Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   HO399_RS02560 Genome accession   NZ_CP053599
Coordinates   557207..559036 (-) Length   609 a.a.
NCBI ID   WP_012767774.1    Uniprot ID   -
Organism   Escherichia coli strain NEBExpress     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 552207..564036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HO399_RS02535 (HO399_02535) bioP 552925..553824 (-) 900 WP_001196237.1 biotin transporter -
  HO399_RS02540 (HO399_02540) yigL 553900..554700 (-) 801 WP_000285349.1 sugar/pyridoxal phosphate phosphatase YigL -
  HO399_RS02545 (HO399_02545) pldB 554708..555730 (-) 1023 WP_000487654.1 lysophospholipase L2 -
  HO399_RS02550 (HO399_02550) rhtB 555841..556461 (+) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  HO399_RS02555 (HO399_02555) rhtC 556523..557143 (-) 621 WP_000928828.1 threonine export protein RhtC -
  HO399_RS02560 (HO399_02560) recQ 557207..559036 (-) 1830 WP_012767774.1 ATP-dependent DNA helicase RecQ Machinery gene
  HO399_RS02565 (HO399_02565) pldA 559161..560030 (-) 870 WP_001259695.1 phospholipase A -
  HO399_RS02570 (HO399_02570) yigI 560195..560662 (+) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  HO399_RS02575 (HO399_02575) rarD 560714..561604 (+) 891 WP_000339104.1 EamA family transporter RarD -
  HO399_RS02580 (HO399_02580) yigG 561699..562079 (+) 381 Protein_501 protein YigG -
  HO399_RS02585 (HO399_02585) yigF 562093..562473 (+) 381 WP_000032581.1 DUF2628 domain-containing protein -
  HO399_RS02590 (HO399_02590) - 562514..562660 (-) 147 Protein_503 CorA family divalent cation transporter -
  HO399_RS02595 (HO399_02595) - 562843..563691 (+) 849 WP_001066390.1 YPO3983 family protein -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68405.59 Da        Isoelectric Point: 7.2544

>NTDB_id=392286 HO399_RS02560 WP_012767774.1 557207..559036(-) (recQ) [Escherichia coli strain NEBExpress]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNIRVDVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=392286 HO399_RS02560 WP_012767774.1 557207..559036(-) (recQ) [Escherichia coli strain NEBExpress]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGGGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGCCGCGATTGCCTCGTCGTCATGCCCACTGGTGGCGGAAAAT
CCCTTTGCTATCAAATCCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCCTTGATGAAAGAT
CAGGTGGATCAACTGCAAGCCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAGCAGCAACTTGAAGT
TATGACAGGCTGTCGCACCGGGCAAATCCGCCTGCTTTATATCGCGCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATACGCTGCCCTCGGTCAGCTGCGTCAGCGGTTCCCGACGCTCCCGTTTATGGCGCTGACTGCCACAGCCGATGACAC
CACGCGGCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGATCGCCCGAACATTC
GCTACATGCTGATGGAGAAATTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAACGCGGTAAGTCGGGGATT
ATTTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGAATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATATTCGCGTCGACGTGCAGGAAAAATTTCAGCGCGATGACCTGCAAATTGTGGTGGCGACCG
TGGCGTTTGGCATGGGCATCAACAAGCCTAACGTCCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCCGATATGGCGTG
GCTACGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAATTGCAGGATATCGAGCGCCATAAACTCAATGCGATGGGCGCGT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTGCTGCTGAACTACTTTGGCGAAGGGCGGCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAATACGATGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGC
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATTGCCGATGAAAGCAATGTCCCGCCGTACGTGGTATTTAACGAC
GCAACCTTGATTGAGATGGCGGAACAGATGCCGATCACTGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTCGATGGCGACGACGAAGAGTAG

Domains

Predicted by InterProScan.

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

DEAD/DEAH box helicase (DEAD)

(28-187)

HRDC domain (HRDC)

(533-599)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.034

97.209

0.389

Multiple sequence alignment