Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   HO397_RS18735 Genome accession   NZ_CP053601
Coordinates   3869191..3871020 (+) Length   609 a.a.
NCBI ID   WP_012767774.1    Uniprot ID   -
Organism   Escherichia coli BL21     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3864191..3876020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HO397_RS18700 (HO397_18705) - 3864536..3865384 (-) 849 WP_001066390.1 YPO3983 family protein -
  HO397_RS18705 (HO397_18710) - 3865567..3865713 (+) 147 Protein_3690 CorA family divalent cation transporter -
  HO397_RS18710 (HO397_18715) yigF 3865754..3866134 (-) 381 WP_000032581.1 DUF2628 domain-containing protein -
  HO397_RS18715 (HO397_18720) yigG 3866148..3866528 (-) 381 Protein_3692 protein YigG -
  HO397_RS18720 (HO397_18725) rarD 3866623..3867513 (-) 891 WP_000339104.1 EamA family transporter RarD -
  HO397_RS18725 (HO397_18730) yigI 3867565..3868032 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  HO397_RS18730 (HO397_18735) pldA 3868197..3869066 (+) 870 WP_001259695.1 phospholipase A -
  HO397_RS18735 (HO397_18740) recQ 3869191..3871020 (+) 1830 WP_012767774.1 ATP-dependent DNA helicase RecQ Machinery gene
  HO397_RS18740 (HO397_18745) rhtC 3871084..3871704 (+) 621 WP_000928828.1 threonine export protein RhtC -
  HO397_RS18745 (HO397_18750) rhtB 3871766..3872386 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  HO397_RS18750 (HO397_18755) pldB 3872497..3873519 (+) 1023 WP_000487654.1 lysophospholipase L2 -
  HO397_RS18755 (HO397_18760) yigL 3873527..3874327 (+) 801 WP_000285349.1 sugar/pyridoxal phosphate phosphatase YigL -
  HO397_RS18760 (HO397_18765) bioP 3874403..3875302 (+) 900 WP_001196237.1 biotin transporter -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68405.59 Da        Isoelectric Point: 7.2544

>NTDB_id=392504 HO397_RS18735 WP_012767774.1 3869191..3871020(+) (recQ) [Escherichia coli BL21]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNIRVDVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=392504 HO397_RS18735 WP_012767774.1 3869191..3871020(+) (recQ) [Escherichia coli BL21]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGGGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCCTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
TATGACAGGCTGTCGCACCGGGCAAATCCGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGGTTCCCGACGCTCCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGGCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGATCGCCCGAACATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATATTCGCGTCGACGTGCAGGAAAAATTTCAGCGCGATGACCTGCAAATTGTGGTGGCGACCG
TGGCGTTTGGCATGGGCATCAACAAGCCTAACGTCCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCCGATATGGCGTG
GCTACGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAATTGCAGGATATCGAGCGCCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTGCTGCTGAACTACTTTGGCGAAGGGCGGCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAATACGATGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTATTTAACGAC
GCAACCTTGATTGAGATGGCGGAACAGATGCCGATCACTGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGCGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

DEAD/DEAH box helicase (DEAD)

(28-187)

HRDC domain (HRDC)

(533-599)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.034

97.209

0.389