Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D1010_RS05550 Genome accession   NZ_CP045143
Coordinates   1127322..1128734 (+) Length   470 a.a.
NCBI ID   WP_063516467.1    Uniprot ID   A0A5P2TUH1
Organism   Schleiferilactobacillus harbinensis strain M1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1122322..1133734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1010_RS05520 (D1010_05520) - 1122494..1123213 (+) 720 WP_152260427.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  D1010_RS05525 (D1010_05525) - 1123182..1124525 (-) 1344 WP_152260428.1 D-serine ammonia-lyase -
  D1010_RS05530 (D1010_05530) - 1124510..1125154 (-) 645 WP_193312169.1 endonuclease III domain-containing protein -
  D1010_RS05535 (D1010_05535) - 1125285..1126019 (+) 735 WP_027827470.1 YebC/PmpR family DNA-binding transcriptional regulator -
  D1010_RS05540 (D1010_05540) - 1126096..1126374 (-) 279 WP_027827469.1 GNAT family N-acetyltransferase -
  D1010_RS05545 (D1010_05545) - 1126721..1127260 (+) 540 WP_027829450.1 dUTP diphosphatase -
  D1010_RS05550 (D1010_05550) radA 1127322..1128734 (+) 1413 WP_063516467.1 DNA repair protein RadA Machinery gene
  D1010_RS05555 (D1010_05555) - 1128731..1129870 (+) 1140 WP_027829449.1 PIN/TRAM domain-containing protein -
  D1010_RS05560 (D1010_05560) gltX 1129937..1131448 (+) 1512 WP_063516466.1 glutamate--tRNA ligase -
  D1010_RS05565 (D1010_05565) cysS 1131571..1132980 (+) 1410 WP_152260430.1 cysteine--tRNA ligase -
  D1010_RS05570 (D1010_05570) - 1132977..1133378 (+) 402 WP_063516465.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 470 a.a.        Molecular weight: 50704.12 Da        Isoelectric Point: 7.1739

>NTDB_id=392307 D1010_RS05550 WP_063516467.1 1127322..1128734(+) (radA) [Schleiferilactobacillus harbinensis strain M1]
MVKTKSRYVCQNCGYVSPQYFGRCPNCGAWNQMVEEVYTADANKKVGGSSNGVQSQPVALDAVNLDDNKRIPTHISELDR
VLGGGVVPGSLVLIGGDPGIGKSTLLMQVSGQLAKTSGEQVLYVSGEESASQIRLRAERLGVGGKHVVVYPETDMDLIRR
AIKEVKPGFLIIDSVQTMNEPALASAAGSVSQVREVTNELMRIAKNDGLTTFVVGHVTKDGAIAGPKMLEHMVDTVLYFE
GDMHHTYRILRAVKNRYGSTNEIGIFSMEEDGLHVVDNPSESFLEERLPHATGSAIVVSMEGTRPILVEIQALMSPTLFG
NAKRTTTGIDHNRAALIMAVLEKRANLLLQNQDAYLKATGGVKLDEPAIDLAIAMAVASSYQDAEVDPGDCFVGEIGLTG
EIRRVNRIESRIEEAAKLGFKRIFVPKNNLSRYKPTAKIQVVGVTTITQCLQQALKPSKAALAEKGGRGA

Nucleotide


Download         Length: 1413 bp        

>NTDB_id=392307 D1010_RS05550 WP_063516467.1 1127322..1128734(+) (radA) [Schleiferilactobacillus harbinensis strain M1]
ATGGTCAAAACAAAAAGCCGCTATGTCTGTCAAAACTGCGGTTATGTGTCACCGCAATATTTCGGCCGCTGCCCCAACTG
CGGCGCTTGGAACCAAATGGTGGAAGAGGTCTACACCGCGGATGCCAACAAAAAAGTGGGCGGCAGCAGCAATGGCGTGC
AAAGTCAGCCTGTTGCCTTGGATGCCGTTAATCTGGATGATAACAAGCGCATCCCAACGCACATTTCTGAACTCGACCGC
GTCTTGGGGGGCGGGGTCGTGCCGGGGTCGCTGGTCTTGATCGGCGGTGATCCGGGTATTGGCAAGTCTACTCTGCTGAT
GCAGGTTTCTGGCCAGTTGGCCAAGACCAGCGGCGAACAGGTGCTGTACGTCAGTGGGGAAGAAAGCGCGAGCCAGATTC
GCCTGCGGGCTGAACGGCTGGGGGTTGGCGGCAAGCACGTCGTCGTTTATCCGGAAACCGATATGGATTTGATTCGCCGG
GCAATCAAAGAGGTCAAGCCGGGTTTTCTAATCATTGATTCTGTTCAGACGATGAATGAACCGGCCCTAGCCTCAGCCGC
TGGCAGCGTCAGTCAGGTGCGGGAAGTGACCAATGAGTTGATGCGCATCGCGAAAAACGATGGGCTGACGACGTTTGTTG
TTGGGCACGTGACTAAAGATGGGGCGATCGCCGGGCCAAAAATGTTGGAGCACATGGTCGATACCGTTCTATACTTTGAG
GGGGACATGCACCATACGTATCGAATCTTGCGTGCCGTGAAGAACCGGTATGGGTCCACCAATGAGATCGGTATTTTCTC
CATGGAAGAAGATGGCCTGCACGTGGTGGACAACCCCTCCGAGTCGTTTTTGGAGGAACGCTTGCCCCATGCGACGGGAT
CGGCCATCGTGGTGTCCATGGAGGGCACACGGCCGATTTTGGTTGAGATTCAGGCTTTGATGTCCCCAACACTGTTCGGC
AATGCCAAGCGAACCACCACAGGCATCGACCACAACCGGGCAGCCTTGATCATGGCGGTACTGGAAAAGCGGGCGAATCT
GCTGTTGCAGAATCAGGATGCTTATCTGAAGGCCACTGGCGGGGTGAAGTTGGATGAACCGGCCATTGATTTGGCGATTG
CCATGGCAGTGGCCTCTAGTTACCAAGACGCCGAAGTCGATCCTGGGGATTGTTTCGTGGGCGAAATCGGTTTGACCGGT
GAAATCCGCCGGGTGAATCGGATCGAGAGCCGGATTGAAGAAGCGGCCAAGCTGGGCTTCAAACGGATCTTTGTTCCGAA
AAATAATTTATCTCGCTATAAGCCCACTGCGAAGATTCAGGTCGTGGGGGTCACGACGATCACTCAGTGCCTGCAACAGG
CGCTGAAACCAAGTAAAGCTGCATTAGCAGAGAAAGGGGGCCGTGGCGCATGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(64-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P2TUH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

64.912

97.021

0.63
  radA Streptococcus mitis SK321

64.912

97.021

0.63
  radA Streptococcus pneumoniae Rx1

64.693

97.021

0.628
  radA Streptococcus pneumoniae D39

64.693

97.021

0.628
  radA Streptococcus pneumoniae R6

64.693

97.021

0.628
  radA Streptococcus pneumoniae TIGR4

64.693

97.021

0.628
  radA Bacillus subtilis subsp. subtilis str. 168

60.699

97.447

0.591

Multiple sequence alignment