Detailed information NTG0392236

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: HO400_RS08130
• Genome accession: NZ_CP053597
• Coordinates: 1723543..1724535 (+)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain T7Express_LysY
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1718543..1729535

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HO400_RS08100 (HO400_08100)	ispD	1718604..1719314 (+)	711	WP_000246144.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
HO400_RS08105 (HO400_08105)	ispF	1719314..1719793 (+)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
HO400_RS08110 (HO400_08110)	truD	1719790..1720839 (+)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
HO400_RS08115 (HO400_08115)	surE	1720820..1721581 (+)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
HO400_RS08120 (HO400_08120)	pcm	1721575..1722201 (+)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
HO400_RS08125 (HO400_08125)	nlpD	1722386..1723480 (+)	1095	WP_224692580.1	murein hydrolase activator NlpD	-
HO400_RS08130 (HO400_08130)	rpoS	1723543..1724535 (+)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
HO400_RS08135 (HO400_08135)	ygbN	1724629..1725993 (-)	1365	WP_000104459.1	GntP family transporter	-
HO400_RS08140 (HO400_08140)	ygbM	1726082..1726858 (-)	777	WP_001136925.1	HPr family phosphocarrier protein	-
HO400_RS08145 (HO400_08145)	ygbL	1726863..1727501 (-)	639	WP_001278994.1	aldolase	-
HO400_RS08150 (HO400_08150)	otnK	1727498..1728760 (-)	1263	WP_000590371.1	3-oxo-tetronate kinase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=392236 HO400_RS08130 WP_000081550.1 1723543..1724535(+) (rpoS) [Escherichia coli strain T7Express_LysY]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=392236 HO400_RS08130 WP_000081550.1 1723543..1724535(+) (rpoS) [Escherichia coli strain T7Express_LysY]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715