337701(+) (radA) [Limosilactobacillus vaginalis strain LV515]
MARVKTQYVCQNCGYNSPRYLGRCPNCGEWNTLVEEQIQSSSSSAATKRPTTTLTGLVAKPQKIGEINSHETPRVKTKLN
ELNRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSSEHHRVLYVSGEESATQIKMRAERLDVAADDFYIYPETNMDS
IKDTIRELKPEYVVIDSVQTMQATDVTSAIGSVSQIREVTAQLMQIAKSNNITIFVVGHVTKGGAIAGPKILEHMVDTVL
YFEGDLHHTYRILRSVKNRFGSTNELGIFEMHTNGLSEVKNPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALVTPT
VYGNAQRTATGLNRNRVSLIMAVLEKRANLMLQNQDAYLKAAGGVKLDEPAIDLAIAISIASSYRDKGTQATDAFVGEVG
LTGEIRRVNRIEQRVAEAQKLGFKRIFVPKNNLKGWQAPTGIEVIGVTTLRQALRLALG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=392867 LV515_RS01500 WP_003717514.1 336322..337701(+) (radA) [Limosilactobacillus vaginalis strain LV515]
ATGGCCAGAGTTAAAACACAATATGTTTGTCAGAATTGTGGATATAATTCACCACGATATTTAGGAAGATGTCCTAACTG
TGGTGAGTGGAATACGTTAGTTGAAGAACAAATCCAATCCTCAAGTTCCAGTGCAGCCACGAAACGACCAACCACGACGT
TAACTGGTTTGGTTGCTAAGCCACAGAAAATCGGTGAAATTAATAGTCACGAAACTCCCCGGGTAAAAACCAAACTGAAC
GAATTGAACCGGGTTCTTGGTGGCGGAATTGTTCCGGGGTCGTTAATCTTAATTGGTGGGGATCCTGGTATTGGAAAATC
AACGTTGCTCCTGCAAGTTTCTGGACAACTATCAAGTGAACATCACCGGGTTCTTTACGTTTCTGGGGAAGAAAGCGCCA
CTCAAATAAAAATGCGGGCAGAGCGGCTTGACGTGGCAGCAGATGACTTTTATATTTACCCAGAAACCAATATGGACAGT
ATTAAAGACACGATTCGTGAACTAAAACCAGAATACGTGGTGATTGATTCTGTTCAGACAATGCAGGCTACGGATGTCAC
TTCAGCGATCGGGAGTGTTTCGCAGATTCGTGAAGTAACCGCTCAGTTAATGCAAATTGCAAAGAGTAATAATATTACGA
TCTTTGTTGTTGGTCACGTTACTAAGGGCGGTGCAATCGCTGGACCAAAGATTTTGGAGCACATGGTTGATACTGTCTTG
TACTTTGAAGGAGATCTTCACCACACATATCGGATCCTCAGGTCAGTTAAAAACCGTTTTGGGTCAACTAATGAGCTGGG
AATTTTTGAAATGCATACGAATGGTTTATCAGAGGTGAAAAATCCTTCAGAAATTTTCCTCGAAGAACGATTAAAAGATG
CCACTGGCTCGGCTGTCGTTGTATCATTAGAAGGGACGCGGCCAATTCTTGTCGAAATTCAGGCTTTGGTGACACCGACC
GTCTATGGAAATGCTCAGCGAACTGCTACTGGTTTGAACCGAAATCGGGTATCATTGATAATGGCGGTTCTTGAAAAGCG
CGCCAATTTGATGCTCCAAAACCAGGACGCCTACCTTAAGGCTGCTGGTGGTGTAAAATTGGATGAGCCGGCAATCGACC
TGGCAATCGCAATTAGTATTGCATCTAGTTACCGGGATAAGGGGACACAAGCTACAGATGCATTTGTTGGTGAAGTTGGC
TTAACTGGTGAAATTCGGCGGGTTAACCGGATTGAACAGCGTGTTGCCGAGGCCCAGAAACTTGGCTTCAAACGGATCTT
TGTTCCGAAAAATAACTTGAAAGGGTGGCAAGCACCAACCGGGATCGAGGTTATCGGGGTAACCACCTTACGTCAGGCAT
TGCGTCTTGCGCTAGGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-432)

AAA domain (AAA_25)

(72-224)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C2ET76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

65.055

99.129

0.645
  radA Streptococcus mitis NCTC 12261

65.055

99.129

0.645
  radA Streptococcus pneumoniae Rx1

65.055

99.129

0.645
  radA Streptococcus pneumoniae D39

65.055

99.129

0.645
  radA Streptococcus pneumoniae R6

65.055

99.129

0.645
  radA Streptococcus pneumoniae TIGR4

65.055

99.129

0.645
  radA Bacillus subtilis subsp. subtilis str. 168

63.341

100

0.636

Multiple sequence alignment