Detailed information NTG0392473

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: HO397_RS13045
• Genome accession: NZ_CP053601
• Coordinates: 2676987..2677979 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli BL21
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2671987..2682979

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HO397_RS13025 (HO397_13030)	otnK	2672762..2674024 (+)	1263	WP_000590371.1	3-oxo-tetronate kinase	-
HO397_RS13030 (HO397_13035)	ygbL	2674021..2674659 (+)	639	WP_001278994.1	aldolase	-
HO397_RS13035 (HO397_13040)	ygbM	2674664..2675440 (+)	777	WP_001136925.1	HPr family phosphocarrier protein	-
HO397_RS13040 (HO397_13045)	ygbN	2675529..2676893 (+)	1365	WP_000104459.1	GntP family transporter	-
HO397_RS13045 (HO397_13050)	rpoS	2676987..2677979 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
HO397_RS13050 (HO397_13055)	nlpD	2678042..2679136 (-)	1095	WP_224692580.1	murein hydrolase activator NlpD	-
HO397_RS13055 (HO397_13060)	pcm	2679321..2679947 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
HO397_RS13060 (HO397_13065)	surE	2679941..2680702 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
HO397_RS13065 (HO397_13070)	truD	2680683..2681732 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
HO397_RS13070 (HO397_13075)	ispF	2681729..2682208 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
HO397_RS13075 (HO397_13080)	ispD	2682208..2682918 (-)	711	WP_000246144.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=392473 HO397_RS13045 WP_000081550.1 2676987..2677979(-) (rpoS) [Escherichia coli BL21]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=392473 HO397_RS13045 WP_000081550.1 2676987..2677979(-) (rpoS) [Escherichia coli BL21]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715