Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GB851_RS09555 Genome accession   NZ_CP045141
Coordinates   1832463..1833926 (-) Length   487 a.a.
NCBI ID   WP_164538333.1    Uniprot ID   -
Organism   Kingella kingae strain F41215CHC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1827463..1838926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GB851_RS09530 (GB851_09725) - 1828927..1829253 (-) 327 WP_223877612.1 hypothetical protein -
  GB851_RS09535 (GB851_09730) ubiM 1829309..1830493 (+) 1185 WP_174259096.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  GB851_RS09540 (GB851_09735) - 1830765..1831070 (+) 306 WP_003788039.1 LysE family transporter -
  GB851_RS09545 - 1831067..1831231 (-) 165 WP_155114354.1 hypothetical protein -
  GB851_RS09550 (GB851_09740) lpxB 1831252..1832403 (+) 1152 WP_003792117.1 lipid-A-disaccharide synthase -
  GB851_RS09555 (GB851_09745) pilA 1832463..1833926 (-) 1464 WP_164538333.1 signal recognition particle-docking protein FtsY Machinery gene
  GB851_RS09560 (GB851_09750) - 1834211..1834975 (+) 765 WP_206520650.1 DDE-type integrase/transposase/recombinase -
  GB851_RS09565 (GB851_09755) - 1834905..1835393 (-) 489 WP_003792114.1 toxin-activating lysine-acyltransferase -
  GB851_RS09570 (GB851_09760) - 1835683..1837110 (-) 1428 WP_003788049.1 HlyD family type I secretion periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 52504.00 Da        Isoelectric Point: 4.1946

>NTDB_id=392293 GB851_RS09555 WP_164538333.1 1832463..1833926(-) (pilA) [Kingella kingae strain F41215CHC]
MFNLFNKLFNRKAETPPNTEKEPEQVQAASAPETAPAVANENVQAALSAETEVAQENTADEVPQAEAESTETAEENVQAA
LNAETEVAQENTVEAEPMRHSWATPVVEEVKETVQSAEQWAEDMAHAVAEKADELEMRAEILAEETSESVQTLEHALEGE
NALPEPELLPEEPVAPEAEESLGWTARLARGLSKSRNQMAKSLAGVFGGGKIDEDLYEELETVLITSDMGIEATEQLMNE
VRNRVSLKGLKDGAELRSALKDAIYDLLKPLEQPLVLPDNGQPFVIMMAGINGAGKTTSIGKLAKYFQSQGKSVMLAAGD
TFRAAAREQLQEWGARNNVTVISQEKGDSAAVCFDAVEAAKARKIDVVLADTAGRLPTQLHLMEEIKKVKRVLQKSMPDA
PHEIMVVLDANIGQNAVNQVVAFDDALGVTGLIVTKLDGTAKGGVLAALVSSRAIPVRYIGVGESIDDLRPFNAKAFVDA
LLDEWSA

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=392293 GB851_RS09555 WP_164538333.1 1832463..1833926(-) (pilA) [Kingella kingae strain F41215CHC]
ATGTTTAATTTATTCAATAAACTGTTCAACCGGAAAGCCGAAACGCCGCCCAACACCGAAAAAGAACCAGAACAGGTGCA
GGCTGCTTCTGCGCCCGAAACTGCGCCTGCGGTTGCCAATGAAAATGTGCAGGCTGCTTTGAGTGCAGAAACCGAAGTCG
CGCAAGAAAACACGGCAGATGAAGTACCACAAGCTGAAGCCGAAAGCACGGAAACCGCAGAAGAAAATGTGCAGGCTGCT
TTGAACGCAGAAACCGAAGTCGCACAAGAAAATACGGTTGAAGCAGAACCCATGCGCCATAGCTGGGCAACGCCTGTGGT
GGAAGAAGTCAAAGAAACGGTGCAATCTGCCGAGCAATGGGCGGAAGACATGGCGCACGCGGTGGCAGAAAAAGCCGATG
AATTGGAAATGCGTGCCGAAATCTTGGCAGAAGAAACCAGCGAAAGCGTGCAGACGTTGGAACACGCGCTGGAAGGCGAA
AATGCGCTGCCCGAACCCGAATTGTTGCCCGAAGAGCCAGTCGCGCCTGAAGCGGAAGAATCCTTGGGCTGGACGGCACG
TTTGGCGCGTGGTTTGAGCAAATCGCGCAATCAAATGGCAAAGTCGTTGGCTGGCGTGTTTGGCGGCGGCAAAATTGACG
AAGATTTGTACGAAGAACTGGAAACGGTGCTGATTACCAGCGATATGGGCATTGAAGCGACCGAACAGTTGATGAACGAA
GTCCGCAATCGTGTGTCGCTCAAAGGCTTGAAAGACGGCGCGGAATTGCGTTCGGCACTCAAAGACGCGATTTATGATTT
GCTCAAACCATTGGAGCAGCCGCTGGTGTTGCCCGATAATGGTCAGCCGTTTGTGATTATGATGGCTGGCATCAATGGCG
CAGGCAAAACCACGTCTATTGGCAAATTGGCGAAGTATTTTCAATCGCAAGGCAAAAGTGTGATGTTGGCGGCGGGCGAT
ACCTTCCGTGCGGCGGCGCGTGAGCAGTTGCAAGAATGGGGTGCGCGAAACAATGTTACCGTGATTTCGCAAGAAAAAGG
CGACAGCGCGGCGGTGTGTTTTGATGCGGTGGAAGCGGCAAAAGCGCGTAAAATTGACGTGGTGCTGGCGGACACGGCTG
GACGCTTGCCAACGCAATTACACTTGATGGAAGAAATCAAAAAAGTGAAGCGCGTGTTGCAAAAATCCATGCCCGATGCG
CCACACGAAATCATGGTGGTGCTGGACGCGAACATCGGTCAAAACGCGGTCAATCAAGTGGTGGCGTTTGACGATGCGTT
GGGCGTAACAGGTTTGATTGTTACCAAGCTGGACGGCACGGCAAAAGGCGGCGTGTTGGCGGCGTTGGTAAGCAGTCGTG
CGATTCCTGTGCGCTATATTGGCGTGGGCGAGAGCATTGACGATTTGCGCCCGTTCAATGCCAAAGCGTTTGTGGACGCG
CTGTTGGACGAGTGGTCGGCATAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(199-263)

SRP54-type protein, GTPase domain (SRP54)

(283-482)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

72.679

77.413

0.563

Multiple sequence alignment