Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GA693_RS00725 Genome accession   NZ_CP045198
Coordinates   57864..58664 (+) Length   266 a.a.
NCBI ID   WP_104489142.1    Uniprot ID   -
Organism   Acinetobacter indicus strain TQ23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 52864..63664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA693_RS00695 - 53195..53614 (+) 420 WP_005182377.1 hypothetical protein -
  GA693_RS00700 - 53782..55065 (+) 1284 WP_160241613.1 glycosyltransferase family 1 protein -
  GA693_RS00705 - 55098..55649 (+) 552 WP_104470590.1 phosphatase PAP2 family protein -
  GA693_RS00710 iscX 55744..55944 (+) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  GA693_RS00715 ndk 56054..56485 (+) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  GA693_RS00720 rlmN 56608..57843 (+) 1236 WP_005182389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  GA693_RS00725 pilF 57864..58664 (+) 801 WP_104489142.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  GA693_RS00730 - 58655..59443 (+) 789 WP_160241612.1 helix-turn-helix domain-containing protein -
  GA693_RS00735 ispG 59466..60587 (+) 1122 WP_005182396.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  GA693_RS00740 hisS 60591..61886 (+) 1296 WP_160241611.1 histidine--tRNA ligase -
  GA693_RS00745 - 61917..62621 (+) 705 WP_160241610.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30181.36 Da        Isoelectric Point: 9.2581

>NTDB_id=392595 GA693_RS00725 WP_104489142.1 57864..58664(+) (pilF) [Acinetobacter indicus strain TQ23]
MRKPSLKFICTSILGLTLVVSGGCQTTGAKGDPEKAVKVRTQLAAEYIKVNNFDAAKRALDQALEINSRDATANMMMGVL
LQQEGSKFNVERAERYFKQALSIEPKNAQARNNYGTYLYQVGRYNDAIEHLQIAGTTLGYDQRFRALENLGRAYVQLGKL
KEAEQAFIQALQINRDAYIAMLELAELKYFNQQQAMATELYEDFVRGVGQNNQDARALWIGIRIARANQDNLGTQVLVNQ
LRALYPESQEYQRYLQLKYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=392595 GA693_RS00725 WP_104489142.1 57864..58664(+) (pilF) [Acinetobacter indicus strain TQ23]
TTGAGAAAGCCAAGCTTGAAATTTATTTGCACTTCTATTTTGGGGCTGACCTTAGTTGTTTCTGGTGGATGTCAGACTAC
CGGTGCCAAAGGTGATCCAGAAAAAGCAGTAAAGGTAAGAACTCAACTGGCAGCTGAATACATTAAAGTCAATAACTTTG
ATGCGGCGAAACGTGCACTTGATCAGGCACTTGAGATTAATTCTCGCGATGCAACAGCCAATATGATGATGGGCGTGCTG
TTACAGCAAGAAGGAAGCAAGTTTAATGTAGAGCGAGCAGAGCGCTATTTTAAACAGGCACTTTCTATTGAGCCAAAAAA
TGCGCAGGCACGTAACAACTATGGTACGTATTTGTATCAGGTGGGGCGTTATAATGATGCGATTGAACATCTCCAAATAG
CAGGAACAACTTTGGGATATGATCAACGTTTTCGTGCATTGGAAAATTTAGGTCGAGCTTATGTGCAACTGGGTAAATTA
AAAGAGGCGGAACAAGCTTTTATTCAGGCACTACAGATTAATCGTGATGCCTATATTGCTATGCTTGAATTGGCTGAGTT
GAAGTATTTCAATCAGCAACAAGCAATGGCGACAGAACTTTATGAAGATTTTGTACGCGGTGTCGGGCAAAATAATCAAG
ATGCTCGTGCTTTATGGATCGGGATTCGTATTGCTCGAGCAAACCAAGACAATTTGGGGACACAGGTGTTGGTCAATCAA
CTGCGTGCCTTGTATCCAGAGAGTCAGGAATACCAACGTTATCTGCAATTGAAATACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(89-107)

Tetratricopeptide repeat (TPR_16)

(43-81)

Tetratricopeptide repeat (TPR_12)

(108-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.165

100

0.684
  pilF Acinetobacter baylyi ADP1

63.813

96.617

0.617

Multiple sequence alignment