Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GB851_RS06145 Genome accession   NZ_CP045141
Coordinates   1190874..1192250 (-) Length   458 a.a.
NCBI ID   WP_003789855.1    Uniprot ID   -
Organism   Kingella kingae strain F41215CHC     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1185874..1197250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GB851_RS06135 (GB851_06295) - 1187199..1189970 (+) 2772 WP_164538199.1 DNA methyltransferase -
  GB851_RS06140 (GB851_06300) - 1190272..1190610 (-) 339 WP_003789856.1 hypothetical protein -
  GB851_RS06145 (GB851_06305) radA 1190874..1192250 (-) 1377 WP_003789855.1 DNA repair protein RadA Machinery gene
  GB851_RS06150 - 1192333..1192476 (-) 144 WP_019389203.1 hypothetical protein -
  GB851_RS06155 (GB851_06310) - 1192553..1192801 (-) 249 WP_003789854.1 hypothetical protein -
  GB851_RS06160 (GB851_06315) proC 1193262..1194056 (+) 795 WP_038311003.1 pyrroline-5-carboxylate reductase -
  GB851_RS06165 (GB851_06320) - 1194092..1194850 (-) 759 WP_038311005.1 ATP-binding cassette domain-containing protein -
  GB851_RS06170 (GB851_06325) glnA 1194967..1196385 (-) 1419 WP_038311007.1 type I glutamate--ammonia ligase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49421.12 Da        Isoelectric Point: 7.6114

>NTDB_id=392283 GB851_RS06145 WP_003789855.1 1190874..1192250(-) (radA) [Kingella kingae strain F41215CHC]
MAKAPKIQFQCTECGGTTPKWVGKCPHCGEWNTLSEQLTAPEPKNARFQSWAAETTQVQELSKVTAMEVPREATGMGELD
RVLGGGLVDGAVILLGGDPGIGKSTLLLQTIALMAKKRKVLYVSGEESAQQVALRSQRLGLNADGVNLLAEIRLEAIQAA
LKQHEPSVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRIAKQMGIAMIFVGHVTKDGAIAGPRVLEHMVDTVLYFEG
DQHSNYRMIRAIKNRFGAANELGVFAMTETGLKGVSNPSAIFLASYRDDVAGSCVLVTQEGSRPLLVEIQALVDDAHGFT
PKRLTVGLEQNRLAMLLAVLNRHAGVACFDQDVFLNAVGGVKISEPAADLAVILAMLSSYRNKPLPEKMVAFGEIGLSGE
IRPVPRGQERLKEAEKLGFKRAIVPKANLPKNLKEFPSLKIQGVSSLQEAVNACRDWE

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=392283 GB851_RS06145 WP_003789855.1 1190874..1192250(-) (radA) [Kingella kingae strain F41215CHC]
ATGGCAAAAGCCCCAAAAATCCAATTCCAATGCACCGAATGCGGTGGCACAACACCCAAATGGGTTGGCAAATGCCCGCA
TTGTGGCGAATGGAACACGCTTTCTGAACAGCTAACCGCACCCGAACCCAAAAACGCGCGTTTCCAATCGTGGGCGGCGG
AAACCACGCAAGTGCAAGAATTATCCAAAGTTACCGCCATGGAAGTCCCACGCGAAGCCACTGGCATGGGCGAACTTGAC
CGCGTGTTGGGCGGCGGTTTGGTGGACGGTGCGGTCATTTTGCTTGGTGGCGACCCAGGGATTGGCAAATCCACATTGTT
GTTGCAAACCATTGCTTTAATGGCGAAAAAACGCAAGGTGTTGTATGTGTCGGGCGAGGAATCGGCGCAACAAGTGGCGT
TGCGTTCGCAGCGTTTGGGGCTGAATGCGGATGGCGTGAATTTGTTGGCGGAAATCCGTTTGGAGGCGATTCAGGCTGCC
TTAAAACAGCACGAGCCGTCTGTGGTGGTGATTGACTCGATTCAAACGATGTATTCCGACCAAATCACGTCCGCGCCTGG
CTCGGTGTCGCAGGTGCGCGAATGTGCGGCGCAACTGACACGAATTGCGAAACAAATGGGCATTGCGATGATTTTTGTTG
GACACGTTACCAAAGACGGCGCGATTGCAGGTCCGCGCGTGTTGGAGCATATGGTGGACACGGTGCTGTATTTTGAGGGC
GACCAGCATTCCAATTACCGCATGATTCGGGCGATTAAAAACCGTTTTGGAGCGGCTAATGAATTGGGCGTGTTTGCGAT
GACGGAAACGGGCTTGAAAGGCGTTTCTAATCCGTCTGCGATTTTTTTGGCGAGCTATCGCGATGATGTGGCGGGTTCGT
GCGTGTTGGTTACGCAAGAGGGCAGTCGTCCGCTTTTGGTGGAAATTCAGGCGCTTGTTGATGATGCGCACGGTTTCACG
CCGAAGCGTTTAACGGTGGGTTTGGAGCAAAACCGTCTTGCCATGTTGCTGGCGGTGCTGAATCGCCACGCTGGCGTTGC
GTGTTTTGACCAAGATGTGTTTTTGAATGCGGTCGGCGGCGTGAAAATCAGCGAACCTGCGGCGGATTTGGCGGTAATTT
TGGCGATGTTGTCGAGCTATCGCAATAAGCCGCTGCCTGAAAAAATGGTGGCGTTTGGGGAAATTGGTTTGAGTGGCGAA
ATTCGTCCTGTGCCACGTGGGCAGGAGCGTTTGAAAGAAGCGGAGAAATTGGGCTTTAAACGGGCGATTGTGCCGAAAGC
GAATTTGCCGAAGAATTTAAAAGAGTTCCCAAGCCTGAAAATTCAAGGCGTAAGCAGCCTGCAAGAAGCGGTGAATGCTT
GTCGGGATTGGGAATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(361-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(79-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.33

99.345

0.5
  radA Streptococcus mitis SK321

48.786

98.908

0.483
  radA Streptococcus pneumoniae Rx1

48.246

99.563

0.48
  radA Streptococcus pneumoniae D39

48.246

99.563

0.48
  radA Streptococcus pneumoniae R6

48.246

99.563

0.48
  radA Streptococcus pneumoniae TIGR4

48.246

99.563

0.48
  radA Streptococcus mitis NCTC 12261

48.115

98.472

0.474

Multiple sequence alignment