Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GA597_RS12545 Genome accession   NZ_CP045137
Coordinates   2492502..2493866 (-) Length   454 a.a.
NCBI ID   WP_011276735.1    Uniprot ID   A0A2A1KAD2
Organism   Staphylococcus haemolyticus strain VB5326     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2487502..2498866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GA597_RS12530 (GA597_04345) cysE 2488426..2489055 (-) 630 WP_172458793.1 serine O-acetyltransferase -
  GA597_RS12535 (GA597_04340) gltX 2489510..2490964 (-) 1455 WP_119890406.1 glutamate--tRNA ligase -
  GA597_RS12540 (GA597_04335) - 2491409..2492476 (-) 1068 WP_011276734.1 PIN/TRAM domain-containing protein -
  GA597_RS12545 (GA597_04330) radA 2492502..2493866 (-) 1365 WP_011276735.1 DNA repair protein RadA Machinery gene
  GA597_RS12550 (GA597_04325) - 2494090..2496564 (-) 2475 WP_016931338.1 ATP-dependent Clp protease ATP-binding subunit -
  GA597_RS12555 (GA597_04320) - 2496580..2497587 (-) 1008 WP_011276737.1 protein arginine kinase -
  GA597_RS12560 (GA597_04315) - 2497580..2498143 (-) 564 WP_011276738.1 UvrB/UvrC motif-containing protein -
  GA597_RS12565 (GA597_04310) - 2498166..2498627 (-) 462 WP_011276739.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49851.15 Da        Isoelectric Point: 7.0044

>NTDB_id=392266 GA597_RS12545 WP_011276735.1 2492502..2493866(-) (radA) [Staphylococcus haemolyticus strain VB5326]
MAKKKVIFECMACGYQSPKWMGKCPNCGSWNQMEEIVEKASNPKHGVKTSTSSAKVQKLNDIKNEVTPRVQTESKEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKLNVLYITGEESLNQTKLRADRLDEDSSNLNVLAETDLEVIHQTVQ
QAQPDILVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVHNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFERVIIPQTNIGGWQFPEGIKVVGVSTVHDALKYAFKS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=392266 GA597_RS12545 WP_011276735.1 2492502..2493866(-) (radA) [Staphylococcus haemolyticus strain VB5326]
TTGGCCAAAAAGAAAGTGATTTTTGAGTGTATGGCTTGCGGTTATCAATCTCCAAAATGGATGGGAAAGTGTCCTAATTG
TGGGTCATGGAATCAAATGGAGGAAATTGTTGAAAAAGCTTCAAATCCTAAGCACGGCGTAAAGACATCAACGTCTAGCG
CGAAAGTCCAGAAATTAAATGATATCAAGAATGAAGTGACACCGAGAGTTCAGACAGAATCAAAAGAGTTCAACCGCGTT
TTAGGTGGTGGCATTGTAAGTGGTTCTTTAGTGTTAATAGGTGGAGATCCTGGTATCGGTAAATCAACGCTATTACTGCA
AATTTGTGCATCATTGTCACAAAAATTAAATGTACTGTATATTACAGGTGAAGAATCATTAAATCAAACTAAACTACGTG
CCGATCGACTAGATGAGGATTCGAGTAATTTAAATGTGCTAGCAGAAACGGACTTAGAAGTAATACATCAAACAGTTCAA
CAAGCGCAACCTGATATTTTAGTTGTGGATTCCATTCAAACAATATATCATCCAGAAATTAGTTCAGCACCTGGTTCTGT
GTCTCAAGTAAGAGAAAGTACACAGAGTTTAATGAATATTGCTAAACAAATGAATATTGCAACATTTATCGTCGGACATG
TTACGAAAGAAGGTCAAATTGCAGGACCACGTCTATTAGAGCATATGGTTGATACAGTATTGTACTTTGAGGGAGATGAG
CATCATGCTTATCGTATCTTGAGAGCGGTTAAGAACCGTTTTGGTTCTACTAATGAAATGGGCATTTTTGAGATGAAACA
AAGCGGATTAAAAGGCGTTCATAATCCTTCAGAAATGTTCTTAGAGGAACGTTCTACCAATGTGCCGGGATCAACGATTG
TAGCAACTATGGAAGGTACACGACCTCTATTAATAGAAGTTCAGGCCTTAGTGACGCCTACAACATTTAATAACCCTAGA
CGTATGGCAACAGGTATAGATCATAATCGTTTGAGTTTATTAATGGCTGTATTAGAGAAAAAAGAAAACTATTTATTACA
ACAACAAGATGCATACATAAAAGTAGCTGGTGGCGTACGATTGACAGAGCCGGCAGTTGATTTAAGTGTTATAGTAGCAA
CAGCTTCAAGTTTTAAAGATAAAGCAGTAGATGGGCTTGATTGTTATATTGGGGAAGTAGGTTTGACCGGTGAAGTGAGA
CGTGTTTCTCGAATTGAACAACGTGTTCAAGAAGCAGCTAAATTAGGCTTTGAACGTGTTATTATTCCACAGACAAATAT
CGGTGGATGGCAATTTCCTGAAGGCATTAAAGTAGTTGGAGTGTCTACAGTACATGACGCTTTAAAATATGCATTCAAAT
CGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-218)

Subunit ChlI of Mg-chelatase (ChlI)

(359-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A1KAD2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.427

100

0.659
  radA Streptococcus pneumoniae Rx1

60.177

99.559

0.599
  radA Streptococcus pneumoniae D39

60.177

99.559

0.599
  radA Streptococcus pneumoniae R6

60.177

99.559

0.599
  radA Streptococcus pneumoniae TIGR4

60.177

99.559

0.599
  radA Streptococcus mitis NCTC 12261

60.177

99.559

0.599
  radA Streptococcus mitis SK321

60.177

99.559

0.599

Multiple sequence alignment