Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HO400_RS05215 Genome accession   NZ_CP053597
Coordinates   1131313..1131990 (-) Length   225 a.a.
NCBI ID   WP_000178163.1    Uniprot ID   -
Organism   Escherichia coli strain T7Express_LysY     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1126313..1136990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HO400_RS05195 (HO400_05195) tuf 1126837..1128021 (+) 1185 WP_000031783.1 elongation factor Tu -
  HO400_RS05200 (HO400_05200) - 1128313..1130373 (+) 2061 WP_000773160.1 carbohydrate-binding protein -
  HO400_RS05205 (HO400_05205) bfd 1130542..1130736 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  HO400_RS05210 (HO400_05210) bfr 1130808..1131284 (+) 477 WP_000675504.1 bacterioferritin -
  HO400_RS05215 (HO400_05215) pilD 1131313..1131990 (-) 678 WP_000178163.1 prepilin peptidase GspO Machinery gene
  HO400_RS05220 (HO400_05220) gspM 1131990..1132451 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  HO400_RS05225 (HO400_05225) gspL 1132448..1133611 (-) 1164 WP_001115228.1 type II secretion system protein GspL -
  HO400_RS05230 (HO400_05230) gspK 1133626..1134609 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  HO400_RS05235 (HO400_05235) gspJ 1134602..1135189 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  HO400_RS05240 (HO400_05240) gspI 1135182..1135559 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  HO400_RS05245 (HO400_05245) gspH 1135556..1136065 (-) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  HO400_RS05250 (HO400_05250) gspG 1136073..1136510 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24928.81 Da        Isoelectric Point: 7.9563

>NTDB_id=392225 HO400_RS05215 WP_000178163.1 1131313..1131990(-) (pilD) [Escherichia coli strain T7Express_LysY]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTSAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=392225 HO400_RS05215 WP_000178163.1 1131313..1131990(-) (pilD) [Escherichia coli strain T7Express_LysY]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTAGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment